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ProteomicsAnalysis of the interactome of the RLTPR/CARMIL2 protein in CD4+ T cells
We analyzed the interactome of the mouse protein RLTPR (CARMIL2) using an affinity purification method based on the introduction of a One-Strep-tag (OST) sequence in the C-terminus of the bait protein by genetic engineering of primary mouse T cells. We used mouse models expressing the tagged version...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-12 | PXD055893 | Pride
Ap-ms Primary t cell Rltpr Interactome Ost tag Carmil2 
ProteomicsAnalysis of the interactome of LAT in CRISPR/CAS9 modified mouse CD+ T cells
Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the LAT protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around LAT prior to and followin...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018526 | Pride
Ap-ms Tcr Crispr/cas9 Interactome Primary cd+ t cell Ost tag 
ProteomicsAnalysis of the interactome of CD6 in CRISPR/CAS9 modified mouse CD4+ T cells
Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the CD6 protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around the tagged protein prior t...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018527 | Pride
Ap-ms Tcr Crispr/cas9 Primary t cell Interactome Ost tag 
ProteomicsAAnalysis of LAT, SLP76, VAV1 and ZAP70 interactomes of expanded human CD4+ and CD8+ T cells nalysis of LAT, SLP76, VAV1 and ZAP70 interactomes of expanded human CD4+ and CD8+ T cells
Using a CRISPR/Cas9-based approach, we engineered human primary CD4+ and CD8+ T cells in which a bait protein (LAT, SLP76, VAV1 or ZAP70) was tagged with an affinity Twin-Strep-tag (OST), with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosom...
ORGANISM(S): Homo sapiens (Human) 
2022-01-10 | PXD024820 | Pride
Ap-ms Tcr Primary cd4+ and cd8+ human t cell Crispr/cas9 Interactome Ost tag 
ProteomicsAnalysis of SLP-76 interactomes of human primary CD4+ T cells in presence or absence of Dasatinib treatmentAnalysis of SLP-76 interactomes of human primary CD4+ T cells in presence or absence of Dasatinib treatment
Using a CRISPR/Cas9-based approach, we engineered human primary CD4+ T cells in which the bait protein SLP76 was tagged with an affinity Twin-Strep-tag (OST). Through affinity purification coupled with quantitative mass spectrometry, this system allows determining the composition and dynamics of the...
ORGANISM(S): Homo sapiens (Human) 
2022-01-28 | PXD028939 | Pride
Ap-ms Tcr Dasatinib Slp76 Primary cd4+ human t cell Crispr/cas9 Interactome Ost tag 
ProteomicsAnalysis of CD2 interactome in primary murine CD4+ T cells
CD2 is a receptor expressed on T cells and when engaged, it promotes T cell activation. However the mechanisms and molecular partners of CD2 that mediate T cell activation remain unclear. Here we used AP-MS to determine the interactome of CD2 prior and upon its engagement. For this purpose, we used ...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-12 | PXD029750 | Pride
Cd2 Ap-ms Mouse Primary cd4+ t cell Interactome Ost tag 
ProteomicsAnalysis of CD5 interactome in primary mouse CD4+ T cells
CD4+ T cells were isolated from gene-targeted mouse expressing a One-Strep-tag (OST) at the C terminus of the CD5 protein, briefly expanded in vitro and we analyzed the interactome of their OST-tagged CD5 protein. Affinity purification of the OST tagged protein was performed using Streptactin beads,...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018552 | Pride
Ap-ms Tcr Primary cd4+ t cell Interactome Signalosome 
ProteomicsAnalysis of the TCR signalosome in primary T cells
T-cell receptor (TCR) signaling is essential for the function of T cells. Here we combine mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the composition and dynamics of the signaling complexes that assemble around 15 nodes of the TCR signaling cascade o...
ORGANISM(S): Mus musculus (Mouse) 
2019-09-26 | PXD007660 | Pride
Ap-ms Tcr Primary t cell Interactome Signalosome 
ProteomicsAnalysis of the interactomes of ZAP70, CD247 and SLP76 upon stimulation of murine CD8+ T cells with ligands of different affinity to the TCR
T cells have the remarkable ability to sense and discriminate between a wide range of antigenic peptides bound to major histocompatibility complex molecules (pMHC). Here, we use interactomics to monitor in a time dependent manner the early molecular events taking place upon stimulation of murine CD8...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-05 | PXD029974 | Pride
Ap-ms Tcr Slp76 Cd3z Primary cd8+ t cell Ot-i mouse Zap70 Interactome Cd247 Ost tag 
ProteomicsSLP76 interactome in resting and activated primary mast cells
We used a knock-in (KI) mouse expressing SLP-76 with a C-terminal One-Strep-Tag (OST). Such a system allowed the replacement in the genome of SLP-76 by its tagged version and its expression in physiological amounts. Then, by combining high affinity purification and label-free quantitative proteomics...
ORGANISM(S): Mus musculus (Mouse) 
2013-08-28 | PXD000052 | Pride
Interactomics Slp76 Quantitative mass spectrometry 
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