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ProteomicsMass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants
We acquired quantitative proteomics data from eight week old ppi2 and tic56-1 mutants as well as from wild type treated with 60 µg/ml spectinomycin and related them to their respective wildtype reference. Protein identification and quantification was performed by data-independent MSE acquisition on ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-14 | PXD004125 | Pride
Hdmse Tic56-1 Plastid Spectinomycin Plastidic translation Quantitative mass spectrometry Shotgun proteomics Toc-tic-import machinery Hi3 quantification Ppi2 
ProteomicsIdentifying mTOR regulated RNA binding proteins
To investigate the RNA binding proteins that change following mTOR inhibition, HeLa cells were treated with and without 200nM Torin1 for an hour. Cells were the UV254 cross-linked, lysed and large scale whole cell oligo(dT) based pulldowns were performed with identification and quantification of pu...
ORGANISM(S): Homo sapiens (Human) 
2020-11-24 | PXD016705 | Pride
Nanoflow. Larp1 Mtor Synapt g2s Rna-binding proteins 
UnknownQuantitation of the Noncovalent Cellular Retinol-Binding Protein, Type 1 Complex Through Native Mass Spectrometry.
Not available
S-EPMC5728378 | biostudies-literature
ProteomicsCryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate
Uncovering the molecular architecture of the core FADD:Caspase-8 complex and how this is altered by regulatory partners, such as the cell death inhibitor c-FLIP, is essential to understand co-ordination of cell fate. Here, using electron microscopy, we visualize for the first time fulllength proca...
ORGANISM(S): Homo sapiens (Human) 
2021-02-08 | PXD022408 | Pride
Caspase 8 Cell death. disc Fadd Em C-flip complex Structure 
ProteomicsHeparin_sepharose_A.thaliana - The peptide microarray "ChloroPhos1
Heparin sepharose CL-6B enrichment of lysed Arabidopsis thaliana chloroplasts was done. Elution was performed with 1M (NH4)2SO4. This elution fraction contained plastid casein kinase II activity. Their presence is proven by this multiplex MS data.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-07-08 | PXD000981 | Pride
A thaliana Ck ii Heparin sepharose 
ProteomicsProteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking
Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron ox...
ORGANISM(S): Homo sapiens (Human) 
2014-09-25 | PXD001175 | Pride
Human Hdmse Dia Intracellular trafficking Hela Synapt g2s Nanoparticles Endosomes Vesicles 
ProteomicsEffects of SHetA2 on SiHa and C-33 A human cervical cancer cell lines
The objective was to identify how cervical cancer cells respond to treatment with the investigational new agent, Sulfur Heteroartoinoid A2 (SHetA2, NSC 726189) by identify proteins that are detected at significantly different levels in SHetA2-treated compared to untreated control cultures. Triplicat...
ORGANISM(S): Homo sapiens (Human) 
2023-08-29 | PXD029537 | Pride
Cervical cancer Cancer prevention Drug development Nsc 726189 Sheta2 Cancer therapeutics 
ProteomicsLabel-free quantification in ion-mobility enhanced data-independent acquisition proteomics
Data-dependent acquisition (DDA) methods are a well-established tool for proteome analysis and have greatly expedited the field of shotgun proteomics. However, their serial and stochastic nature restricts their reproducibility and limits the detectable dynamic range to the peptides that ionize best....
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast) Escherichia coli 
2016-03-29 | PXD002393 | Pride
Proteomics Mass spectrometry Label-free quantification Ion mobility separation Data-independent acquisition 
ProteomicsNodule Zone Specific Proteome - Integrated analysis of zone-specific protein and metabolite profiles within nitrogen-fixing Medicago truncatula-Sinorhizobium medicae nodules
Root nodules of the medicago truncatula-sinorhizobium meliloti plant-bacterail symbiotic model system were hand sectioned followed by LCMS. Sections were made to separate different developmental zones along the nodules longitudinal axis.
ORGANISM(S): Sinorhizobium meliloti 1021 Medicago truncatula (Barrel medic) (Medicago tribuloides) 
2017-07-17 | PXD006683 | Pride
Sinorhizobium Truncatula Nitrogen Zone Proteomics Meliloti Symbiotic Fixation Root Medicago Nodule 
ProteomicsProteomic Sample Preparation in the Low Microgram Range – A Critical Evaluation of Current Workflows
Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Over the past years, several protocols been specifically developed for the preparation of samples, which are limited in quantity. In the present study, we carried ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-05-11 | PXD006760 | Pride
Single-pot solid-phase-enhanced sample preparation (sp3) Filter-aided sample preparation (fasp) In-stagetip digestion (ist) Sample preparation Bottom-up proteomics 
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