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ProteomicsHepG2 GalNAc-T1/2 Bump-and-hole secretome glycopeptide data
Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAC analog into the secretome of HepG2 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-azide. Glycoproteins were...
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD018048 | Pride
Human Bioorthogonal Galnac-t Liver Glycopeptide Click Secretome Hepg2 
ProteomicsK-562 GalNAc-T1/2 Bump-and-hole Whole cell lysate proteomics data
Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAc analog into the glycoproteins on the cell surface of K-562 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-a...
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD017989 | Pride
Human Mucin Bioorthogonal Galnac-t Click Glycoprotein K-562 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035438 | Pride
Mcf7 Co-culture  lfq Proteomics Human-murine 4t1 Secretome 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035449 | Pride
Mcf7 Human-murine Lysate 4t1 Co-culture  glycoproteomics 
ProteomicsCell-type specific tagging of glycoproteins in murine-human co-culture secretome glycoproteomics
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected ce...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-10 | PXD035445 | Pride
Mcf7 Human-murine 4t1 Secretome Co-culture  glycoproteomics 
ProteomicsCell-type specific labelling of O-linked glycoproteins in engineered K-562 cells
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035437 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsCell-type specific labelling of glycoproteins in engineered K-562 cells (BOCTAG)
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035430 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsHepG2 secretome tagged with the sugar analogs GalNAz and GalNAzMe
Chemically modified analogs of GalNAc, termed GalNAz or GalNAzMe, were incorporated in secreted glycoproteins. The compounds contain bioorthogonal, clickable functional groups that allowed for enrichment using biotin. Chemical glycoproteomics was performed after on-bead digestion and cleavage of gly...
ORGANISM(S): Homo sapiens (Human) 
2020-09-14 | PXD020648 | Pride
Glycosylation Bioorthogonal Mucin Chemical proteomics Enrichment Biotin 
UnknownOccludin knockdown is not sufficient to induce transepithelial macromolecule passage.
Not available
S-EPMC6619991 | biostudies-literature
UnknownCeliac Disease Monocytes Induce a Barrier Defect in Intestinal Epithelial Cells.
Not available
S-EPMC6888450 | biostudies-literature
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