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ProteomicsThermal proteome profiling of cell cycle
We combined quantitative mass spectrometry with thermal profiling to systematically analyze the thermal stability and solubility of proteins during the eukaryotic cell cycle on a proteome-wide scale.
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008646 | Pride
Complexes Disordered proteins Cell cycle Thermal stability 
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
ProteomicsThermal proteome profiling in Escherichia coli
We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-07-09 | PXD009495 | Pride
Target engagement Escherichia coli Drugs Growth phase Meltome Thermal proteome profiling 
ProteomicsIntegrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.
Altered thermal solubility measurement techniques are emerging as powerful tools to assess ligand binding, post-translational modification, protein-protein interactions, and many other cellular processes that affect protein state under various cellular conditions. Thermal solubility or stability pro...
ORGANISM(S): Escherichia coli 
2024-02-14 | PXD031800 | Pride
Pisa Thermal proteomic profiling 
ProteomicsIdentification of phosphosites that alter protein thermal stability
We developed a robust method termed Dali that identifies phosphorylation events that alter protein thermal stability as a proxy for function.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-18 | PXD016750 | Pride
Proteomics Function Thermal stability Phosphorylation Dali 
ProteomicsOff-target profiling of Olaparib using Thermal Proteome Profiling
Explore off-targets of Olaparib using 2D Thermal Proteome Profiling (2D-TPP)
ORGANISM(S): Homo sapiens (Human) 
2024-05-13 | PXD046614 | Pride
Thermal proteome profiling Parp 2d-tpp Olaparib 
ProteomicsThermal proteome profiling in of CA-074me
We performed thermal proteome profiling (TPP) to study changes to protein thermal stability and abundance of CA-074me (cathepsin inhibitor) in RAW264.7 macrophages after Salmonella typhimurium infection.
ORGANISM(S): Mus musculus (Mouse) Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
2020-02-07 | PXD016086 | Pride
Target engagement Cathepsin inhibitor Salmonella Thermal proteome profiling 
ProteomicsThermal proteome profiling and proteome analysis using high-definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next-generation galeterone analog VNPP433-3β in castration-resistant prostate cancer
Cholesterol (CHOL) homeostasis is significantly modulated in prostate cancer (PCa) suggesting an active role in PCa development and progression. Several studies indicate a strong correlation between elevated CHOL levels and increased PCa risk and severity. Inhibition of CHOL biosynthesis at differen...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD060338 | Pride
Next generation galeterone analog Cyp51a1 Thermal proteome profiling Cholesterol Prostate cancer Lanosterol synthase 
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