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ProteomicsDissection of IL-2 and IL-15 signaling pathways
The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we c...
ORGANISM(S): Homo sapiens (Human) 
2014-09-23 | PXD001129 | Pride
Silac Phosphotyrosine Signaling Il-2 Il-15 
ProteomicsAnalysis of E2F2 interacting proteins
In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins...
ORGANISM(S): Homo sapiens (Human) 
2013-01-18 | PXD000026 | Pride
E2f2 Immunoprecipitation Lc-ms/ms 
ProteomicsHERC2 orchestrates assembly quality control of multimeric protein complexes via ubiquitylation of orphan subunits
Most eukaryotic proteins function as subunits within multimeric complexes. Imbalances in subunit levels disrupt assembly, leaving orphan proteins that are degraded by unclear quality control mechanisms. We identify the ubiquitin ligase HERC2 as a key regulator of this process. Proteomic analysis rev...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD071427 | Pride
Assembly quality control Multi-protein complexes Protein stability Ligase Bioub. Herc Proteasome Ubiquitin 
ProteomicsDissection of IL-2 and IL-15 signaling pathways
The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we c...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080767 | MassIVE
ProteomicsChanges in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes
Interleukin-2 (IL-2) stimulation results in T-cell growth as a consequence of activation of highly sophisticated and fine-tuned signaling pathways. Despite lacking intrinsic enzymatic activity, scaffold proteins such as Gab2, play a pivotal role in IL-2-triggered signal transduction integrating, div...
ORGANISM(S): Homo sapiens (Human) 
2016-04-11 | PXD003286 | Pride
Scaffold protein T-lymphocytes Phosphorylation Il-2 Gab2 Interactome 
ProteomicsCharacterization of receptor-associated protein complex assembly in Interleukin (IL)-2- and IL-15-activated T-lymphocytes
Despite extensive investigation, it remains a paradox that IL-2 and IL-15 can differentially modulate the immune response using the same signaling receptors. In our previous work, we dissected the phosphotyrosine-driven signaling cascades triggered by both cytokines in Kit225 T-cells unveiling subtl...
ORGANISM(S): Homo sapiens (Human) 
2016-08-08 | PXD002386 | Pride
Phosphorylation Il-2 Receptor complex assembly Il-15 Interactome 
ProteomicsNuclear phosphoproteomics analysis of resting and IL-2-stimulated T lymphocytes
The aim of the present project was to study in detail the site-specific phosphorylation events occurring both in resting as well as in T lymphocytes stimulated with IL-2 for five minutes. For that purpose we combined SILAC-based quantitative mass spectrometry analysis with phosphopeptide enrichment ...
ORGANISM(S): Homo sapiens (Human) 
2016-04-18 | PXD002839 | Pride
Phosphorylation T-lymphocyte Nucleus Il-2 
ProteomicsFbxo42 promotes the degradation of Ataxin-2 granules to trigger terminal Xbp1 signaling
In Drosophila melanogaster, Fbxo42 acts downstream of Unfolded Protein Response activation but upstream of the apoptotic machinery. Fbxo42 associates through its N-terminal F-box domain with SkpA, a component of the RBX-SCF E3 ubiquitin ligase complex. Hence, in order to uncover Fbxo42 substrates, w...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2025-07-01 | PXD065621 | Pride
Alaxin-2 Upr Fbxo42 Xbp1 
ProteomicsAnalysis of E2F2 interacting proteins
In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-09 | MSV000079567 | MassIVE
ProteomicsCharacterization of receptor-associated protein complex assembly in Interleukin (IL)-2- and IL-15-activated T-lymphocytes
Despite extensive investigation, it remains a paradox that IL-2 and IL-15 can differentially modulate the immune response using the same signaling receptors. In our previous work, we dissected the phosphotyrosine-driven signaling cascades triggered by both cytokines in Kit225 T-cells unveiling subtl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080850 | MassIVE
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