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ProteomicsERK inhibits Cic repressor function via multisite phosphorylation
The receptor tyrosine kinase (RTK)/Extracellular Signal-Regulated Kinase (ERK) signaling pathway controls cell proliferation, differentiation, and survival. How ERK activation is relayed to its phosphorylation targets is not well understood. The transcriptional repressor Capicua (Cic) has emerged as...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2026-03-16 | PXD066179 | Pride
Erk Multisite phosphorylation Capicua Drosophila 
ProteomicsVap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase
To identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-02-01 | PXD035110 | Pride
Vap33 Embryo Drosophila 
ProteomicsNDR kinase Tricornered genetically interacts with Ccm3 and metabolic enzymes in Drosophila melanogaster tracheal development
The GckIII pathway is a Hippo-like kinase module defined by sequential activation of Ste20 kinases Thousand and One (Tao) and Germinal Center Kinase III (GckIII), followed by nuclear dbf2-related (NDR) kinase Tricornered (Trc). We previously uncovered a role for the GckIII pathway in regulating tube...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-03-13 | PXD038912 | Pride
Trachea Drosophila Ndr kinase Tricornered 
ProteomicsLgl protein interactome - Lgl reduces endosomal vesicle acidification and Notch signaling by promoting the interaction between Vap33 and the V-ATPase complex
To identify Lgl binding proteins, we purified Lgl-containing protein complexes from cultured Drosophila S2 cells, using the single-step streptavidin purification from stable cell lines expressing SBP-tagged Lgl, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2018-06-10 | PXD009568 | Pride
Lgl S2 cells Drosophila 
ProteomicsRegulation of Drosophila brain development and organ growth by the Minibrain/Rala signaling network
Using affinity purification-mass spectrometry (AP-MS), we identified the novel Mnb binding partners Ral interacting protein (Rlip) and RALBP1 associated Eps domain containing (Reps). Rlip and Reps physically and genetically interact with Mnb, and the three proteins may form a ternary complex. Mnb ph...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-09-09 | PXD052378 | Pride
Embryo Drosophila Minibrain 
ProteomicsYorkie and Bonus protein interactome-Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye
To identify novel regulators of the Hippo pathway, we performed affinity purification-mass spectrometry (AP-MS) using Drosophila embryos overexpressing Yki-EGFP with a ubiquitous driver da-GAL4, as well as cultured S2 cells expressing Yki-SBP. We identified the core Hippo pathway components, multipl...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-01-17 | PXD027934 | Pride
Embryo Drosophila Yki S2 cell Bon Lc-ms/ms 
ProteomicsDrosophila yellow-h, dopaminechrome tautomerase
We report the identification of dopaminechrome tautomerase (DPT) that biosynthesizes 5,6-dihydroxyindole from dopaminechrome in Drosophila. We show that this enzyme is encoded by the yellow-h gene.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-01-17 | PXD023295 | Pride
Dopamine Melanogenesis Dopaminechrome Drosophila Yellow-h Tautomerase Eumelanin 
GenomicsWhole genome sequencing of TF-AID-mutagenized flies
In this study, we stably expressed TF-AID in the germline of Drosophila melanogaster to induce mutations around specific transcription factor binding sites across the genome. To identify the mutations, we sequenced: (1) individual flies from the TF-AID-expressing populations, sampled across generati...
ORGANISM(S): Drosophila melanogaster 
2025-01-23 | E-MTAB-13796 | biostudies-arrayexpress
GenomicsWhole genome sequencing of experimentally evolved D. melanogaster populations with 4xBicoid
EMS-mutagenized D. melanogaster populations with 4xbicoid were sequenced at the 1st, 3rd, 7th, 9th and 15th generation to analyze genomic changes during experimental evolution. Non-mutagenized populations were sequenced in parallel, representing standing variations.
ORGANISM(S): Drosophila melanogaster 
2023-05-16 | E-MTAB-11768 | biostudies-arrayexpress
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