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ProteomicsDefining proximity proteomics of Histone modifications by antibody-mediated protein A-APEX2 labeling
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein complexes and protein–protein interactions. However, current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins su...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-12 | PXD028063 | Pride
Proximity labeling  post-translationally  amapex  modified histones 
UnknownDefining Proximity Proteome of Histone Modifications by Antibody-mediated Protein A-APEX2 Labeling.
Not available
S-EPMC9510856 | biostudies-literature
ProteomicsProximity labeling by a recombinant APEX2-FGF1 fusion protein reveals interaction of FGF1 with the proteoglycans CD44 and CSPG4
Fibroblast growth factor 1 (FGF1) binds to specific FGF receptors (FGFRs) at the surface of target cells to initiate intracellular signaling. In addition, FGF1 also binds to heparan sulfate proteoglycans (HSPG), which act as important co-receptors. Even if some interactions with HSPGs have been char...
ORGANISM(S): Homo sapiens (Human) 
2018-06-06 | PXD009302 | Pride
Proteomics Proximity labeling Fgf1 
UnknownThe cysteine-free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications.
Not available
S-EPMC6699085 | biostudies-literature
UnknownIn vivo Proximity Labeling of Nuclear and Nucleolar Proteins by a Stably Expressed, DNA Damage-Responsive NONO-APEX2 Fusion Protein.
Not available
S-EPMC9207311 | biostudies-literature
ProteomicsA H2O2-Free Proximity Proteomics Technology for Exploring Dynamic Protein Complexes in Living Systems
Dynamic protein complex assembly is critical for regulating various biological processes. Proximity labeling(PL), best represented by the ascorbate peroxidase APEX2, allows these molecular events to be captured in living cells in a spatiotemporal manner. However, the hydrogen peroxide (H2O2) depende...
ORGANISM(S): Homo sapiens (Human) 
2026-04-14 | PXD064195 | Pride
Ascorbate peroxidase Tyrosine radical H2o2-free proximity labeling 
ProteomicsAPEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
Fip200 Mitophagy Autophagy Frb-rapalog-fkbp interaction Ulk1 Apex2 Er 
ProteomicsProximity labelling identification of ULK1 wild-type (WT) and ULK1-K46I (kinase dead) interactors upon mitochondria tethering in U2OS WT cells
This project involved generating three stable cell lines using a Flp-In system, each containing specific integrated constructs: "FKBPx2-myc-APEX2-ULK1", "FKBPx2-myc-APEX2-ULK1-K46I" (kinase-dead mutant), and "FKBPx2-myc-APEX2" (control). All cell lines additionally express an outer mitochondrial mem...
ORGANISM(S): Homo sapiens (Human) 
2026-04-24 | PXD068381 | Pride
Mitophagy Autophagy Ulk1-k46i Ulk1 Proximity labeling Apex2 
Proteomics4 RABs proxymity labelling - APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting
APEX2-mediated proximity labeling was performed with 4 endosomal RABs allowed an efficient and comprehensive identification of their protein neighbors. APEX2-mediated proximity labeling was performed in triplicate. Protein enrichments obtained from all RABs were compared to APEX2-nude proximity labe...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD010950 | Pride
Early endosomes Apex2 Rabs Membrane trafficking 
ProteomicsC-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-07 | PXD009216 | Pride
Apex2 Biotinylation Centromere Proteomes Cas9 Subnuclear Telomere 
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