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ProteomicsGlobal Enrichment of Low-Abundance Peptide Hormones in Plasma for Discovery and Quantitation
We describe a method for global enrichment of active peptide hormones from blood plasma for both new hormone discovery and quantitative analysis. To extend the dynamic range of plasma proteomics, we employed high-resolution size exclusion chromatography to purify the peptide hormones and a powerful ...
ORGANISM(S): Homo sapiens (Human) 
2019-07-16 | PXD011797 | Pride
Human  plasma  intermittent fasting  peptide hormone  insulin  hepcidin  glp-1  igf-1. 
ProteomicsCab45-dependent facilitation of insulin secretory granule biogenesis and its dysregulation in type 2 diabetes
Germanos M1*. Yau B1*. Taper M1. An Y1. Yeoman C1. Wilson A1. Cattin-Ortolá J2. Maslar D3. Tong J1. Naghiloo S1. Needham E4. Diaz-Vegas A4. Larance M1. Thomas H5. Seidah NG6. von Blume J7. James DE4. Thorn P1. Ailion M2. Asensio C3. Kebede MA1. 1 School of Medical Sciences, Charles Perkins Centre, ...
ORGANISM(S): Rattus rattus (Black rat) 
2024-12-02 | PXD052042 | Pride
Islet Cab45 Insulin 
Proteomics3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips
Mass spectrometry (MS) is a fundamental technology for the study of proteomics. A core technique for MS analysis is solid-phase extraction (SPE) for the removal of contaminants and sample concentration, and StageTips are one of the most commonly used devices used for SPE in modern proteomics workflo...
ORGANISM(S): Homo sapiens (Human) 
2019-03-21 | PXD009324 | Pride
3d-printed Stagetip 96-well 
ProteomicsClpC1-targeting peptide natural products differentially dysregulate the proteome of Mycobacterium tuberculosis
Targeting the protein quality control system in Mycobacterium tuberculosis offers a powerful and largely untapped opportunity for antibiotic development. The ClpC1:ClpP1P2 protease is an essential component of the system and is involved in both regulatory and stress-related protein degradation. Seve...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2025-12-14 | PXD057335 | Pride
ProteomicsRibo Mega-SEC Provides a Rapid, Efficient and Reproducible Analysis of Mammalian Polysomes and Ribosomal Subunits
We describe Ribo Mega-SEC, a powerful approach for the separation and biochemical analysis of mammalian polysomes and ribosomal subunits using Size Exclusion Chromatography and uHPLC, which was achieved within 15 min from sample injection to fraction collection. Ribo Mega-SEC reproducibly shows tran...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-10 | PXD008913 | Pride
Ribosome 60s 40s Polysome Liver Sec 
ProteomicsMass spectrometry-based proteomic analysis of the liprin-α1 interactome in MIN6 cells
Accumulating evidence suggests that insulin secretion in pancreatic β cells is regulated by mechanisms analogous to synaptic vesicle release at the neuronal presynaptic active zone (AZ). Neurotransmitter release is tightly regulated in magnitude, space and time by a presynaptic macromolecular comple...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-09 | PXD049219 | Pride
Presynaptic complex Pancreatic β cell Min6 Active zone Insulin exocytosis 
ProteomicsThe activity of the Caenorhabditis elegans Aquarius helicase EMB-4 enables the nuclear RNAi machinery to engage nascent RNA
Germline small RNA pathways initiate silencing of repetitive elements in animals and an interplay of nuclear small RNAs and chromatin modifications maintain this silencing, protecting the germline from spreading of transposable elements. In C. elegans germline, nuclear argonaute protein HRDE-1 initi...
ORGANISM(S): Caenorhabditis elegans 
2017-06-30 | PXD004416 | Pride
Silac Nematode Emb-4 
Proteomics¬Macropinocytosis mediates metabolic resistance to loss of glutamine transport in triple-negative breast cancer
Although a nonessential amino acid in normal cells, the demand for glutamine is dramatically increased throughout malignant transformation, supporting a range of metabolic processes including mitochondrial ATP production, protein synthesis, purine and pyrimidine biosynthesis. We previously showed th...
ORGANISM(S): Homo sapiens (Human) 
2024-09-16 | PXD053894 | Pride
Hcc Cancer Asct2 
ProteomicsUltra-sensitive platelet proteome maps the O-glycosylation landscape and identifies a new form of domain-specific O-fucosylation
Platelet activation induces the secretion of proteins that promote platelet aggregation and inflammation. However, detailed analysis of the released platelet proteome is hampered by platelets’ tendency to pre-activate during their isolation and a lack of sensitive protocols for low abundance releasa...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD045535 | Pride
O-glycosylation Platelet 
ProteomicsSCAMP3 is a component of insulin granules in MIN6 -cells and regulates insulin secretion and content
Pancreatic -cells in the islets of Langerhans are key to maintaining glucose homeostasis, by secreting the peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), that have recently been considered to have intrinsic structures and proteins that regulate ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD051136 | Pride
Pancreas Beta cell Insulin Scamp3 Islet 
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