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ProteomicsOxidized thioredoxin-1 restrains the NLRP1 inflammasome
To find cellular protein binding partners of the NLRP1 inflammasome, FLAG-tagged full length NLRP1 (or a GFP-FLAG protein control) was ectopically expressed in HEK 293T cells and affinity purified from cell lysates (Note: the NLRP1-FLAG-containing lysate samples were affinity purified in the presenc...
ORGANISM(S): Homo sapiens (Human) 
2022-11-04 | PXD037391 | Pride
Innate immunity Programmed cell death Inflammasome Chemical biology Pattern recognition receptors 
ProteomicsA chemical strategy for protease substrate profiling
Here, we introduce a simple method, termed CHOPS, for the discovery of protease substrates. CHOPS exploits a 2-pyridinecarboxaldehyde (2PCA)-biotin probe, which selectively biotinylates protein N-termini except those with proline in the second position. CHOPS can, in theory, discover substrates for...
ORGANISM(S): Homo sapiens (Human) 
2019-04-23 | PXD013019 | Pride
Chops Silac N-terminomics 
ProteomicsThe interaction between NLRP1 and oxidized TRX1 involves a transient disulfide bond: LC-MS/MS Dataset
LC-MS/MS analysis of a gel slice containing a disulfide-cross-linked peptide chains between thioredoxin-1 and the NACHT-LRR domains of NLRP1
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD047547 | Pride
Disulfide Lc-ms Nlrp1 Trx1 
ProteomicsGlobal proteomics of HEK293T cells treated with Val-boroPro for 48h
Global proteomics of HEK293T cells treated with Val-boroPro for 48h
ORGANISM(S): Homo sapiens (Human) 
2020-10-14 | PXD015978 | Pride
Val-boropro Tmtsixplex 
ProteomicsDPP9 activity and not protein binding inhibits the CARD8 inflammasome
Inflammasomes are multiprotein complexes formed in response to pathogens. NLRP1 and CARD8 are related proteins that form inflammasomes, but the pathogen-associated signal(s) and the molecular mechanisms controlling their activation have not been established. Inhibitors of the serine dipeptidyl pept...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD015047 | Pride
Val-boropro Card8 Dpp9 
UnknownA small molecule inhibitor prevents gut bacterial genotoxin production.
Not available
S-EPMC9889270 | biostudies-literature
ProteomicsTarget identification of CQ31, a selective activator of the CARD8 inflammasome
Target identification of CQ31 using the photoreactive diazirine-alkyne probe, CQ73.
ORGANISM(S): Homo sapiens (Human) 
2021-11-17 | PXD027707 | Pride
Pepd Xpnpep1 Cq31 Inflammasome Tmt-sixplex 
ProteomicsTarget identification of methyl bestatin
Target identification of methyl bestatin (MeBs) using the photoreactive diazirine-alkyne probe, CQ83.
ORGANISM(S): Homo sapiens (Human) 
2022-11-22 | PXD038233 | Pride
Aminopeptidase Methyl bestatin Bestatin 
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