Sort   by:  
 Page size 
ProteomicsEpisodic Transport of Protein Aggregates Achieves a Positive Size Selectivity in Aggresome Formation
To generate this dataset, different proteins (human HDAC6, CCT8, 26S proteasome, Hsc70, Hsp90, p62, and Xenopus HOOK2 were immunoprecipitated from the Hela S3 cell extract or Xenopus egg extract by the Dynabeads Protein G beads (Thermo Fisher, #10004D) coated with corresponding primary antibodies as...
ORGANISM(S): Homo sapiens (Human) 
2025-07-16 | PXD054601 | Pride
Lc-ms Ip 
ProteomicsIntegrating endometrial proteomic and single cell transcriptomic pipelines reveals distinct menstrual cycle and endometriosis-associated molecular profiles
Endometriosis is a debilitating gynecological disorder affecting approximately 10% of the female population. Despite its prevalence, robust methods to classify and treat endometriosis remain elusive. Changes throughout the menstrual cycle in tissue size, architecture, cellular composition, and indiv...
ORGANISM(S): Homo sapiens (Human) 
2023-09-05 | PXD045115 | Pride
Endometriosis Thermo orbitrap exploris480 Lc-ms/ms 
ProteomicsRB1 mass spec with or without MYC amplification
293T cells were transfected with Flag-tagged RB1 with or without MYC. After 48 h, the cells were lysed with IP buffer and immunoprecipitated with Flag-conjugated agarose beads (Sigma-Aldrich). The bound proteins were eluted with 2% SDS and digested overnight with sequencing grade modified trypsin (P...
ORGANISM(S): Homo Sapiens 
2022-10-17 | PXD037479 |
ProteomicsIn-source CID improves HCD-dependent fragmentation of ADP-ribosyl peptides
ADP-ribosylation is a posttranslational modification whose HCD products are dominated by complete or partial modification losses, complicating peptide sequencing and acceptor site localization efforts. We tested whether in-source CID performed on a quadrupole Orbitrap could convert ADPr to the small...
ORGANISM(S): Homo sapiens (Human) 
2024-12-05 | PXD055586 | Pride
Sid Proteomics Hcd Exploris480 Adp-ribosylation 
ProteomicsSustained PRC1.1 activity preserves gene repression independent of PRC2.2 and suppresses leukemic cell differentiation
Loss-of-function mutations in BCOR, subunit of the non-canonical Polycomb Repressive Complex 1.1 (PRC1.1), are frequently observed in acute myeloid leukemia (AML) and associate with adverse risk, but underlying mechanisms driving leukemogenesis remain elusive. Here, we find that BCOR is a bridging f...
ORGANISM(S): Homo sapiens (Human) 
2026-06-15 | PXD065157 | Pride
Human Exploris480 
ProteomicsccRCC cells treated with DMSO or UNC8209
Clear cell renal cell carcinoma (ccRCC), the most common kidney cancer subtype, often features inactivation of the VHL tumor suppressor, creating therapeutic vulnerabilities. Although HIF2α inhibitors have shown promise, many VHL-deficient tumors remain resistant. TANK-binding kinase 1 (TBK1) has be...
ORGANISM(S): Homo sapiens (Human) 
2026-03-10 | PXD071969 | Pride
Lc-msms Protac Tbk1 Ccrcc 
ProteomicsA Defective Extracellular Matrix/Kindlin-2/Actin/ERK Mechanosensing Pathway Affects Fibroblast-To-Myofibroblast Transition in the Old Male Heart
Heart failure represents a leading cause of mortality in the elderly population. Although aging features include diastolic dysfunction and interstitial fibrosis in both males and females, it becomes increasingly apparent that aged male and female hearts are phenotypically different. There were funda...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-11 | PXD041660 | Pride
Extracellular matrix (ecm) Heart Fibroblast 
ProteomicsDICER1 syndrome-associated mutations lead to 3p-miRNA gain of function and increased metastatic potential
The DICER1 gene is mutated in cancer, including Dicer1 syndrome, a rare tumour predisposition syndrome. Cancer-associated hotspots mutations have been reported in both catalytic domains of Dicer, and are predicted to disrupt miRNA processing activity. To understand these hotspot mutations in cancer ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-23 | PXD072167 | Pride
Cancer Dia Dicer1 Mutations Exploris480 
ProteomicsProteome analysis in hepatocyte-specific Atg7-knockout mice with different p62 mutations
To investigate global proteome changes dependent on KEAP1-p62 binding in vivo, we performed cytoplasmic proteome profiling.
ORGANISM(S): Mus musculus (Mouse) 
2025-04-23 | PXD061879 | Pride
Lc-msms Liver Mice 
ProteomicsProteomic analysis of kidney organoid supernatant during TNFa stimulation
Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome after treatment of organoids with 5ng/mL TNFalpha for 24h and 48h compare...
ORGANISM(S): Homo sapiens (Human) 
2023-05-12 | PXD029696 | Pride
Tnfalpha Cytokine Shotgun lc ms/ms Human kidney organoids Tnfa 
Sort   by:  
 Page size