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ProteomicsProteomics of Intracellular Freezing Survival
Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P....
ORGANISM(S): Panagrolaimus 
2020-05-01 | PXD018121 | Pride
Intracellular freezing Nematode Panagrolaimus Cryoprotection 
ProteomicsExtrarenal ?-klotho expression in human tissues
?-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of ?-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080719 | MassIVE
ProteomicsLOPIT-DC subcellular map of U-2 OS cells
Generation of a high-resolution subcellular localisation map for the proteome of the human cancer cell line U-2 OS, using the newly developed LOPIT-DC method. Comparison of the results generated using LOPIT-DC with data generated using the well-established hyperLOPIT method and data mining regarding...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-11-23 | MSV000088434 | MassIVE
TranscriptomicsDsbA plays a critical and multi-faceted role in the production of secreted virulence factors by Erwinia carotovora
Erwinia carotovora subsp. atroseptica (Eca) is an enterobacterial phytopathogen causing economically-significant soft rot disease. Pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the Type II (Out) secretion system. DsbA catalyses the introduction of dis...
ORGANISM(S): Pectobacterium carotovorum 
2010-06-20 | E-GEOD-10647 | biostudies-arrayexpress
ProteomicsOperation of a TCA cycle subnetwork in the mammalian nucleus
Nucleic acid and histone modifications critically depend on central metabolism for substrates and co-factors. Although a few enzymes related to the formation of these required metabolites have been reported in the nucleus, the corresponding metabolic pathways are considered to function elsewhere in ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD032918 | Pride
Succinylation Subcellular localisation Lopit Spatial proteomics 
ProteomicsAdditional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).
We investigated if additional peptide purification by traveling wave ion mobility separation (TWIMS) can reduce precursor contamination using a mixture of S. cerevisiae and HeLa proteomes. In accordance with previous reports on FAIMS-Orbitrap instruments, we find that TWIMS provides a remarkable imp...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2014-06-10 | PXD001047 | Pride
Ims Tmt Ion mobility separation Isobaric mass tag Tandem mass tag Travelling wave ion mobility separation Twims 
ProteomicsLocalisation of Organelle Proteins using Data-Independent Acquisition (DIA-LOP)
Subcellular localisation within the proteome fundamentally influences cellular processes, however the development of high-throughput techniques to allow proteome-wide mapping of the cell has proven difficult. Here we present DIA-LOP, an approach capable of high-throughput spatial proteome mapping wi...
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD063082 | Pride
Human Lc-msms Dia Diapasef Dda 
ProteomicsDynamic proteomic profiling of extra-embryonic endoderm differentiation in mouse embryonic stem cells
During mammalian pre-implantation development, the cells of the blastocyst’s inner cell mass differentiate into the epiblast and primitive endoderm lineages, which give rise to the fetus and extra-embryonic tissues, respectively. Extra-embryonic endoderm differentiation can be modeled in vitro by in...
ORGANISM(S): Mus musculus (Mouse) 
2015-06-13 | PXD001901 | Pride
Extra-embryonic endoderm (xen) stem cells Differentiation Tandem mass tags (tmt) Quantitative proteomics Pluripotency 
ProteomicsExtrarenal α-klotho expression in human tissues
α-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of...
ORGANISM(S): Homo sapiens (Human) 
2015-10-13 | PXD002775 | Pride
Human Prm Klotho Ontogeny 
ProteomicsHyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line
The hyperLOPIT proteomics platform combines mass-spectrometry with state-of-the-art machine learning for simultaneous “mapping” of the steady-state subcellular location of thousands of proteins. Here, we use a synergistic approach and combine global proteome analysis with hyperLOPIT in a fully Bayes...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD023509 | Pride
Hyperlopit Thp-1 cells Immune response Lc-ms/ms 
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