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ProteomicsAIG1 and ADTRP are endogenous FAHFA hydrolases
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a newly discovered class of signaling lipids with anti-inflammatory and anti-diabetic activities. However, the endogenous regulation of FAHFAs remains a pressing but unanswered question. Here, we find that androgen- induced gene 1 (AIG1) and andr...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-09 | PXD017539 | Pride
Threonine hydrolase Metabolism Adtrp Lipid Enzyme inhibitor Fahfa Aig1 Diabetes 
ProteomicsCell-selective multiplexed bioorthogonal noncanonical amino acid tagging for versatile nascent proteome profiling
Cell-selective bioorthogonal noncanonical amino acid tagging (BONCAT) of newly synthesized proteins, catalyzed by engineered aminoacyl-tRNA synthetase (aaRSs), is a powerful technology for time-resolved characterization of the nascent proteome. Currently, cell-selective BONCAT studies in bacteria re...
ORGANISM(S): Klebsiella pneumoniae ATCC 43816 Escherichia coli Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2026-04-11 | PXD067013 | Pride
Chemoproteomics Boncat Nascent proteins 
ProteomicsExpedited mapping of the ligandable proteome using fully functionalized enantiomeric probe pairs
A fundamental challenge in chemical biology and medicine is to understand and expand the fraction of the human proteome that can be targeted by small molecules. We recently described a strategy that integrates fragment-based ligand discovery with chemical proteomics to furnish global portraits of re...
ORGANISM(S): Homo sapiens (Human) 
2019-10-29 | PXD015104 | Pride
Human Pbmcs Hek293t Lc-ms/ms 
ProteomicsMonitoring Fe-S cluster occupancy across the E. coli proteome using chemoproteomics
Iron-sulfur (Fe-S) clusters are ubiquitous metallocofactors involved in redox chemistry, radical generation, and gene regulation. Common methods to monitor Fe-S clusters include spectroscopic analysis of purified proteins, and auto-radiographic visualization of radiolabeled iron distribution in prot...
ORGANISM(S): Escherichia coli 
2024-05-22 | PXD026488 | Pride
Redime Iron-sulfur clusters Isotop-abpp E. coli 
ProteomicsHeterogeneous adaptation of cysteine reactivity to a covalent oncometabolite
Metabolism and signaling intersect in the genetic cancer syndrome hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a disease in which mutation of the TCA cycle enzyme fumarate hydratase (FH) causes hyperaccumulation of fumarate. This electrophilic oncometabolite can alter gene activity at...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD020073 | Pride
Human Cysteine reactivity Kidney Hlrcc Uok268 
ProteomicsChemoproteomics reveals disruption of metal homeostasis and metalloproteins by the antibiotic holomycin
The natural product holomycin contains a unique cyclic disulfide and exhibits broad-spectrum antimicrobial activities. Reduced holomycin chelates metal ions with high affinity and disrupts metal homeostasis in the cell. To identify cellular metalloproteins that are affected by holomycin, reactive-cy...
ORGANISM(S): Escherichia coli 
2023-08-14 | PXD040777 | Pride
Antibiotics Chemoproteomics Metalloproteins 
ProteomicsCharacterizing metabolic drivers of Clostridioides difficile infection with activity-based hydrazine probes
Many enzymes require post-translational modifications or cofactor machinery for primary function. As these catalytically essential moieties are highly regulated, they act as dual sensors and chemical handles for context-dependent metabolic activity. Clostridioides difficile is a major nosocomial pat...
ORGANISM(S): Clostridioides difficile ATCC 43255 
2023-03-11 | PXD039143 | Pride
Metabolism C. difficile Cofactor Hydrazine Abpp 
ProteomicsE. coli monothiol glutaredoxin GrxD replenishes Fe-S clusters to the essential ErpA A-type carrier under low iron stress  
Iron-sulfur (Fe-S) clusters are required for essential biological pathways, including respiration and isoprenoid biosynthesis. Complex Fe-S cluster biogenesis systems have evolved to maintain an adequate supply of this critical protein cofactor. In Escherichia coli, two Fe-S biosynthetic systems, th...
ORGANISM(S): Escherichia coli 
2024-07-13 | PXD053317 | Pride
Iron-sulfur (fe-s) protein Monothiol glutaredoxin grxd A-type carrier erpa Mass spectrometry (ms) E. coli Chemoproteomics Iron metabolism Metal homeostasis 
ProteomicsHydralazine inhibits cysteamine dioxygenase to treat preeclampsia and senesce glioblastoma
Many safe and effective drugs have unknown targets, limiting opportunities to extend their use to other disease states with overlapping etiologies. Here, we have elucidated the mechanism of action of the direct vasodilator, hydralazine (HYZ), which has been used for more than seven decades and remai...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-10-24 | PXD067316 | Pride
Glioblastoma Hydralazine Lc-ms Preeclampsia Mechanisms of action 
ProteomicsHuman cytomegalovirus-induced host protein citrullination is crucial for viral replication
Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifi...
ORGANISM(S): Homo sapiens (Human) 
2021-07-20 | PXD025818 | Pride
Citrullination 
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