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ProteomicsHipSci: the iPSC proteomic compendium
Human induced pluripotent stem cell (iPSC) technology holds great potential for therapeutic and research purposes, by providing improved models of disease and for studying mechanisms regulating differentiation. The Human Induced Pluripotent Stem Cell Initiative (HipSci) was established to generate l...
ORGANISM(S): Homo sapiens (Human) 
2019-07-25 | PXD010557 | Pride
Dda Ipsc Proteomics Homo sapiens Human Orbitrap Fusion Stem cells Tmt 
ProteomicsDefining the T cell proteome and the role of mTORC1 during differentiation using quantitative proteomics
High-resolution quantitative mass-spectrometry reveals similarities and disparities in how murine naïve CD4 and CD8 T cells respond to immune activation and re-structure proteomes as they differentiate to effector cells. The data map core transcriptional, metabolic and protein synthesis machinery, n...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-28 | PXD012058 | Pride
Mouse T cell Mtorc1 Fusion Tmt Mtor Rapamycin 
ProteomicsTissue adaptation is the dominant driver of the proteomic landscape of intestinal intraepithelial lymphocytes
Intestinal intraepithelial T lymphocytes (T-IEL) patrol the single layer of epithelial cells lining the gut, and consist of both induced T-IEL, derived from systemic antigen-experienced lymphocytes, and natural IEL, that are developmentally targeted to the intestine. To gain functional insights into...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-15 | PXD023140 | Pride
Tmt T cells Lymph nodes Lymphocytes Iel 
ProteomicsNrf2 is a central regulator of the metabolic landscape in macrophages and finetunes their inflammatory response
To overcome oxidative, inflammatory, and metabolic stress, cells have evolved networks of cytoprotective proteins controlled by transcription factor nuclear factor erythroid 2 p45-related factor 2 (Nrf2) and its main negative regulator the Kelch-like ECH associated protein 1 (Keap1). Here, we used h...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-13 | PXD027737 | Pride
Tmt Keap1 Nrf2 Mitochondria Bmdm Murine 
ProteomicsDIA proteomic analysis of unstimulated murine bone marrow derived macrophages from WT, Nrf2 KO and Keap1 KD cells
Unstimulated murine bone marrow derived macrophages cultured in L92 media from WT (4 biological replicates), Nrf2 KO (3 biological replicates) and Keap1 KD BMDM (3 biological replicates) were processed and analysed utilising DIA (label free) proteomic analysis. The Nrf2 KO mouse (DOI: 10.1006/bbrc.1...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-01 | PXD030455 | Pride
Dia Keap1 Nrf2 Macrophages Bmdm Murine 
ProteomicsAPEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
Fip200 Mitophagy Autophagy Frb-rapalog-fkbp interaction Ulk1 Apex2 Er 
ProteomicsSuper-killer CTLs are generated by single gene deletion of Bach2
Bach2 codes for a transcriptional regulator exerting major influences on T cell mediated immune regulation. Effector CTLs derived from in vitro activation of murine CD8+ T cells showed increased proliferative and cytolytic capacity in the absence of BACH2. Before activation, BACH2-deficient CD8+ T c...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-02 | PXD036457 | Pride
T cell Proteomics Bach2 Cd8 Ctl Tmt 
ProteomicsImmunological Proteome Resource (ImmPRes): Liver derived CD4+ T cell proteomes
T cells play a critical role in liver immunity and take part both in the initiation and in the resolution of intrahepatic inflammation. The liver contains conventional CD4 T cells, and Natural Killer T (NKT) cells that express an invariant Vα14 T cell receptor that recognizes glycolipid/CD1d antig...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-26 | PXD036319 | Pride
Cd4 Liver T cell Nkt Tmt 
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsHuman CD8 T cell IL2 deprivation time course
IL2 deprivation time course for day 8 human CD8 T cells activated for 3 days with TransAct T-cell activation beads. PBMC (Cambridge Bio) were defrosted and CD8+ T-cells isolated using a human CD8+ T-cell isolation kit. CD8+ T-cells were activated at 1 x 106 cells/mL with 1 in 100 TransAct T-cell ac...
ORGANISM(S): Homo sapiens (Human) 
2024-10-10 | PXD056475 | Pride
Dia Human Cd8 T cells 
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