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ProteomicsQuantitative phosphoproteomics unveils temporal dynamics of thrombin signaling in human endothelial cells
Thrombin is the key serine protease of the coagulation cascade and a potent trigger of protease-activated receptor (PAR)1-mediated platelet aggregation. In recent years, PAR1 has become an appealing target for anticoagulant therapies. However, the inhibitors that have been developed so far increase ...
ORGANISM(S): Homo sapiens (Human) 
2014-02-06 | PXD000597 | Pride
Endothelial cells Silac Thrombin Phosphoproteomics Par Secretome 
ProteomicsE-cadherin interactome complexity and robustness resolved by quantitative proteomics
E-cadherin-mediated cell-cell adhesion and signaling plays an essential role in development and maintenance of healthy epithelial tissues. Adhesiveness mediated by E-cadherin is conferred by its extracellular cadherin domains and is regulated by an assembly of adaptors and enzymes associated with it...
ORGANISM(S): Homo sapiens (Human) 
2016-07-06 | PXD000448 | Pride
E-cadherin  silac  label-free  interactome  cancer 
ProteomicsA proteomic approach to identify endosomal cargoes controlling cancer invasiveness
We have developed a proteomic approach to identify endosomal cargoes that contribute to cancer invasiveness.
ORGANISM(S): Homo sapiens (Human) 
2017-01-18 | PXD003745 | Pride
Silac  label-free  cancer  recyclome  orbitrap 
ProteomicsIn-depth proteomics identifies a role for autophagy in controlling ROS-mediated endothelial barrier permeability
We have used in-depth quantitative proteomics to identify processes required for the formation/maintenance of a stable new vessel. To do that we have used SILAC-based proteomics and a simple in vitro humam endothelial cell model that recapitulates vessel formation in vivo.
ORGANISM(S): Homo sapiens (Human) 
2016-06-07 | PXD003284 | Pride
Autophagy Permeability Silac Endothelial cells 
ProteomicsFormate-induced phospho-signalling
The mitochondrial serine catabolism to formate induces a metabolic switch to a hypermetabolic state with high rates of glycolysis, purine synthesis and pyrimidine synthesis. While formate is a purine precursor it is not obvious link between formate and pyrimidine synthesis. Methods Here we combine p...
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD019347 | Pride
Metabolism Formate Phosphoproteomics Ms Cancer 
ProteomicsSILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers
Abstract: Proteomics has been successfully used for cell culture on dishes, but more complex cellular systems have proven to be challenging and so far poorly approached with proteomics. Because of the complexity of the angiogenic program, we still do not have a complete understanding of the molecula...
ORGANISM(S): Homo sapiens (Human) 
2013-09-04 | PXD000359 | Pride
Angiogenesis Silac Huvecs Lc-ms/ms 
ProteomicsPhosphodiesterase 2A2 (PDE2A2) interactome
Here we provide a label-free interactome of PDE2A2 tagged with a Strep-tag II epitope at the carboxyl terminal in HT-4 mouse neuroblastoma cells.
ORGANISM(S): Mus musculus (Mouse) 
2020-10-12 | PXD020486 | Pride
Pde2a2 Mitophagy Neuroblastoma cells Mitochondria Interactome 
ProteomicsHypoxia-induced proteomic remodeling of human mammary cancer associated fibroblast - siRNA NCBP2-AS2
Intratumoral hypoxia causes the formation of dysfunctional blood vessels which contribute to tumor metastasis. Blood vessels are embedded in the tumor stroma where cancer-associated fibroblasts (CAFs) constitute the most prominent cellular component. We found that hypoxic human mammary CAFs promote ...
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD006539 | Pride
Cancer Angiogenesis Hypoxia Silac Caf Proteome Secretome 
ProteomicsHypoxia-induced proteomic remodeling of human mammary cancer associated fibroblast
Intratumoral hypoxia causes the formation of dysfunctional blood vessels which contribute to tumor metastasis. Blood vessels are embedded in the tumor stroma where cancer-associated fibroblasts (CAFs) constitute the most prominent cellular component. We found that hypoxic human mammary CAFs promote ...
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD006535 | Pride
Breast cancer  caf  proteomics  secretome  hypoxia  angiogenesis 
ProteomicsNeuropilin 1 interactome in endothelial cells
To get unbiased insights on the molecular networks through by which Neuropilin 1 (NRP1) controls endothelial cell (EC) and blood vessel functions independently from its ligands, wehave implemented a Halo Tag (HT)-based approach, which enables effective surface labeling and pull-down of proteins, and...
ORGANISM(S): Homo sapiens (Human) 
2022-06-15 | PXD019700 | Pride
Endothelial cells Vessel permeability Ms-proteomics Neuropilin Interactome Blood vessels Cell-cell adhesion 
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