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ProteomicsInteractome analysis of C. elegans synapses by TurboID-based proximity labeling
Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labeling protocol for C. elegans. We use this protocol to characterise the prote...
ORGANISM(S): Caenorhabditis elegans 
2021-09-15 | PXD027068 | Pride
Interactome caenorhabditis elegans turboid proximity proteomics biotin labeling 
ProteomicsTurboID-mediated proximity labeling of DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans
TurboID-mediated proximity labeling of the aging and stress regulatory transcription factor DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans. Endogenous DAF-16 was C-terminally tagged with the biotin ligase TurboID using CRISPR/Cas9- genome editing. In vivo proximity labeling of the ...
ORGANISM(S): Caenorhabditis elegans 
2025-12-29 | PXD053591 | Pride
Foxo Turboid Insulin / igf signaling Daf-16 Caenorhabditis elegans Proximity labeling 
ProteomicsAnalysis of C. elegans DAF-16 phosphorylation in different genetic backgrounds.
C. elegans DAF-16/FOXO phosphorylation in different genetic backgrounds was analyzed by mass spectrometry of affinity purified HA-tagged DAF-16. Genetic backgrounds studied include par-1 hypomorphic mutants, PAR-1 knockdown using an auxin-inducible degron (AID), daf-2(e1370) and akt-1(ok525) mutants...
ORGANISM(S): Caenorhabditis elegans 
2025-12-29 | PXD053590 | Pride
Foxo Par-1 Daf-16 Phosphorylation Daf-2 Mark Akt-1 
ProteomicsAn improved TurboID-based proximity labeling pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases
To review an improved proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes, the interactome of a presynaptic active zone protein, ELKS-1, was analyzed as proof of principle. A significant constraint on the sensitivity of TurboI...
ORGANISM(S): Caenorhabditis elegans 
2022-09-12 | PXD034639 | Pride
Synapse Imac Apex2 Proximity-dependent protein labeling Hrp Biotinylated carboxylase depletion Turboid Caenorhabditis elegans 
ProteomicsMALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity
The interaction partners of C. elegans soluble IL-17 signaling components were profiled using IP/MS. In vivo interactions were profiled for ACTL-1-FLAG, PIK-1-Myc, MALT-1::GFP, NFKI-1::GFP and their respective controls. Quantification of NFKI-1::GFP interactions was performed by labelling immunoprec...
ORGANISM(S): Caenorhabditis elegans 
2020-05-27 | PXD018000 | Pride
C. elegans Proteomics Neuroimmune communication Behaviour Signaling 
UnknownFinal results of DIADEM, a phase II study to investigate the efficacy and safety of durvalumab in advanced pretreated malignant pleural mesothelioma.
Not available
S-EPMC9808442 | biostudies-literature
UnknownEfficacy of n-3 polyunsaturated fatty acids and feasibility of optimizing preventive strategies in patients at high cardiovascular risk: rationale, design and baseline characteristics of the Rischio and Prevenzione study, a large randomised trial in general practice.
Not available
S-EPMC2909229 | biostudies-literature
UnknownCommon and rare variant association analyses in amyotrophic lateral sclerosis identify 15 risk loci with distinct genetic architectures and neuron-specific biology.
Not available
S-EPMC8648564 | biostudies-literature
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