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UnknownOptimisation of Downscaled Tandem Affinity Purifications to Identify Core Protein Complexes.
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S-EPMC3785125 | biostudies-literature
ProteomicsH4K20me2 SILAC Nucleosome Affinity Purification
Goal of this project was the identification of chromatin interacting proteins whose binding is differentially regulated by di-methylation of lysine 20 on histone H4 (H4K20me2). To achieve this unodified and H4K20me2-modified histone H4 were generated by native chemical ligation and assembled into re...
ORGANISM(S): Homo sapiens (Human) 
2019-02-27 | PXD009281 | Pride
H4k20me2 H4k20me0 Snap Silac Nucleosome affinity purification 
ProteomicsComprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits
Affinity purifications of CHD4 and CDK2AP2 to identify their interactors. The C-terminally tagged CHD4 has a C-terminal truncation to test whether this domain is required for the interaction with other NuRD subunits. GFP-purifications of both N- and C-terminally tagged CDK2AP2 were performed.
ORGANISM(S): Homo sapiens (Human) 
2020-12-07 | PXD017378 | Pride
Chd Cdk2ap2 Doc1r Nurd 
ProteomicsDissection of affinity captured LINE-1 macromolecular complexes
Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using dif...
ORGANISM(S): Homo sapiens (Human) 
2018-01-17 | PXD008542 | Pride
Line-1 Protein interactions Affinity proteomics L1 Mass spectrometry Biochemistry Retrotransposon Ribonucleoprotein Rnp Interactome 
ProteomicsMass Spectrometry of NOT gene-related purifications
Mass Spectrometry of NOT gene-related purifications, including NOT5 Tap Tag in WT and not4 deletion, the Ribosome Nascent Chain Complex, and aggregates in NOT5 Delta,
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-07-26 | PXD021580 | Pride
Aggregates Not5 Mass spectrometry Not4 Not genes Rnc Ribosome-nascent chain complex 
ProteomicsPull-down affinity purifications using di-nucleosomes decorated with enhancer-associated modifications and incorporating 200bp linker DNA
Characteristic features of chromatin states are not limited to particular epigenetic modifications but include other regulatory cues, such as linker DNA length, typically ranging from around 35-55 bp in most eu- and heterochromatin domains (Valouev et al, 2011; Voong et al., 2016, Cell) to over 200 ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-12 | PXD041443 | Pride
Ndrs H3k4me1 H3k27ac Pull-down 
ProteomicsComprehensive characterization of Minichromosome Maintenance Complex (MCM) protein interactions using affinity and proximity purifications coupled to mass spectrometry.
This project is the complete interactome of the Minichromosome Maintenance (MCM) complex by using AP-MS and BioID approaches for all the different MCM proteins. Overall, our analysis identified unique and shared interaction partners and proteins enriched for distinct biological processes including D...
ORGANISM(S): Homo sapiens (Human) 
2016-08-09 | PXD004089 | Pride
Ap-ms Bioid Mcm complex 
TranscriptomicsControlling for gene expression changes in transcription factor protein networks.
The development of affinity purification technologies together with mass spectrometric analyses of the purified protein mixtures (AP-MS) has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactio...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-GEOD-57027 | biostudies-arrayexpress
TranscriptomicsSmall RNA loading licenses Argonaute for assembly into a transcriptional silencing complex
We analyzed the small RNAs bound to the fission yeast RITS and ARC complexes, and to Ago1 in wild-type and ARC mutant cells, to gain insight into the molecular role of ARC. We found that ARC contains longer heterochromatic siRNAs than RITS, and that ARC subunit Arb1 is required for loading small RNA...
ORGANISM(S): Schizosaccharomyces pombe 
2015-03-01 | E-GEOD-65223 | biostudies-arrayexpress
ProteomicsSystematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa
Addressing the functionality of predicted genes remains an enormous challenge in the post-genomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2014-09-09 | PXD001199 | Pride
Bacteriophages P. aeruginosa Functional annotation Affinity purifications Interactomics 
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