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ProteomicsSimple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples
In-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological...
ORGANISM(S): Homo sapiens (Human) 
2021-02-23 | PXD022313 | Pride
Micro-spe tip Omset Limited sample On-micro solid-phase extraction tip Sample preparation Stagetip 
ProteomicsReverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis
Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...
ORGANISM(S): Homo sapiens (Human) 
2022-06-16 | PXD023817 | Pride
Scx-tip Mass spectrometry Fractionation Chemical cross-linking 
UnknownSimple and Efficient Microsolid-Phase Extraction Tip-Based Sample Preparation Workflow to Enable Sensitive Proteomic Profiling of Limited Samples (200 to 10,000 Cells).
Not available
S-EPMC7954648 | biostudies-literature
MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsHigh throughput microfluidic 3D biomimetic model enabling quantitative description of human breast tumor microenvironment
Cancer is driven by genetic aberrations in the tumor cells and fundamental changes in the tumor microenvironment (TME). These changes offer potential targets for novel therapeutics, yet lack of in vitro 3D models recapitulating this complex microenvironment impedes such progress. Here, we generated ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD021509 | Pride
Breast cancer Microfluidics Tumor microenvironment Proteomic profiling Alginate 
ProteomicsDeconstructing the regulation of protein, cellular origin and tumour grade-specific N and O-glycosylation associated with prostate cancer progression
Glycobiology plays a central role in prostate cancer (PCa), as evidenced by the aberrant glycosylation in PCa-derived glycoproteins. Understanding the protein glycosylation changes underling PCa onset and progression may open opportunities for the discovery of novel biomarkers for PCa detection and ...
ORGANISM(S): Homo sapiens (Human) 
2021-03-07 | PXD019443 | Pride
Glycosylation Glycoproteome Mass spectrometry Prostate cancer 
ProteomicsP. falciparum merozoites phoshoproteome
Pathology of the most lethal form of malaria is caused by Plasmodium falciparum asexual blood stages and initiated by merozoite invasion of erythrocytes. We present a phosphoproteome analysis of extracellular merozoites revealing 1765 unique phosphorylation sites including 785 sites not previously d...
ORGANISM(S): Bos taurus (Bovine) Plasmodium falciparum (isolate 3D7) 
2015-04-23 | PXD001684 | Pride
Merozoites  plasmodium falciparum  malaria  phosphorylation 
ProteomicsPersistent mirusvirus infection in the marine protist Aurantiochytrium
The protist Aurantiochytrium limacinum possesses two mirusvirus-like genomic elements, one a circular episome (AurliV-1) and the other (AurliV-2) a chromosomal integrant. Here we show that genes in both genomes are expressed and virions containing mainly AurliV-1 DNA are produced under starvation co...
ORGANISM(S): Aurantiochytrium limacinum ATCC MYA-1381 
2025-09-23 | PXD060676 | Pride
Mirusvirus Aurantiochytrium 
ProteomicsAntiviral HCV Treatment Induces Rapid Proteomic and Phospho-proteomic Changes in Hepatic Interferon and ERK1/2 Signaling Pathways
Although treatment of chronic hepatitis C virus (HCV) infection with direct acting antivirals (DAAs) results in high rates of cure, liver fibrosis does not resolve immediately after HCV eradication. Resolution of fibrosis occurs in some, but not all patients, after HCV cure, and hepatic decompensati...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD024846 | Pride
Phosphoproteomics Hcv Liver biopsy Proteomics Daa 
ProteomicsFirst dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure
Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Ent...
ORGANISM(S): Escherichia coli 
2018-07-11 | PXD008019 | Pride
Carbapenemases Kpc Proteomics Swath analysis Imp Antibiotic resistance Ndm 
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