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ProteomicsThe mitochondrial and chloroplast proteome of Euglena gracilis
Euglena gracilis is regarded as a flexible and adaptable free-living protist of considerable biotechnological interest. We carried out proteomic analysis of the E. gracilis purified chloroplast and mitochondrion. These data provide a new level of insight into functions and unique characteristics of ...
ORGANISM(S): Euglena gracilis 
2019-10-28 | PXD014767 | Pride
Euglena gracilis Mitochondrion Chloroplast 
ProteomicsProbing the trypanosome nuclear pore by TurboID proximity labelling – Part3
Previous BioID experiments targeting the nucleoporin (NUP) NUP158 (PMID: 36410438; PXD031245), as well as NUP110, NUP76, NUP96 (PMID: 39206942; PXD047268)and NUP75, transport factors MEX67 and Ran(PXD055934), indicated that proximity labelling delivers highly specific interactome data in the confine...
ORGANISM(S): Trypanosoma brucei 
2025-05-07 | PXD059554 | Pride
Turboid Proximity labeling Nuclear pore Trypanosoma brucei 
ProteomicsProximity labelling and cryomill affinity purification compared for Trypanosoma brucei proteins with unconfined, semi-confined and confined localisation
Identification of protein-protein interactions is a major contributor for understanding protein function and elucidating molecular and cellular mechanisms. Two strategies are currently popular for identification of protein interaction partners directly from the cellular environment. Affinity purific...
ORGANISM(S): Trypanosoma brucei 
2022-11-30 | PXD031245 | Pride
Cryomilling Affinity purification Proteome Bioid Interactome 
ProteomicsImmunoisolation of the trypanosome decapping enzyme ALPH1 delivers a potential subcomplex
Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2...
ORGANISM(S): Trypanosoma brucei 
2023-06-14 | PXD042322 | Pride
Xrna Mrna decay Apah-like phosphatase Alph1 Mrna decapping Trypanosoma Exoribonuclease 
ProteomicsA unique mRNA decapping complex in trypanosomes
Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2...
ORGANISM(S): Trypanosoma brucei 
2023-06-14 | PXD038550 | Pride
Xrna Mrna decay Apah-like phosphatase Alph1 Mrna decapping Trypanosoma Exoribonuclease 
ProteomicsMagoh, Y14, eIF4AIII interactomes by TurboID proximity labelling provide no evidence for a canonical EJC in (almost) intron-less trypanosomatids
In metazoans, mRNA quality is tightly monitored from transcription to translation. A key role lies with the exon junction complex (EJC) that is placed upstream of the exon-exon junction after splicing. The EJC inner core is composed of Magoh, Y14, eIF4AIII and BTZ and the outer core of proteins invo...
ORGANISM(S): Trypanosoma brucei 
2024-03-25 | PXD050946 | Pride
Mrna metabolism Mrna export control Exon junction complex Trypanosoma brucei 
ProteomicsProbing the trypanosome nuclear pore by TurboID proximity labelling – Part2
Previous BioID experiments targeting the nucleoporin (NUP) NUP158 (PMID: 36410438; PXD031245), as well as NUP110, NUP76 and NUP96 (PMID: 39206942; PXD047268) indicated that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labellin...
ORGANISM(S): Trypanosoma brucei 
2024-09-20 | PXD055934 | Pride
Bioid Trypanosoma brucei Porin Nuclear transport Nuclear pore 
ProteomicsProbing the trypanosome nuclear pore by TurboID proximity labelling
Previous BioID experiments targeting the nucleoporin (NUP) NUP158 and the nuclear transport factor MEX67 indicated (PMID: 36410438; PXD031245) that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labelling radius well below the s...
ORGANISM(S): Trypanosoma brucei 
2023-12-04 | PXD047268 | Pride
Bioid Trypanosoma brucei Porin Nuclear transport Nuclear pore 
ProteomicsCharacterization of a novel membrane complex required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila
In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon termed regulated exocytosis. A forward genetic approach uncovered MDL1 (Mucocyst Discharge with a ...
ORGANISM(S): Tetrahymena thermophila CU428 
2022-04-06 | PXD028372 | Pride
Exocytosis Mucocysts Ciliate Tetrahymena Lysosome Mdl1 
ProteomicsTrypanosoma brucei global proteome changes upon suramin exposure
To understand how suramin kills Trypanosoma brucei, we examined the impact on proteome at different timepoints
ORGANISM(S): Trypanosoma brucei 
2020-04-30 | PXD014215 | Pride
Trypanosoma brucei Drug mode of action Suramin 
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