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ProteomicsQuantitative Myogenesis C2C12 Proteome with Dimethyl-Labelling
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. In order to monitor the quantiative changes in C2C12 myoblast during myogenesis, a quantitative dimethyl-labelling approach was performed with d0 myoblasts, d5 myotubes and electrical puls stimulated...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD004960 | Pride
Muscle differentiation Myoblasts Dimethyl C2c12 cells Myotubes 
ProteomicsC-terminal FLNc in vivo kinase assay
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. Since a role of the Z-disc for signal integration and transduction was recently suggested, its precise phosphorylation landscape warranted in-depth analysis. We therefore established a site-resolved ...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD004151 | Pride
Flnc pkc gö6976 
ProteomicsLarge-scale in vitro kinase assay with FLNc d23-24 and PKC alpha
Large-scale in vitro kinase assay with human and murine FLNc and its site mutants and PKC alpha.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-01-02 | PXD003185 | Pride
Lc-ms In vitro kinase assay Flnc Pkc alpha 
ProteomicsIn vitro calpainolysis assays - Myofibrillar Z-discs are a protein phosphorylation hot spot with PKC α modulating protein dynamics
In vitro calpainolysis in combination with top down mass spectrometry reveals the calpain-1 cleavage sites in human and murine carboxy terminal regions of FLNc
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2017-01-02 | PXD003216 | Pride
Calpain Top down ms Flnc 
ProteomicsC2C12 Phosphoproteome - Myofibrillar Z-discs are a protein phosphorylation hot spot with PKC α modulating protein dynamics
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. Since a role of the Z-disc for signal integration and transduction was recently suggested, its precise phosphorylation landscape warranted in-depth analysis. We therefore established a site-resolved ...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD003211 | Pride
Tio2 C2c12 myotubes Scx Phosphoproteomics 
ProteomicsIGF-1 stimulated C2 skeletal myotube phosphoproteome
Skeletal muscle is known to adapt dynamically to changes in workload by regulatory processes of the phosphatidylinositide 3-kinase (PI3K)/Akt pathway. We performed a global quantitative phosphoproteomics analysis of contracting mouse C2 myotubes treated with insulin growth factor 1 (IGF-1) as contro...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-25 | PXD016721 | Pride
Silac Phospho-proteomics C2 Myotubes 
ProteomicsFILIP1 CT pull-down
FILIP1 was identified as potential new filamin C interaction partner by a SILAC-based BioID experiment. Interaction of the two protein via the carboxy-terminal end of FILIP and domain 18-21 of filamin C was prooven by yeast-2-hybrid sreens and dot-blot assays, respectively. To verify this interactio...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-06 | PXD008875 | Pride
C2c12 myotubes Filip1 Filamin c 
ProteomicsFilamin C domain 18-21 in vitro kinase assay
In vitro kinase assay with human filamin C domain 18-21 recombinantly expressed in E.coli, purified by Ni2+-NTA beads and incubated with PKC alpha, Akt or a combination of both to identify kinase specific phosphorylation sites.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD008893 | Pride
Filamin c Akt In vitro kinase assay Pkb Pkcalpha 
ProteomicsFilaminC pull-down experiment from myotube lysates
This project was designed to identify a difference in the binding affinity of FILIP1 to different FLNc variants. To this end FLNc domain 1-3 and FLNc d18-21 were used as bait for a pull-down analysis.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-05-25 | PXD017670 | Pride
Pull-down Myotube lysate Flnc 
ProteomicsFLNc S2233 S2236 PRM analysis
Skeletal muscle is known to adapt dynamically to changes in workload by regulatory processes of the phosphatidylinositide 3-kinase (PI3K)/Akt pathway. We performed a global quantitative phosphoproteomics analysis of contracting mouse C2 myotubes treated with insulin growth factor 1 (IGF-1) or LY2940...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-30 | PXD009228 | Pride
Filamin c Akt Pkc Myocytes 
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