Sort   by:  
 Page size 
GenomicsMulti-omics analysis using antibody-based in situ biotinylation technique reveals the mechanism of Cajal body formation
Multi-omics analysis using antibody-based in situ biotinylation technique reveals the mechanism of Cajal body formation
PRJNA987706 | ENA
ProteomicsIn Situ Proximity Labeling Identifies Lewy Pathology Molecular Interactions in the Human Brain
The intracellular misfolding and accumulation of alpha-synuclein into structures collectively called LP is a central phenomenon for the pathogenesis of synucleinopathies including Parkinson’s disease (PD) and Dementia with Lewy Bodies (DLB). Understanding the molecular architecture of LP is crucial ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD028830 | Pride
In situ Biotinylation by antigen recognition Neuropathology Lewy pathology Alpha-synuclein 
ProteomicsProfiling the interactome of oligonucleotide drugs by proximity biotinylation
Drug-ID applies proximity biotinylation to identify drug-protein interactions inside living cells. The covalent conjugation of a drug molecule with a biotin ligase enables targeted biotinylation of the drug-bound proteome and its identification by SILAC-MS/MS. We investigate the interactome of two w...
ORGANISM(S): Homo sapiens (Human) 
2024-01-09 | PXD045992 | Pride
Antisense oligonucleotide Basu Proximity biotinylation Drug protein interactome Live cell Biotin ligase Hdac inhibitors Silac In situ biotinylation Bet inhibitors Bioid 
ProteomicsIdentification of the Tau aggresome in progressive supranuclear palsy by in situ biotinylation by antibody recognition
Here, we used an unbiased proteomic approach, which combines antibody-mediated proximity-detection of co-aggregated proteins together with mass spectrometry. Biotinylation by antibody recognition (BAR) is a recently developed method, by which a primary antibody recognises the target of interest in f...
ORGANISM(S): Homo sapiens (Human) 
2023-02-27 | PXD028237 | Pride
Tauopathy  aggresome  biotinylation 
ProteomicsDefinition of the fibroblast adhesome using multiplexed proximity biotinylation
Integrin adhesion complexes (IACs) bridge the extracellular matrix to the actin cytoskeleton and transduce signals in response to both chemical and mechanical cues. The composition, interactions, stoichiometry and topological organisation of proteins within IACs are not fully understood. To address ...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-01 | PXD017241 | Pride
Fibroblast Adhesome Bioid 
TranscriptomicsMulti-omics analysis using antibody-based in situ biotinylation technique reveals the mechanism of Cajal body formation
Membrane-less subcellular compartments such as cytoplasmic bodies, nuclear bodies, and paraspeckles play important roles in a variety of cellular functions. Although many of the techniques for identifying the components of cellular bodies were developed to address their function, no technique to com...
ORGANISM(S): Homo sapiens 
2024-10-01 | GSE235853 | GEO
ProteomicsSuper-resolution proximity labeling method with enhanced direct identification of biotinylation sites
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein-protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotin...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047979 | Pride
Biotinylation site Apex2 Turboid Lc-ms Membrane protein topology Bioid Proximity labeling 
GenomicsEstablishing the Oocyte Proteome at the Primordial Follicle Stage and Cisplatin-induced Shifts through in situ APEX2-driven Biotinylation
Establishing the Oocyte Proteome at the Primordial Follicle Stage and Cisplatin-induced Shifts through in situ APEX2-driven Biotinylation
PRJNA1045694 | ENA
ProteomicsiCAB: Development of an in situ cell-type specific proteome analysis method using antibody-mediated biotinylation
Since proteins are essential molecules exerting cellular functions, decoding proteome changes is the key to understanding the normal physiology and pathogenesis mechanism of various diseases. However, conventional proteomic studies are often conducted on tissue lumps, in which multiple cell types ar...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-12 | PXD046362 | Pride
Neuron Astrocytes Microglia. Brain Mass spectrometry Cell-type-specific proteome analysis Immunohistochemistry Biotin-tyramide 
UnknownDevelopment of an in situ cell-type specific proteome analysis method using antibody-mediated biotinylation.
Not available
S-EPMC10312661 | biostudies-literature
Sort   by:  
 Page size