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ProteomicsThe specific localization and function of Cdc42 isoforms
Cdc42 is a small GTPase protein whose role in polarity is widely studied. However, in vertebrates there exist 2 isoforms arising due to alternative splicing. The function and localization of these have not been studied with respect to cell polarity and cell migration. We therefore perform a mass spe...
ORGANISM(S): Homo sapiens (Human) 
2024-01-23 | PXD017477 | Pride
Golgi Isoforms Sub-cellular localization Cdc42 
ProteomicsSILAKin: A novel high throughput SILAC-based quantitative mass spectrometry assay to identify the substratome of kinases secreted by pathogens
We developed SILAkin, a novel and easy method to identify substrates that is applicable to most kinases. It combines phosphatase treatment, pulse heating, in vitro kinase assay and SILAC (Stable Isotope Labeling with Amino acids in Cell culture)-based quantitative mass spectrometry (MS). SILAkin was...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026220 | Pride
Casein kinase 1 Host-pathogen interactions Substrate screen Leishmania 
ProteomicsTranslation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis
In the Caenorhabditis elegans germline, thousands of mRNAs are concomitantly expressed with antisense 22G-RNAs, which are loaded into the Argonaute CSR-1. Despite their essential functions for animal fertility and embryonic development, how CSR-1 22G-RNAs are produced remains unknown. Here, we show ...
ORGANISM(S): Caenorhabditis elegans 
2021-04-23 | PXD020293 | Pride
Csr-1 Argonaute C. elegans Small rna 
ProteomicsNetwork-based integration of multi-omics data identifies the determinants of miR-491-5p effects
The identification of miRNAs’ targets and associated regulatory networks might allow the definition of new strategies using drugs whose association might mimic a given miRNA’s effects. Based on this assumption our group devised a multi-omics approach in an attempt to precisely characterize miRNAs’ e...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022131 | Pride
Ovarian cancer Mirna Network Multi-omics 
ProteomicsAdult mouse choroid plexus OTX2 Co-IP
To analyze OTX2 function in adult choroid plexus, we performed several OTX2 co-immunoprecipitation (co-IP) experiments with mass spectrometry analysis to identify potential protein partners. We previously discovered that OTX2 protein also accumulates non-cell autonomously in subventricular zone (SVZ...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-23 | PXD021244 | Pride
Homeoprotein Mouse Otx2 Choroid plexus 
ProteomicsProteomic analysis of the secretome from 4T1 breast cancer cells upon Stat3 silencing.
Stat3 has been acknowledged as an oncogene that has dual protumorigenic activity in a cell-intrinsic way, by promoting cancer cell proliferation and survival and in a paracrine mode, acting as suppressor of immune cell attack. We demonstrated that Stat3 silencing with a small interfering RNA (siRNA)...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-21 | PXD013948 | Pride
4t1 Stat3 Secretome 
ProteomicsConsequences of SF3B1 mutation on the proteome
In this study, we evaluated the differential proteome of SF3B1-mutated and SF3B1-Wild-Type uveal melanoma cells (isogenic Mel202 cells). We performed a quantitative label-free tandem liquid chromatography mass spectrometry (LC-MS/MS) analysis of SF3B1WT and SF3B1mut Mel202 cells.
ORGANISM(S): Homo sapiens (Human) 
2022-03-15 | PXD022726 | Pride
Cancer Uveal melanoma Sf3b1 Proteome Mutation 
ProteomicsNucleolin uses its RBD2 domain to recognize long-loop G-Quadruplexes from human minisatellite CEB25
We investigated herein the interaction between nucleolin (NCL) and a set of G4 sequences derived from the CEB25 human minisatellite which adopt a parallel topology while differing by the length of the central loop (from 9nt to1nt). It is revealed that NCL strongly binds to long-loop (9-5 nt) G4 whil...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016529 | Pride
Rna binding domain G4-ligands Proteomics G-quadruplex Rgg domain Photo-crosslinking Nucleolin Long loop 
ProteomicsGalectins expression profile in mice intestine - Galectin 3 interacting partners identification
In this present project, we aimed to physiologically dissect the contribution of glycolipid- and lectin-driven endocytosis mechanism that operate within the mice intestine. We therefore decided to first characterize the expression profile of galectins in this tissue and then to focus on the specific...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-07 | PXD016499 | Pride
Intestine Galectins Mice 
ProteomicsSPEN is a molecular integrator that bridges Xist with transcriptional and epigenetic control of X-chromosome inactivation
Xist represents a paradigm for long non-coding RNA function in epigenetic regulation, although how it mediates X-chromosome inactivation (XCI) remains largely unexplained. Multiple Xist-RNA binding proteins have recently been identified, including SPEN/SHARP, whose knockdown has been associated with...
ORGANISM(S): Mus musculus (Mouse) 
2021-04-19 | PXD015699 | Pride
Xci X-chromosome inactivation Epigenetic Xist Spen 
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