Sort   by:  
 Page size 
ProteomicsLC-MS and gel-based comparison of DPSCs and PDLSCs
Tooth pulp contains various types of cells such as endothelial cells, neurons, fibroblasts, osteoblasts, osteoclasts, and odontoblasts. As well as cells that are called "postnatal dental pulp stem cells" (DPSCs). Also, four more types of dental MSC-like populations were identified and characterized:...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD027719 | Pride
Osteogenic differentiation Mscs Timstof pro Pdlscs 2d dige Dpscs Ion mobility 
ProteomicsComparative analysis of DDA- and DIA-PASEF methods for proteomics analysis of human valve interstitial cells and osteoblasts during osteogenic differentiation
Shotgun proteomics is one of the key "omics" methods, the methodology of which is rapidly developing. Mass spectrometers of the TimsToF series (Bruker) with ion mobility are one of the young technological platforms for shotgun proteomics in which both data dependent (DDA) and data independent acquis...
ORGANISM(S): Homo sapiens (Human) 
2024-11-12 | PXD036566 | Pride
Data independent acquisition Pasef Shotgun proteomics Homo sapiens Timstof pro Calcific aortic valve disease 
ProteomicsProteolytic degradation is the culprit behind a bioprosthetic heart valve failure
Whereas the risk factors for structural valve deterioration (SVD) of glutaraldehyde (GA)- treated bioprosthetic heart valves (BHVs) are well-studied, those responsible for the failure of next-generation BHVs fixed with alternative chemicals remain largely unknown. Here, we collected 11 ethylene glyc...
ORGANISM(S): Homo sapiens (Human) 
2022-12-25 | PXD035113 | Pride
Human Pasef Timstof pro Calcific aortic valve disease Bioprosthetic heart valve 
ProteomicsCalciprotein particles cause physiologically significant pro- inflammatory response in endothelial cells and systemic circulation
Calciprotein particles (CPPs) represent self-assembling scavengers of excessive Ca2+ and PO43- ions which are formed in the human serum under the guidance of acidic proteins termed mineral chaperones (e.g., fetuin-A and albumin). While protecting the human organism from extraskeletal calcification, ...
ORGANISM(S): Homo sapiens (Human) 
2022-11-30 | PXD038017 | Pride
Human Shotgun proteomics Timstof pro Calciprotein particles Calcium Endothelium 
ProteomicsImpact of Folded-State Protein Filaments on the Yeast Proteome
The formation of large polymeric structures without protein unfolding and aggregation, such as cytoskeletal and enzyme filaments, is crucial for normal cellular function. We term this type of assembly agglomeration in contrast to protein aggregation caused by protein misfolding. Several agglomeratio...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-08-21 | PXD052597 | Pride
Aggregation Agglomeration Yeasts Filaments Proteostasis 
ProteomicsProfiling the Interactome of Folded-State Protein Filaments in Yeast
The formation of large polymeric structures without protein unfolding and aggregation, such as cytoskeletal and enzyme filaments, is crucial for normal cellular function. We term this type of assembly agglomerates. There are several agglomeration-associated diseases, such as sickle cell disease, cau...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-08-21 | PXD052474 | Pride
Aggregation Agglomeration Yeasts Proteostasis 
ProteomicsProteomic profiling of carotid atherosclerotic plaques and adjacent intact arterial segments
Although it is well known that the extracellular matrix composition significantly impacts the development of neointimal hyperplasia and atherosclerosis, the biochemical cues regulating the cell-ECM interactions within the neointima remain obscure. Here, we pairwise collected 14 atherosclerotic plaqu...
ORGANISM(S): Homo sapiens (Human) 
2025-06-05 | PXD035173 | Pride
Atherosclerosis Timstof pro Diapasef Data-independent acquisition 
ProteomicsProteomics analysis of physiological effect of ZBTB16 (PLZF) on osteogenic differentiation of human valve interstitial cells (VICs) by DDA PASEF on TimsToF Pro
During fundamental proteotranscriptomic analysis of human valve interstitial cells (VICs) osteogenic differentiation, we revealed ZBTB16 (PLZF) as one of the most upregulated proteins. Thus, to investigate its physiological role in VICs osteogenic differentiation we performed its overexpression by l...
ORGANISM(S): Homo sapiens (Human) 
2022-10-21 | PXD031572 | Pride
Zbtb16  plzf  osteogenic differentiation  valve interstitial cells  pasef  timstof pro 
ProteomicsMolecular mechanisms of endothelial osteoinductive/osteosupressive properties
In vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in oste...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD037385 | Pride
Osteoblasts Timstof pro Osteogenic differeniation Endothelium Huvec 
ProteomicsProteomics comparison of human valve interstitial cells from healthy patients and donors with CAVD
Calcific aortic valve disease (CAVD) is an slowly progressive calcification of heart valve which leads to aortic stenosis. The only existing treatment of CAVD is surgical replacment of calcified valve - development of anti-CAVD treatment is urgent task. For better understanding of molecular mechanis...
ORGANISM(S): Homo sapiens (Human) 
2022-10-21 | PXD032212 | Pride
Osteogenic differentiation Q exactive Valve interstitial cells Cavd 
Sort   by:  
 Page size