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ProteomicsComprehensive chemical proteomics for target deconvolution of the redox active drug auranofin
Chemical proteomics encompasses novel drug target deconvolution methods in which compound modification is not required. Herein we use Thermal Proteome Profiling, Functional Identification of Target by Expression Proteomics and multiplexed redox proteomics for deconvolution of auranofin targets to ai...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016776 | Pride
Cancer Nfkb2 Mass spectrometry Hct116 Thioredoxin reductase Expression Chordc1 Database A375 Drug Proteomics Resource Rko Chemotherapeutic Auranofin Compound 
ProteomicsA PTM-centric Proteome Informatic Pipeline for Monitoring Post-Translational Modifications by PEIMAN2
Post-translational modifications (PTMs) are under significant focus in molecular biomedicine due to their importance in signal transduction in most cellular and organismal processes. Identification of PTMs, determination of PTM location sites, discrimination between functional and inert PTMs, and qu...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD037681 | Pride
Ptm Proteomics Mass spectromtery 
ProteomicsA PTM-centric Proteome Informatic Pipeline for Monitoring Post-Translational Modifications by PEIMAN2
Post-translational modifications (PTMs) are under significant focus in molecular biomedicine due to their importance in signal transduction in most cellular and organismal processes. Identification of PTMs, determination of PTM location sites, discrimination between functional and inert PTMs, and qu...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD037679 | Pride
Mass spectrometry Ptm Proteomics Bioinformatics 
ProteomicsNCF1-dependent low production of reactive oxygen species in plasmacytoid dendritic cells exacerbates lupus
Low capacity to produce reactive oxygen species (ROS) due to mutations in neutrophil cytosolic factor 1 (NCF1/p47phox) is strongly associated with lupus development both in humans and mouse models. Here, we aim to identify the major mechanisms of the Ncf1-disease association. We found that plasmacyt...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-12 | PXD035964 | Pride
Lupus Reactive oxygen species Plasmacytoid dendritic cells 
ProteomicsComparative proteomics of dying and surviving cancer cells improves the identification of drug targets and sheds light on cell life/death decisions
Chemotherapeutics cause the detachment and death of adherent cancer cells. When studying the proteome changes to determine the protein target and mechanism of action of anticancer drugs, the still-attached cells are normally used, while the detached cells are usually ignored. Here we tested the hypo...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD007686 | Pride
Cell death Mass spectrometry Survival Drug mechanism Chemotherapeutic 
ProteomicsProTargetMiner: A proteome library of anticancer molecules for functional discovery
ProTargetMiner is the first publicly available expandable proteome signature library of anticancer molecules in cancer cell lines. The compounds cluster into groups by their proteomic signatures based on their targets and mechanisms. These can be deconvoluted by orthogonal partial least square (OPLS...
ORGANISM(S): Homo sapiens (Human) 
2019-11-01 | PXD013134 | Pride
Cancer Drug Proteomics Mass spectrometry Mcf-7 Resource Rko Expression Chemotherapeutic Database Compound 
ProteomicsProTargetMiner: A proteome library of anticancer molecules for functional discovery
As a further development of our Functional Identification of drug Targets by Expression Proteomics (FITExP) method, a reference dataset was built of the proteomic responses of A549 human adenocarcinoma cells to 56 clinical and experimental anticancer molecules. For each molecule, a concentration was...
ORGANISM(S): Homo sapiens (Human) 
2019-11-01 | PXD009775 | Pride
Cancer Drug Proteomics Mass spectrometry A549 Resource Expression Chemotherapeutic Database Compound 
ProteomicsProTargetMiner: A proteome library of anticancer molecules for functional discovery
As a further development of our Functional Identification of drug Targets by Expression Proteomics (FITExP) method, a reference dataset was built of the proteomic responses of A549 human adenocarcinoma cells to 56 clinical and experimental anticancer molecules. For each molecule, a concentration was...
ORGANISM(S): Homo sapiens (Human) 
2019-11-01 | PXD009644 | Pride
Cancer Drug Proteomics Mass spectrometry A549 Resource Expression Chemotherapeutic Database Compound 
ProteomicsRedox regulation of PTPN22 affects the severity of T cell dependent autoimmune inflammation
PTPN22, a modifier of T cell receptor signaling, and reactive oxygen species are major players in the regulation of chronic autoimmune diseases. As with all protein tyrosine phosphatases the activity of PTPN22 is redox regulated, but if or how such regulation can modulate inflammatory pathways in vi...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-23 | PXD025319 | Pride
Autoimmunity Ptpn22 Reactive oxygen species Lymphocyte signaling 
ProteomicsSystem-wide identification of enzyme substrates by thermal analysis (SIESTA)
Despite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Th...
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD014445 | Pride
Proteomics Post-translational modification Target Thermal stability Lc/ms Akt1 Trxr1 Parp10 Artd10 Rac-alpha serine threonine protein kinase Txnrd1 
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