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ProteomicsNative Purification of yeast Met30 in the absence and presence of Cadmium followed by DSSO crosslinking and Mass spec analysis
HBTHMet30 cells were cultured in YEP + 2% galactose + 4µM Biotin at 30˚C and treated with 100 µM CdCl2 for 30 minutes. Native whole cells lysates were prepared in lysis buffer (50mM Hepes pH7.5, 200mM NaCl, 10% glycerol, 40mM imidazole, 0.2% Triton1mM dithiothreitol, 0.1mM orthovanadate, 1mM phenylm...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-06-22 | PXD048194 | Pride
Scfmet30 Met30 intra-crosslinks Dsso Mass spectrometry Cadmium 
ProteomicsPentatricopeptide repeat poly(A) binding protein from mitochondria of trypanosomes
In Trypanosoma brucei, most mitochondrial mRNAs undergo U-insertion/deletion editing, and 3′ adenylation and uridylation. The internal sequence changes and terminal extensions are coordinated: Pre-editing addition of the short (A) tail protects the edited transcript against 3′-5′ degradation, while ...
ORGANISM(S): Trypanosoma brucei 
2019-04-02 | PXD012008 | Pride
Ppr protein Trypanosoma Rna stability Mitochondria Rna editing Polyadenylation 
ProteomicsLENG8 mediates nuclear retention and degradation of misprocessed mRNAs and noncoding RNAs
Incompletely processed and misprocessed mRNAs as well as numerous noncoding RNAs are retained in the nucleus and often degraded, and defects in this quality control mechanism have been linked to various diseases, including cancer and neurodegeneration. However, the underlying mechanisms remain poorl...
ORGANISM(S): Homo sapiens (Human) 
2026-03-30 | PXD065356 | Pride
Misprocessed rnas Rna nuclear retention Rna degradation Leng8 
ProteomicsTrioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks
Here, we present a novel trioxane-based MS-cleavable homotrifunctional cross-linker TSTO, which can target three proximal lysine residues simultaneously. Owing to its unique structure and MS-cleavability, TSTO enables fast and unambiguous identification of cross-linked peptides using LC-MSn analysis...
ORGANISM(S): Homo sapiens (Human) 
2025-05-30 | PXD054551 | Pride
Tsto Xl-ms Cross-linking mass spectrometry 
ProteomicsTrioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks
Here, we present a novel trioxane-based MS-cleavable homotrifunctional cross-linker TSTO, which can target three proximal lysine residues simultaneously. Owing to its unique structure and MS-cleavability, TSTO enables fast and unambiguous identification of cross-linked peptides using LC-MSn analysis...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-30 | PXD063825 | Pride
Tsto Xl-ms Lc-msn 
ProteomicsHuntingtin contains an ubiquitin-binding domain and regulates lysosomal targeting of mitochondrial and RNA-binding proteins
Evaluating the identity of ubiquitinated and ubiquitin-associated proteins tandem co-purifying non-covalently with wild-type (17 glutamine) human Huntingtin fragment, amino acids 1-502 C-terminally tagged with the HA peptide epitope, basally expressed from the CMV promoter within vector pcDNA3.1 tra...
ORGANISM(S): Homo sapiens (Human) 
2024-07-31 | PXD053980 | Pride
Mitophagy Huntington’s disease Selective autophagy Rna-binding proteins Huntingtin Ubiquitin-binding domain 
ProteomicsStructural analysis of the CSN complex to understand its inhibition mechanism
We performed interaction and structural analyses to unravel the molecular mechanisms underlying CSN inhibition. Various methods including Cryo-EM, quantitative mass spectrometry and biochemical assays have been employed to define inhibitor-induced interaction and structural changes of the CSN comple...
ORGANISM(S): Homo sapiens (Human) 
2025-11-22 | PXD063822 | Pride
Csn Quantitative ms Crls Protein ubiquitination Neddylation Cryo-em Cop9 signalsome 
ProteomicsStructural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing
Structures of RNA editing substrate binding complexes reveal the mechanisms of guide RNA stabilization and messenger RNA recognition in the Trypanosoma brucei mitochondrion.
ORGANISM(S): Trypanosoma brucei 
2024-01-26 | PXD039156 | Pride
Resc Recc Trypanosoma brucei 
ProteomicsExploration of protein degradability enables fully endogenous MrTAC degraders
Methylarginine degrons mark proteins for degradation in lysosomes. We leveraged the machinery responsible for methylarginine degron addition to create a chemically inducible degrader named a Methyl Arginine Targeting Chimera (MrTAC). First, we evaluated the contents of isolated lysosomes following a...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD071805 | Pride
UnknownVitamin D Signaling Suppresses Early Prostate Carcinogenesis in TgAPT121 Mice.
Not available
S-EPMC7194567 | biostudies-literature
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