Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast)
Mapping the subcellular proteome using a differential fractionation and an orthogonal nycodenz fractionation combined with quantitative mass spectrometry using isobaric labeling (TMT) in MS3
Alternative splicing (AS) is a key regulatory process in eukaryotes. Despite its importance, the impact of AS on plant protein diversity and its global effect on the proteome remain largely unexplored. To address this, we included the AS acinus pinin mutant in our qualitative proteomic analysis to i...
This study presents a tandem mass tag (TMT)–based quantitative proteomic analysis of human platelets treated with the BTK degraders NX-2127 or NX-5948, or vehicle control (DMSO). Washed platelet lysates from three independent biological replicates per condition were digested with trypsin, labelled w...
This study examined global proteomic changes in postmortem hippocampus tissue from Non-Hispanic Whites and African American individuals who were cognitively normal or had Alzheimer's disease using LC-MS3 on an Orbitrap Fusion Lumos. Proteins were quantified in the LC-MS3 experiments using Tandem Mas...
FACS isolated human alpha and beta cells were analyzed by TMT 11-plex labeling, Off-line basic pH reversed phase fractionation and LC-MS3 on an Orbitrap Fusion mass spectrometer.
Significant progress has been observed in recent years in proteomics tools. However, minimal application of the newest proteomic tools has been massively applied in plant recalcitrant tissues in non-model fruits. This constant occurrence constrains the progress in understanding essential molecular p...
This study examined global proteomic changes in postmortem globus pallidus (GP) tissue from Non-Hispanic Whites and African American individuals who were cognitively normal or had Alzheimer's disease using LC-MS3 on an Orbitrap Fusion Lumos. Proteins were quantified in the LC-MS3 experiments using T...
Cross-linking mass spectrometry is a powerful method for the investigation of protein-protein interactions from highly complex samples. XL-MS combined with tandem mass tag labeling holds the promise of large-scale PPI quantification. However, a robust and efficient TMT-based XL-MS quantification met...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Bacteria
