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ProteomicsDPP8/9 processing of human AK2 unmasks an IAP binding motif
AK2 is n-terminally processed by methionine aminopeptidases, N-terminal acetylation and DDP8/9 to become an active protein which is then sorted in the mitochondrial intermembrane space. We used peptide pull-downs of free and acetylated N-terminal peptides of AK2 from the different processing steps t...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD047178 | Pride
Ddp8/9 cleavage Peptide pull-downs Mass spectrometry N-terminal processing N-terminal acetlyation 
ProteomicsProteomic changes of Naa20 knockdown in Arabidopsis thaliana
N-terminal acetyltransferase B (NatB) acetylates around 20% of the eukaryotic proteome and is crucial for protein function and organismal development. However, the regulatory roles of NatB-mediated N-terminal acetylation (NTA) in proteome stability and autophagy regulation remain unclear in plants. ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-01 | PXD066676 | Pride
Naa20 Ubiquitination Plants 
ProteomicsCharacterization of the pocket mutant of cyclin B1
The APC complex was purified from insect cells and an in vitro phosphorylation purified WT cyclin B1 or pocket mutant cyclin B1 was performed. Phosphorylation sites were detected by mass spectrometry (in vitro data). WT cyclin B1 and mutant cyclin B1 was transfected in cells and the APC complex imm...
ORGANISM(S): Homo sapiens (Human) 
2025-04-03 | PXD047204 | Pride
Phosphorylation Cyclin b1 Pocket mutant cyclin b1 Immunoprecipitation Apc complex 
ProteomicsCUL4A-DDB1-DCAF10 is an n-recognin for Nt-acetylated Src kinases
cotranslational n-terminal modifications such as methionine excision, acetylation, and myristoylation contribute to protein stability, localization and three-dimensional folding. Disruption of these modifcations expose short n-terminal degron motifs that ultimately trigger protein degradation throug...
ORGANISM(S): Homo sapiens (Human) 
2025-12-04 | PXD061095 | Pride
N-t acetylation Sfks N-degron dcaf10 Ubiquitination Nt- myristoylation Cotranslational modifications N-recognin 
ProteomicsOverlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization
SMAC/DIABLO and HTRA2 are mitochondrial proteins whose N-termina sequences, known as inhibitor of apoptosis binding motifs (IBMs), bind and activate ubiquitin ligases knwon as inhibitor of apoptosis proteins (IAPs), unleashing a cell´s poteintial.IBMs comprise a 4-residue, loose consensus sequence, ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD021815 | Pride
N-terminal acetylation Birc3 Birc2 Natb N-terminomics Nata Apoptosis Xiap N-degron 
ProteomicsCross-linking mass spectrometry analyses of three different kinetochore protein complexes (KMN, NDC80C, MIS12C) using an MS-cleavable cross-linker, BuUrBu (DSBU)
In order to develop a simplified cross-linking mass spectrometry protocol, we applied one-step size-exclusion chromatography (SEC) for peptide purification after proteolysis with Lys-C and trypsin. Three benchmark protein complexes (KMN, NDC80C, MIS12C) from human kinetochore were cross-linked with ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-01 | PXD010070 | Pride
Dsbu Q-exactive Cross-linking ms Buurbu Merox Human Kinetochore Protein complex Ms-cleavable cross-linker 
ProteomicsBiochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates
Crossing over between homologs is critical for the stable segregation of chromosomes during the first meiotic division. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of the majority of crossovers during meiosis. Here we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-07-20 | PXD039839 | Pride
Crossover Yeast Phospho proteomics Meiosis Mer3 
ProteomicsAlternative polyadenylation factor CPSF6 regulates temperature compensation of the mammalian circadian clock
A defining property of circadian clocks is temperature compensation, characterized by the resilience of circadian free-running periods against changes in environmental temperature.We quantify global changes in 3´UTR length as well as gene and protein expression between wild type and CPSF6 knock-down...
ORGANISM(S): Homo sapiens (Human) 
2023-05-23 | PXD029343 | Pride
Eukaryotic translation initiation factor 2 subunit 1 Integrative proteomics Cleavage and polyadenylation specificity factor subunit 6 Circadian clock Temperature compensation Alternative polyadenylation 
ProteomicsTitle: Non-canonical functions of SNAIL drive context-specific progression
SNAIL is a key transcriptional regulator in embryonic development and cancer. Its effects in physiology and disease are believed to be linked to its role as a master regulator of epithelial-to-mesenchymal transition (EMT). Here, we report EMT-independent oncogenic SNAIL functions in cancer. Using ge...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-13 | PXD038726 | Pride
Transcription Emt Tissue specificity Snail Cell cycle : cancer Chip-ms 
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