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UnknownThe PRISMA 2020 statement: An updated guideline for reporting systematic reviews.
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S-EPMC8007028 | biostudies-literature
ProteomicsIsolation of the Buchnera aphidicola flagellum basal body complexes from the Buchnera membrane
Buchnera aphidicola is an intracellular bacterial symbiont of aphids and maintains a small genome of only 600 kbps. Buchnera is thought to maintain only genes relevant to the symbiosis with its aphid host. Curiously, the Buchnera genome contains gene clusters coding for flagellum basal body structur...
ORGANISM(S): Buchnera aphidicola str. APS (Acyrthosiphon pisum) 
2021-09-10 | PXD024664 | Pride
Basal body Endosymbiont Buchnera Flagella 
ProteomicsNEDD8 subtrates from NEDP1 knockout cells
The aim of the study was to identify proteins that are modified by NEDD8. The NEDD8 specific protease NEDP1/SENP8 was deleted from HEK 293 cells via Crispr/CAS9 gene editing to allow for the accumulation of NEDD8 modified proteins. Lysates from NEDP1 KO cells were then enriched via pulldown with a...
ORGANISM(S): Homo sapiens (Human) 
2019-02-20 | PXD011928 | Pride
Human Nedd8 Nedp1 Senp8 Hek 293 
ProteomicsDAPK3 modulates migration and invasion of triple negative breast cancers
A mass spectrometry (MS)-based kinase inhibitor pulldown assay (KIPA) was applied to analyze 16 patient-derived xenografts (PDXs) leading to the discovery that Death-Associated Protein Kinase 3 (DAPK3) is significantly and specifically overexpressed in triple negative breast cancer (TNBC). Validatio...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD049187 | Pride
Luzp1 Emt Breast cancer Cancer Dapk3 Pdx 
ProteomicsProtein kinase A phosphorylation of perilipin 5 on serine 155 regulates liver lipolysis via interactions with adipose triglyceride lipase
Perilipin (PLIN) 5 is a lipid droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). We detected phosphorylation on S155, S161 and S163 of recombinant PLIN5 ...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-11 | PXD021969 | Pride
Lipid droplets Fatty acid Perilipin Lipase Triglycerides Lipid metabolism Phosphorylation 
ProteomicsCalmodulin and CHK2 kinase crosslinking
Recombinant proteins were mixed and then crosslinked either with DMTMM or by SDA and UV exposure. Complexes were resolved by SDS-PAGE, excised and tryptic peptides identified by MS.
ORGANISM(S): Homo Sapiens (human) 
2026-03-28 | PXD062332 | JPOST Repository
ProteomicsProximity biotinylation of CDK1 associated proteins in U2OS cells
BirA ligase tagged CDK1 and BirA alone were expressed in U2OS cells. Proximal proteins to CDK1 were labelled by incubating cells with Biotin for 24 hours and biotinylated proteins extracted using Streptavidin pulldown.
ORGANISM(S): Homo sapiens (Human) 
2021-05-12 | PXD024796 | Pride
Human Lc-msms Proximity biotinylation Cdk1 
ProteomicsHeLa and U2OS long term culture adhesion isolations
Integrin adhesion complexes have been previously isolated and analysed by mass-spectrometry from cells largely spread on matrix proteins over a couple of hours. Here we wished to determine the composition of adhesion complexes from cells cultured for 72 hours under standard cell culture conditions a...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD008645 | Pride
Human Cancer cell Proteomics Integrin Adhesion complex Cell culture 
ProteomicsU2OS Adhesion Isolations +/- CytochalasinD and Neomycin
U2OS cells preferentially utilise integrin alphaV beta5 in adhesion complexes when grown under standard cell culture conditions for 3 days. AlphaV beta5 can be found in both classical 'Focal' adhesions and in novel 'Reticular' adhesions that do not associate with F-actin or other known adhesion prot...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD008680 | Pride
Human Cytod Reticular U2os Proteomics 
GenomicsGenome-wide mapping of meiotic double-strand breaks by sequencing Spo11 oligos.
The nonrandom distribution of meiotic recombination shapes patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in accumulation of Spo11 protei...
ORGANISM(S): Saccharomyces cerevisiae 
2011-03-07 | E-GEOD-26449 | biostudies-arrayexpress
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