Sort   by:  
 Page size 
ProteomicsInterdomain-linker control conformational transitions in the SLC23 elevator transporter UraA
Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate via the elevator alternating access mechanism and are composed of two rigid domains whose relative motion drives transport. The lack of large...
ORGANISM(S): Escherichia coli 
2024-10-17 | PXD054394 | Pride
Hdx-ms E. coli Uraa 
ProteomicsNeuronal proteome dynamics during homeostatic scaling.
Homeostatic scaling adjusts synaptic strength in response to persistent changes in neuronal network activity. This compensatory mechanism requires proteome remodeling accomplished via regulation of protein synthesis as well as degradation, but the global patterns of proteome remodeling and the under...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-03-31 | PXD016004 | Pride
Synaptic plasticity Proteome turnover Dynamic silac Proteome remodelling Homeostatic scaling 
ProteomicsNeuronal ribosomes dynamically exchange ribosomal proteins in a context-dependent manner.
Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-09-20 | PXD026973 | Pride
Neuronal ribosomes Prm Rrna Ribosome assembly Protein complex Stress H2o2 Ribosomal proteins Protein synthesis Translation Local translation Psilac Neurons Neuronal processes Silac Ribosome Ribosome biogenesis Oxidative stress Hydrogen peroxide 
ProteomicsAn Abundance of Free Proteasomal Regulatory (19S) Particles Regulate Neuronal Synapses Independent of the Proteasome
The major protein-degradation machine, the proteasome, functions at brain synapses and regulates long-term information storage. Here we found that the two essential subcomplexes of the proteasome, the regulatory (19S) and catalytic (20S) particles that assemble to recognize and degrade substrates, w...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-11-26 | PXD041161 | Pride
K48 In-gel digest Synapse Neurons Neuronal proteasome Native page 19s K63 Proteasome Ubiquitin 
ProteomicsMEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1
Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal different...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-07-21 | PXD007078 | Pride
Parp1 Meis Histone h1 
ProteomicsEssential protein P116 scavenges cholesterol and other indispensable lipids for Mycoplasmas
Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213...
ORGANISM(S): Bos taurus (Bovine) Escherichia coli 
2023-04-19 | PXD037758 | Pride
Mycoplasma P116 Timstof 
ProteomicsLocal and global influences on protein turnover in neurons and glia.
Proteins exhibit dynamics in their expression level in response to intracellular and extracellular signals. Regulation of protein turnover allows cells to rapidly and efficiently remodel their proteomes. It is known that proteins can show very different turnover rates in different tissue of the same...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-06-05 | PXD008596 | Pride
Proteome turnover Neurons Silac Glia 
ProteomicsPhosphorylation in synaptic plasticity
Homeostatic synaptic scaling is a compensatory mechanism that allows adjustment of the strength of synaptic connections up or down in response to changes in input to maintain global network stability. To characterize the molecular changes on the level of protein modification that contribute to synap...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-08-12 | PXD021834 | Pride
Phosphoproteomics Synaptic plasticity Bicuculline Neurons Tetrodotoxin Homeostatic scaling 
ProteomicsLigand binding and conformational dynamics of the E. coli nicotinamide nucleotide transhydrogenase revealed by hydrogen/deuterium exchange mass spectrometry
Nicotinamide nucleotide transhydrogenases are integral membrane proteins that utilizes the proton motive force to reduce NADP+ to NADPH while converting NADH to NAD+. Atomic structures of various transhydrogenases in different ligand-bound states have become available, and it is clear that the molec...
ORGANISM(S): Escherichia coli 
2022-10-26 | PXD037413 | Pride
Channel opening Membrane protein Small ligand binding Hdx-ms Cyclic ims‑ms 
ProteomicsTime- and Polarity-dependent Proteomic Changes associated with Homeostatic Scaling at Central Synapses
In homeostatic scaling, the cellular mechanisms that detect the offset from the set-point, the duration of the offset and implement a cellular response are not well-understood. To understand the time-dependent dynamics,we manipulated activity for 2 hrs to induce the process of up or down-scaling and...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-02-16 | PXD008271 | Pride
Boncat Proteomics Homeostatic scaling 
Sort   by:  
 Page size