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ProteomicsCharacterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling
Supplemental data for the article: Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling Eunkyung An, Manikandan Narayanan, and Aleksandra Nita-Lazar* *corresponding author: Cellular Networks Proteomics Unit Laboratory of Systems ...
ORGANISM(S): Mus musculus (Mouse) 
2014-07-22 | PXD000471 | Pride
Lc-msms Mouse Osteoclast Triple silac 
ProteomicsMacrophage Proteomic Profiling Reveals Divergent TLR4-Dependent and -Independent Responses to Kdo₂-Lipid A and Lipid IVa
Immortalized mouse macrophages from wild type (WT) were treated with Kdo₂-Lipid A and Lipid IVa under three experimental conditions: (i) extracellular stimulation to assess TLR4-dependent activation with Kdo2 lipid A or lipid-IVa (Extra_Kdo2/Extra_IVa), (ii) direct cytosolic delivery by transfection...
ORGANISM(S): Mus musculus (Mouse) 
2026-08-20 | PXD075293 | Pride
Kdo2-lipid a Lipid iva Tlr4-independent Macrophages Tlr4-dependent Proteomics 
ProteomicsStructural Diversity of Lipid A Modulates Neutrophil Proteome and Secretome Responses
We applied quantitative high-resolution proteomics to systematically evaluate how the human PMN proteome is shaped in response to two LPS preparations that differ primarily in the number of acyl chains associated with their lipid A (E. coli and F. tularensis LPS). To capture both stimulus-specific e...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD072780 | Pride
Lps Francisella tularensis Escherichia coli Neutrophils Secretome 
ProteomicsPhosphoproteome profiling of toll-like receptor response to different ligand stimulation in macrophages identifies differences in global phosphorylation dynamics
Toll-like receptors are among the first sensors that detect and drive immune responses to pathogens. Macrophages that encounter a pathogen are usually not stimulated through one TLR but by a combination of these receptors engaged by distinct ligands produced by the microbe. As a first step to unders...
ORGANISM(S): Mus musculus (Mouse) 
2014-06-09 | PXD000761 | Pride
Tlr4 Macrophage Tlr2 Mouse Tlr7 Phosphoproteomics 
ProteomicsPhosphoproteomic profiling of lipopolysaccharide stimulated toll-like receptor pathways in immortalized mouse macrophages
Toll-like receptors (TLRs) are present on the membranes of immune cells such as dendritic cells, macrophages, and natural killer cells. It is essential for the recognition of microbiological components. They trigger both innate and adaptive immune responses against pathogenic microorganisms. Among T...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-30 | PXD064064 | Pride
Tlr4 Lps Data independent acquisition Phosphoproteomics 
ProteomicsGerm-free mouse ileum iTRAQ shotgun LC-MSMS
Shotgun proteomics of normal versus germ-free mouse terminal ileum. 39 mice. Male and female. BALBc and C57BL10. The samples were labeled using iTRAQ 8plex and analyzed using a nano-LC-LTQ-Orbitrap-Velos-MSMS. This is the proteomics data from an n-omics project.
ORGANISM(S): Mus musculus (Mouse) 
2017-10-04 | PXD003177 | Pride
Lc-msms Germ-free Mouse Itraq Orbitrap Ileum 
ProteomicsProteome-wide Analysis of Palmitoylated Proteins in Macrophages Reveals Novel Insights into Early Immune Signaling
To understand protein S-palmitoylation's role in macrophage reprogramming, dynamic changes were comprehensively mapped in immortalized mouse macrophages during early LPS stimulation using acyl-biotin exchange (ABE) proteomics enhanced with multi-protease digestion. This approach identified 2613 puta...
ORGANISM(S): Mus musculus (Mouse) 
2026-08-20 | PXD063463 | Pride
Macrophage Acyl-biotin exchange (abe) Palmitoylation Immune signaling S-acylation Post-translational modification Lipopolysaccharide (lps) Multi-protease digestion Proteomics 
ProteomicsProteome and Secretome Analysis Reveals Differential Post-Transcriptional Regulation of Toll-Like Receptor Responses
Toll-like receptors (TLRs) are essential sensors in innate immunity and among the first to detect invading pathogens. Many studies of TLR responses have focused on the intracellular signaling events that occur upon receptor stimulation. However, proteins released from the cell play a key role in cel...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-06 | PXD004113 | Pride
Macrophage Lc-msms Raw264.7 Mouse Tlr Secretome 3plex silac Targeted ms 
ProteomicsLPS Time Course stimulation of WT and Δ MARCKS Immortalrized mouse macrophages
WT immortorized mouse macrophages and CISPR CAS 9 mediated Myristoylated, Alanine-rich C-kinase Substrate knockout ΔMARCKS were treated with LPS for 1h, 6h, 24h. No treatment both WT and ΔMARCKS were use as a control experiment. The label free quantification were prefromed using
ORGANISM(S): Mus musculus (Mouse) 
2023-11-20 | PXD042097 | Pride
Macrophage Marcks Lps 
ProteomicsGlobal immunoproteomic profiling of endotoxin-stimulated macrophages uncovers specifics of TLR4- and Caspase 11- mediated recognition
To study innate immunities, secretome was used for quantitative proteomics
ORGANISM(S): Mus musculus (Mouse) 
2024-02-07 | PXD024036 | Pride
Lc-ms Proteomics Silac Lfq Mouse 
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