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ProteomicsProteome integral solubility alteration high-throughput proteomics assay identifies Collectin-12 as a non-apoptotic microglial caspase-3 substrate.
Caspases are a family of proteins mostly known for their role in the activation of the apoptotic pathway leading to cell death. In the last decade, caspases have been found to fulfil other tasks regulating the cell phenotype independently to cell death. Microglia are the immune cells of the brain re...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD032835 | Pride
Caspase-3 Solubility Pisa Non-apoptotic Sustrates Colec12 Proteomics Microglia 
ProteomicsHepatocyte thorns, a novel drug-induced stress response in human and mouse liver spheroids
The in vivo-relevant phenotype of 3D liver spheroids allows for long-term studies of e.g. novel mechanisms of chronic drug-induced liver toxicity. Using this system, we present a novel drug-induced stress response in human and murine hepatocyte spheroids wherein long slender filaments form after chr...
ORGANISM(S): Homo sapiens (Human) 
2022-05-26 | PXD033550 | Pride
Hepatocytes  spheroids  thorns  hepatotoxicity  keratins  3d culture 
ProteomicsA multi-target molecule restores immune response in lung cancer through intratumoral CD4+ cytotoxic lymphocytes recruitment
Background: Despite current therapeutic treatments including surgery, chemotherapy, radiotherapy and recently immunotherapy, the mortality rate of lung cancer stays high. Regarding lung cancer, epigenetic modifications altering cell cycle, angiogenesis and programmed cancer cell death are therapeut...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-16 | PXD042991 | Pride
Moa Expression proteomics Mass spectrometry Cd4 ctl Drug targets Mhc-ii Pisa assay Lung cancer Hdac6 
ProteomicsLineage-specific oncogenes drive growth of major forms of human cancer using common downstream mechanisms
Aim of the experiment was to identify changes in protein-protein interactions between proliferating and non-proliferating cells. For this purpose, RKO cells were treated with 20nM Trametinib, HCT116 with 8nM Trametinib, and NCI-H1975 100nM Osimertinib. At these concentrations, the wild type cell lin...
ORGANISM(S): Homo sapiens (Human) 
2025-07-17 | PXD037155 | Pride
Proteomics Pisa assay Protein solubility Lung cancer Proliferation Lc-ms/ms Colorectal cancer 
ProteomicsPISA Assay of B1 antibiotic on Neisseria gonorrhoeae
Study on antibiotic B1 and for target identification and mode of action characterization on Neisseria gonorrhoeae Strain, using Proteome Integral Solubility Alteration (PISA) Assay
ORGANISM(S): Neisseria gonorrhoeae 
2026-01-19 | PXD063107 | Pride
Proteomics Lc-ms Mode of action Antibiotic Target id Bacteria Pisa assay 
ProteomicsQuantitative proteomics of HT-1080 cells exposed to single-atom engineered graphene derivatives versus copper ionophore elesclomol and glutathione peroxidase 4 inhibitor RSL3
This dataset presents a comparative proteomic analysis of the human fibrosarcoma cell line HT-1080 in response to treatment with nitrogen-doped graphene acid (NGA) and the single atom engineered derivatives, NGA-Fe3+ and NGA-Cu2+. To further investigate the role of metal-induced cell death pathways,...
ORGANISM(S): Homo sapiens (Human) 
2026-02-02 | PXD067483 | Pride
Graphene Expression proteomics Ferroptosis Single atom engineering Cuproptosis Elesclomol Fibrosarcoma 
ProteomicsSorCS2 controls Wnt signaling homeostasis during neurodevelopment by regulating Ror2 activity and trafficking
Wnt signaling orchestrates key developmental processes, yet mechanisms restricting its spatial and temporal activity remain incompletely understood. Here we identify SorCS2, a VPS10p-domain sorting receptor, as a conserved regulator of Wnt signaling homeostasis. Integrating proteomics, cell biology,...
ORGANISM(S): Mus musculus (Mouse) 
2026-10-02 | PXD085067 | Pride
Wnt/β-catenin signaling Wnt5a Dopaminergic circuits Wnt homeostasis Ror2 Intracellular sorting Sorcs2 Tcf4 Wnt1 Noncanonical wnt signaling 
ProteomicsDeep learning-enabled discovery of antibiotics effective against Neisseria gonorrhoeae
Neisseria gonorrhoeae is a Gram-negative, sexually transmitted pathogen that poses a major public health threat due to rapidly increasing resistance to all recommended antibiotics. Addressing this crisis requires more efficient approaches to antibiotic discovery and the replenishment of the dwindlin...
ORGANISM(S): Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) 
2026-05-07 | PXD067696 | Pride
Deep learning Antibiotics Drug discovery Infectious disease Neisseria gonorrhoeae Pisa assay Proteomics 
ProteomicsHigH-ratiO partiaL proteolysiS with carriER proteome (HOLSER) Enables Global Structure Profiling and Site-resolved Elucidation of Ligand-Protein Interactions
Understanding how cellular proteins interact with their environment, including endogenous and exogeneous molecules, is critical for elucidating mechanisms of cellular regulation and drug action. Partial proteolysis-based techniques offer peptide-level resolution of ligand-induced conformational chan...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD069119 | Pride
Proteomics Tmt Drug traget deconvolution Lc-ms Machanism of action Partial proteolysis 
ProteomicsProteome Integral Solubility Alteration (PISA): a high-throughput proteomics assay for target deconvolution
Various agents, including drugs as well as non-molecular influences, induce alterations in the physic-chemical properties of proteins in cell lysates, living cells and organisms. These alterations can be probed by applying a stability-modifying factor, such as elevated temperature, to a varying degr...
ORGANISM(S): Homo sapiens (Human) 
2020-04-01 | PXD013182 | Pride
Protein stability Target deconvolution Tmt Thermal profiling Quantitative proteomics 
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