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ProteomicsPersonalised phosphoproteomics extracts functional signalling
Protein phosphorylation dynamically integrates environmental and cellular information to control virtually all biological processes. Identifying functional phosphorylation amongst the thousands of phosphosites regulated by a perturbation at a global scale is a major challenge. Here, we introduce pe...
ORGANISM(S): Homo sapiens (Human) 
2021-12-06 | PXD027198 | Pride
Phosphoproteomics Human Exercise Skeletal muscle Insulin 
ProteomicsTSC-Insensitive Rheb Mutations Induce Oncogenic Transformation Through a Combination of Hyperactive mTORC1 Signalling and Metabolic Reprogramming
The mechanistic target of rapamycin complex 1 (mTORC1) is an important regulator of cellular metabolism that is commonly hyperactivated in cancer. Recent cancer genome screens have identified multiple mutations in Ras-homolog enriched in brain (Rheb), the primary activator of mTORC1, that might act ...
ORGANISM(S): Mus musculus (Mouse) 
2021-04-22 | PXD017006 | Pride
Proteomes Rheb Lc-msms Mtor 
ProteomicsThe genetic and dietary landscape of the muscle insulin signalling network
Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in the skeletal muscle of five genetically distinct inbred mouse strains exposed to two controlled dietary environments, with and without acute insulin treatment. Almost half of the insulin-regulated phosphoproteome w...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-21 | PXD041229 | Pride
Phosphoproteomics Glucose regulation Phosphoproteome Diet Environment Genetic Skeletal muscle Mouse 
ProteomicsSex-specific Phosphoproteome Responses to Calorie Restriction and Insulin in Skeletal Muscle from Older Rats
Aging impairs skeletal muscle insulin sensitivity, yet the underlying molecular mechanisms remain incompletely understood. Here, we examined how short-term caloric restriction (CR) alters muscle insulin action and signaling in aged rats (22 months old) of both sexes. After 8 weeks of CR or ad libitu...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-11-24 | PXD064514 | Pride
Calorie restriction Phosphoproteome Rats Skeletal muscle Muscle 
ProteomicsDeep 3T3-L1 adipocyte proteome at 10 days post-differentiation
Here we conducted a comprehensive analysis of the proteome of the widely used 3T3-L1 murine adipocyte laboratory cell line at 10-days post-differentiation.
ORGANISM(S): Mus musculus (Mouse) 
2023-02-13 | PXD039532 | Pride
Mouse Adipocytes 
ProteomicsAdipose tissue protein kinase D (PKD): regulation of signalling networks and its effect on metabolism
The protein kinase D (PKD) family of three highly homologous isoforms (PKD1, PKD2, and PKD3) are implicated as nutrient sensing signalling kinases that regulate the response of adipose and other tissues to the nutrient environment. However, the physiological role of adipose tissue PKD and the cellul...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-27 | PXD056412 | Pride
Human Adipose tissue Phosphoproteomics 
ProteomicsAlpha Protein Kinase 3 Gene Therapy Restores Heart Function in Mouse and Human Models of Cardiomyopathy
Truncating variants in Alpha Kinase 3 (ALPK3) cause severe cardiomyopathy for which there is no curative treatment. Here, we established an AAV-mediated gene replacement therapy to deliver full-length human ALPK3. This approach prevented cardiomyopathy development in neonatal Alpk3 mutant mice and r...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-25 | PXD077620 | Pride
Phosphoproteomics Proteomics Cardiomyopathy 
ProteomicsIGFBP2 Mediates Human iPSC-Cardiomyocyte Proliferation in a Cellular Contact-Dependent Manner
Inducing cardiomyocyte proliferation in situ presents a promising approach for cardiac regeneration following myocardial injury; however, mature cardiomyocytes exhibit inhibitory mechanisms that suppress proliferative signaling. This study investigates the role of cell-cell contact as a key regulato...
ORGANISM(S): Homo sapiens (Human) 
2025-12-15 | PXD061405 | Pride
Cardiac regeneration Cardiac Ipsc Ipscs Cardiomyocyte Cell proliferation Human Igfbp2 
ProteomicsPhosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
Here we employed phosphoproteomics to delineate insulin signal transduction in adipocyte cells and adipose tissue. Across a range of insults triggering insulin resistance, we observed marked rewiring of the insulin signaling network. This included both attenuated insulin-responsive phosphorylation, ...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-17 | PXD032913 | Pride
Post-translational modification Adipocyte Mass spectrometry Fat Phosphoproteomics Gsk3 Tissue Proteomics Adipose Signal transduction Phosphorylation Insulin signalling Insulin resistance 
ProteomicsProteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet
Pancreatic islets are essential for maintaining physiological blood glucose levels, and declining islet function is a hallmark of type 2 diabetes. Here we employ mass spectrometry (MS)-based proteomics to systematically analyse purified islets from 9 genetic or diet-induced mouse models representing...
ORGANISM(S): Mus musculus (Mouse) 
2021-10-08 | PXD024522 | Pride
Pancreas Alpha cells Beta cells Islets Library Proteome 
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