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ProteomicsOptimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
In large-scale quantitative mass spectrometry (MS)-based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high-pH reversed-phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affects sensi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-28 | PXD031277 | Pride
Tandem mass tags Phosphoproteomics Optimization High-ph fractionation Isobaric labeling 
ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
ProteomicsQuantitative TMT phosphoproteomics of HEK293T cells with inducibly expressed OspF
This is a TMT phosphoproteomics dataset comparing doxycyline-induced vs. uninduced populations of HEK293T cells with OspF under a doxycycline-inducible promoter.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-08-12 | MSV000098806 | MassIVE
ProteomicsTMT-based proteomics and phosphoproteomics analysis of proteome changes in seminal plasma extracellular vesicles of azoospermia
The proteome and phosphoproteomics changes were quantified in seminal plasma extracellular vesicles among healthy individuals with normal sperm, nonobstructive azoospermia and obstructive azoospermia using TMT 10-plex by LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022843 | Pride
Tmt  azoospermia  extracellular vesicles  seminal plasma  proteomics 
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
UnknownLowering Sample Requirements to Study Tyrosine Kinase Signaling Using Phosphoproteomics with the TMT Calibrator Approach.
Not available
S-EPMC7771371 | biostudies-literature
ProteomicsTMT-10plex-based phosphoproteomics of bioactive compounds in A549 cells
We conducted a comprehensive screening of bioactive compounds sourced from natural products, specifically targeting those capable of inducing apoptosis while inhibiting tyrosine kinase activity. Our investigation encompassed a diverse range of compounds, including pure compounds, compound mixtures, ...
ORGANISM(S): Homo sapiens (Human) 
2024-03-13 | PXD050555 | Pride
Post translational modification Lc-ms/ms Phosphorylation Apoptosis Lung epidermal cells Isobaric mass tag Egfr 
UnknownTandem Mass Tag-10plex (TMT-10plex) phosphoproteomics dataset for comprehensive analysis of active compounds with tyrosine kinase inhibition activity.
Not available
S-EPMC11214987 | biostudies-literature
ProteomicsHiRIEF LC-MS allows for in depth quantitative analysis of the phosphoproteome.
In this project we aimed at improving phosphoproteomics analysis by taking advantage of high resolution isoelectric focusing (HiRIEF) fractionation. We developed a workflow that employs titanium dioxide phospho-enrichment, followed by isobaric labeling with Tandem Mass Tags (TMT) and HiRIEF on a bro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-07 | PXD005410 | Pride
Multiplex Cells Isoelectric point Tmt Phospho-peptides Phospho-sites Fractionation Quantification Networkin Cancer Phosphoproteomics Tyrosine Ipg Proteomics Hirief 10-plex Lc-ms Quantitative Mitosis Mitotic arrest Hela Kinase association Multiply phosphorylated Phosphorylation Pervanadate 
ProteomicsLowering Sample Requirements to Study Tyrosine Kinase Signaling using Phosphoproteomics with the TMT Calibrator Approach
Analysis of tyrosine kinase signaling is critical for the development of targeted cancer therapy. Currently, immunoprecipitation (IP) of phosphotyrosine (pY) peptides prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to profile kinase substrates. A typical protocol requests ...
ORGANISM(S): Homo sapiens (Human) 
2020-07-29 | PXD018952 | Pride
Signaling Tmt Cancer Calibrator Tyrosine phosphorylation 
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