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ProteomicsProtein Corona of 60nm Silver Nanoparticles
We studied the composition of the protein corona formed on 60 nm silver nanoparticles with citrate coating interacted with the human blood plasma under various pH and temperature conditions. The protein corona was analyzed using LC-MS and label-free quantitation.
ORGANISM(S): Homo sapiens (Human) 
2019-01-23 | PXD007648 | Pride
Ag Lfq Nanoparticle Protein corona 
ProteomicsHigh-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry
A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hun...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-08-04 | PXD063043 | Pride
Nanoparticle protein corona Bottom-up proteomics High-throughput Cze-ms/ms Biomarker 
ProteomicsConcentration and composition of the protein corona as a function of incubation time and serum concentration
We describe the concentration and composition of the protein corona as a function of incubation time (30 min and 1 day) and the concentration of fetal bovine serum (FBS) used to form the corona (5%, 10%, and 100%) on 200 nm carboxylate-modified magnetic NPs, using semi-automated processes.
ORGANISM(S): Bos taurus (Bovine) 
2023-03-21 | PXD035811 | Pride
Bovine Serum Magnetic nanoparticles Protein corona 
ProteomicsPlasma protein corona on superparamagnetic iron oxide nanoparticles (SPIONs)
We determined the composition of the plasma protein corona on silica-coated versus dextran-coated iron oxide nanoparticles using mass spectrometry-based proteomics approaches. Gene ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA) revealed distinct protein corona compositions fo...
ORGANISM(S): Homo sapiens (Human) 
2015-10-12 | PXD000766 | Pride
Protein corona Superparamagnetic Nanoparticles Mass spectrometry Macrophages 
ProteomicsNew method to investigate protein orientation in corona of nanomaterials
The composition and structure of protein corona represents a crucial factor that controls the biological properties and fate of nanomaterials. While the composition of the corona can relatively easily be investigated by conventional bottom-up proteomics, assessing the spatial orientation of a protei...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019524 | Pride
Isobaric labeling Orientation Protein corona 
ProteomicsMass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona
Conventional mass spectrometry (MS)-based bottom-up proteomics (BUP) analysis of protein corona [i.e., an evolving layer of biomolecules, mostly proteins, formed on the surface of nanoparticles (NPs) during their interactions with biomolecular fluids] enabled nanomedicine community to partly identif...
ORGANISM(S): Homo sapiens (Human) 
2024-10-15 | PXD050779 | Pride
Protein corona Top-down proteomics Nanomedicine Plasma Proteoform 
ProteomicsIn situ Analysis of Nanoparticle Soft Corona and Dynamic Evolution
How soft corona i.e., the protein corona’s outer layer contributes to “biological” identity of nanomaterials (NMs) is largely unknown due to a longstanding challenge in capturing protein composition of the soft corona in situ. We herein develop an in situ “Fishing” method that can monitor the dynami...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD033976 | Pride
Protein corona Mouse serum 
ProteomicsIntegrated top-down and bottom-up mass spectrometry enables precise characterization of proteoforms and their post-translational modifications within the protein corona
Precise characterization of proteins and proteoforms within the protein corona is essential for developing safer and more effective nanomedicines for diagnostic and therapeutic applications. Although the protein corona phenomenon has been recognized in nanomedicine for nearly two decades, the applic...
ORGANISM(S): Homo sapiens (Human) 
2026-05-14 | PXD077545 | Pride
Bup Peptide Ptm Proteoform Protein corona Tdp 
ProteomicsDeep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules
The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as a valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show that spiking pho...
ORGANISM(S): Homo sapiens (Human) 
2024-10-15 | PXD053359 | Pride
Protein corona Top down proteomics Human plasma Bottom up proteomics Nanoparticles 
ProteomicsMass Spectrometry-Based Top-Down Proteomics for Proteoform Profiling of Protein Corona
The protein corona, a layer of biomolecules—primarily proteins—that adsorb to nanoparticle (NP) surfaces in biological fluids, critically affects NP safety and their therapeutic and diagnostic functions. Additionally, the protein corona effectively reduces proteome complexity and the dynamic range o...
ORGANISM(S): Homo sapiens (Human) 
2025-09-08 | PXD060784 | Pride
Top-down proteomics Mass spectrometry Nanomedicine Protein corona 
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