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ProteomicsPlasmodium falciparum bromo-domain containing epigenetic protein complexes recruited to modified histone tails as identified by histone peptide pulldown (HPP)-MS/MS and further characterized by GFP-IP-MS/MS and HA-IP-MS/MS
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
Epigenetic complex Bromo-domain protein Hpp Gcn5 Gfp-ip Histone peptide pulldown Interactor Plasmodium falciparum Taf1 Histone acetylation Ha-ip Reader complex Histone methylation 
ProteomicsHistone H3 tail interactome in MPC11 murine myeloma cells
We have used peptide capture in conjunction with super-SILAC quantification to carry out an unbiased high-throughput analysis of the composition of protein complexes that bind to histone H3K9/S10 and H3K27/S28 methyl-phospho modifications. We compared binding profiles of nuclear proteins from MPC11 ...
ORGANISM(S): Mus musculus (Mouse) 
2016-05-04 | PXD001273 | Pride
Lc-msms Histone modification Peptide capture Histone h3 tail interactome 
ProteomicsTGM2-mediated histone serotonylation promotes HCC progression via MYC signalling pathway
TGM2 IP-MS was performed in MHCC-97H cells to screen the interactors of TGM2. 5-PT pulldown-MS was perfromed in MHCC-97H cells to identify proteins modified by serotonylation.
ORGANISM(S): Homo Sapiens 
2024-12-06 | PXD058674 |
ProteomicsLFQ-MS analysis of nucleosome pulldown experiments with symmetric and asymmetric nucleosomes
Recombinant mononucleosomes were generated carrying H3K4me3 and H3K27me3 in symmetric or asymmetric monovalent or asymmetric bivalent conformation, immobilized on beads and subjected to pulldown assays with mouse ESC nuclear extract. Binding was analyzed by quantitative LFQ-MS.
ORGANISM(S): Mus musculus (Mouse) 
2025-01-02 | PXD015990 | Pride
Chromatin Transcription Histone acetylation Embryonic stem cells Differentiation Bivalent domains Histone methylation 
ProteomicsThe functional interactome of site-specifically ubiquitylated linker histone H1
Decoding the role of histone posttranslational modifications (PTMs) is key to understand the fundamental process of epigenetic regulation. While this process is well studied for core histones and many of their PTMs, this is not the case for linker histone H1 in general and its ubiquitylation in part...
ORGANISM(S): Homo sapiens (Human) 
2021-07-20 | PXD020686 | Pride
Epigenetics Linker histone h1 Ubiquitin Posttranslational modification (ptm) Histone code Chromatin 
ProteomicsSite-specific ubiquitylation acts as a regulator of linker histone H1
Decoding the role of histone posttranslational modifications (PTMs) is key to understand the fundamental process of epigenetic regulation. While this process is well studied for core histones and many of their PTMs, this is not the case for linker histone H1 in general and its ubiquitylation in part...
ORGANISM(S): Homo sapiens (Human) 
2021-06-07 | PXD025258 | Pride
Epigenetics Linker histone h1 Ubiquitin Posttranslational modification (ptm) Histone code Chromatin 
ProteomicsLysine fatty-acyl H3 peptide pulldown with HeLa SILAC nuclear extract
Histone H3 peptides with acyl modification on either K9 or K27 were used for peptide pull-down with stable isotope (light: K0R0, heavy: K8R10) labeled Hela nuclear extract. The acyl modifications are: acetyl (Ac), propionyl (Pro), Butanoyl (But), crotonyl (Cr), 3-hydroxy butanoyl (bHB), succinyl (Su...
ORGANISM(S): Homo sapiens (Human) 
2019-10-02 | PXD015233 | Pride
Orbitrap elite Homo sapiens 
ProteomicsAza-SAHA Derivatives are Selective Histone Deacetylase 10 Chemical Probes That Inhibit Polyamine Deacetylation
We report the first selective chemical probes for histone deacetylase 10 (HDAC10) with unprecedented selectivity over other HDAC isozymes. HDAC10 deacetylates polyamines and has a distinct substrate specificity, making it unique among the 11 zinc-dependent HDAC hydrolases. Taking inspiration from HD...
ORGANISM(S): Homo sapiens (Human) 
2026-06-02 | PXD032671 | Pride
Histone deacetylase Polyamines Be(2)-c Drug discovery Chemical proteomics Chemical biology Chemical probes Lc-ms/ms 
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
UnknownThe Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation.
Not available
S-EPMC2924014 | biostudies-literature
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