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TranscriptomicsTumor Cell Survival Dependence on the DExH-Box Helicase DHX9
The ATP-dependent DExH/D-box helicase DHX9 is a key participant in a number of gene regulatory steps, including transcriptional, translational, microRNA-mediated control, DNA replication, and maintenance of genomic stability. DHX9 has also been implicated in maintenance of the tumorigenic process an...
ORGANISM(S): Mus musculus 
2016-05-19 | E-GEOD-76765 | biostudies-arrayexpress
TranscriptomicsLack of the pif1 helicase enhances zinc tolerance in S. cerevisiae
The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1D strain is tolerant to zinc overload. We demonstrate here that this effect is independent of the Pif1 helicase activity...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-31 | E-GEOD-18411 | biostudies-arrayexpress
ProteomicsMechanisms of MCM2-7 helicase activation and initial DNA melting at near base-pair resolution
During eukaryotic DNA replication initiation, inactive MCM2-7 double-hexamers assembled at replication origins must be converted into two active CMG helicases, yet how this transition is coupled to origin DNA unwinding in vivo remains unclear. Here, we identify a DNA-bound intermediate with an exten...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-06-26 | PXD080178 | Pride
Ssdna Helicase Unwinding Origins Dna replication Yeast Chip-exo Mcm2-7 Rpa S-phase 
ProteomicsDNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans
Assembly of the DNA helicase known as CMG (CDC45-MCM-GINS) is the key regulated step during DNA replication initiation in eukaryotes. Using the Caenorhabditis elegans embryo as a model system, we identify a new CMG assembly factor called DNSN-1, which associates with the BRCT-domain protein MUS-101...
ORGANISM(S): Caenorhabditis elegans 
2023-10-24 | PXD044240 | Pride
Donson C. elegans Meier gorlin syndrome Mus-101 Dnsn-1 Cmg helicase Dna replication 
GenomicsPost-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA
Eukaryotic genomes are replicated from many origin sites that are licensed by the loading of inactive double hexamers of the replicative DNA helicase, Mcm2-7. How eukaryotic origin positions are specified remains elusive. Here we show that, contrary to the bacterial paradigm, eukaryotic origins are ...
ORGANISM(S): Saccharomyces cerevisiae 
2016-01-07 | E-GEOD-69065 | biostudies-arrayexpress
ProteomicsRole of BLM helicase in augmenting the phosphorylation of DNA damage response (DDR) proteins.
Assessing the importance of BLM helicase upon DNA damage in the phosphorylation enhancement of DNA damage response (DDR) proteins via phosphoproteomic profiling in the presence and absence of BLM.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD048117 | Pride
Dna repair Atm Dna damage response Blm helicase 
ProteomicsER-associated degradation ligase HRD1 links ER stress to DNA damage repair by modulating the activity of DNA-PKcs
ER-associated degradation ligase HRD1 links ER stress to DNA damage repair by modulating the activity of DNA-PKcs
ORGANISM(S): Homo Sapiens 
PXD055020 |
GenomicsMap of DnaC localization in the presence or absence of PcrA (PcrA depletion)
The replicative helicase, DnaC, was immunoprecipitated in cell extract prior to, or following conditional depletion of PcrA The differential DnaC association at each genomic location was determined before/after PcrA depletion
ORGANISM(S): Bacillus subtilis subsp. subtilis str. JH642 
2015-12-13 | E-GEOD-75953 | biostudies-arrayexpress
ProteomicsStructure of the helicase core of Werner Helicase, a key target in microsatellite instability cancers
Loss of WRN, a DNA repair helicase, was identified as a strong vulnerability of microsatellite instable(MSI) cancers, making WRN a promising drug target. We show that ATP binding and hydrolysis are required for genome integrity and viability of MSI cancer cells. We report a 2.2 Å crystal structure o...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD018910 | Pride
Helicase Hdx Deuterium exchange Dna binding 
ProteomicsMCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage
Homologous recombination (HR) mediates the error-free repair of DNA double-strand breaks to maintain genomic stability. Here we characterize C17orf53/MCM8IP, an OB-fold containing protein that binds ssDNA, as a DNA repair factor involved in HR. MCM8IP-deficient cells exhibit HR defects, especially i...
ORGANISM(S): Homo sapiens (Human) 
2020-06-15 | PXD019156 | Pride
C17orf53 Mcm9 Mcm8 Mcm8ip Cisplatin Rpa Homologous recombination 
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