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ProteomicsPhospho-Proteome Analysis of ROCK1 Conditional Knock-Out (ROCK1cKO) Mouse B-Cells under various in vitro stimulation conditions by LC-MS/MS
To better delineate how ROCK1 controls the biochemical state of activated B cells, we next conducted a phospho-proteomic analysis. In vitro stimulated WT and CD23-Rock1 B cells were harvested, subjected to pS/pT TMT and total proteome TMT, and results analyzed by Proteome Discoverer.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD059659 | Pride
Stimulation conditions Mouse primary b-cells Rock1cko Lc-ms/ms 
ProteomicsThe oncogenic action of NRF2 depends on de-glycation by Fructosamine-3-kinase (FN3K)
The NRF2 transcription factor controls a cell stress program that is implicated in cancer and there is great interest in targeting NRF2 for therapy. We show that NRF2 activity depends on Fructosamine-3-kinase (FN3K) - a kinase that triggers protein de-glycation. In its absence, NRF2 is extensively g...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-07-08 | PXD014334 | Pride
Hepatocellular carcinoma (hcc) Age Glycation Fn3k Nrf2 
Proteomics2 integrins impose a mechanical checkpoint on macrophage phagocytosis
Phagocytosis is an intensely physical process that depends on the mechanical properties of both the phagocytic cell and its chosen target. Here, we employed differentially deformable hydrogel microparticles to examine the role of cargo rigidity in the regulation of phagocytosis by macrophages. Where...
ORGANISM(S): Mus musculus (Mouse) 
2024-08-22 | PXD049434 | Pride
Mouse Lc-ms 
ProteomicsCopper depletion modulates OXPHOS in SOX2/OCT4+ cancer cells to impair triple negative breast cancer metastasis.
Copper serves as a co-factor for a host of metalloenzymes that contribute to malignant progression. The orally bioavailable copper chelating agent tetrathiomolybdate (TM) has been associated with a significant survival benefit in high-risk triple negative breast cancer (TNBC) patients. Despite these...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-10-26 | PXD027089 | Pride
Metabolism Mitochondria Copper Oxphos Ampk Sox2/oct4+ cells Metastasis Triple negative breast cancer Tetrathiomolybdate 
ProteomicsTransfer of antigen target into CAR T cells via trogocytosis
To characterize transfer of molecules from target cells into CAR T cells via trogocytosis we cultured NALM-6 leukemia cell line expressing a CD19-mCherry fusion protein with CAR T cells. NALM6-CD19-mCherry were loaded with heavy amino acid and cocultured with CAR T cells for 1 hour. CAR T cells wer...
ORGANISM(S): Homo sapiens (Human) 
2019-03-29 | PXD012833 | Pride
T cell Car t cell Leukemia Trogocytosis Cd19 
ProteomicsChromosomal instability adaptation dependencies confer unique targetable vulnerabilities in therapy refractory lethal prostate cancer
Chromosomal instability (CIN), an ongoing rate of chromosome missegregation during mitosis, is a defining feature of cancer. However, high chromosomal aberrations are detrimental for cell fitness. Here we investigated mechanisms allowing lethal prostate cancer (PCa) to tolerate and survive increasin...
ORGANISM(S): Homo sapiens (Human) 
2023-01-14 | PXD028271 | Pride
Mastl expression Chromosomal instability 
ProteomicsMechanical confinement governs phenotypic plasticity in melanoma
Phenotype switching is a form of cellular plasticity in which cancer cells reversibly move between two opposite extremes - proliferative versus invasive states. While it has long been hypothesised that such switching is triggered by external cues, the identity of these cues has remained elusive. Her...
ORGANISM(S): Homo sapiens (Human) 
2025-08-26 | PXD060265 | Pride
Lc-ms Hmgb2 Melanoma 
ProteomicsBridge-like lipid transfer protein 3A (BLTP3A) is associated with membranes of the late endocytic pathway and is an effector of CASM
Recent studies have identified a family of rod-shaped proteins which includes VPS13 and ATG2 and are thought to mediate unidirectional lipid transport at intracellular membrane contacts by a bridge-like mechanism. Here, we show that one such protein, BLTP3A/UHRF1BP1, associates with VAMP7-positive v...
ORGANISM(S): Homo sapiens (Human) 
2025-08-28 | PXD056338 | Pride
Lysosome Lysosomal membrane damage Bltp Bltp3b Bltp3a Casm Rab7 Bridge-like lipid transfer protein 
ProteomicsTelmisartan is neuroprotective in a hiPSC-derived spinal microtissue model for C9orf72-ALS via inhibition of neuroinflammation
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease characterized by motor neurons (MN) loss. Mutations in C9orf72 are the leading genetic cause of ALS and involve hexanucleotide repeat expansions (HRE) in the noncoding region of the gene. Neuroinflammation is a key featur...
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD057067 | Pride
Neuroinflammation  neurodegeneration  amyotrophic lateral sclerosis  c9orf72  human pluripotent stem cells  cellular interactions  motor neurons  astrocytes  microglia  spinal cord  triculture  three-dimension  microtissue  drug screen  sartans  telmisartan 
ProteomicsGPATCH8 modulates mutant SF3B1 mis-splicing and pathogenicity in hematologic malignancies
Mutations in the RNA splicing factor gene SF3B1 are common across hematologic and solid cancers and result in widespread alterations in splicing, but therapeutic means to correct this mis-splicing do not exist. Here, we utilize synthetic introns uniquely responsive to mutant SF3B1 to identify trans ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD044973 | Pride
Sugp1. Sf3b1 Splicing Dhx15 Gpatch8 Leukemia Myelodysplastic syndromes Rna G-patch domain 
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