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ProteomicsCharacterization of the pocket mutant of cyclin B1
The APC complex was purified from insect cells and an in vitro phosphorylation purified WT cyclin B1 or pocket mutant cyclin B1 was performed. Phosphorylation sites were detected by mass spectrometry (in vitro data). WT cyclin B1 and mutant cyclin B1 was transfected in cells and the APC complex imm...
ORGANISM(S): Homo sapiens (Human) 
2025-04-03 | PXD047204 | Pride
Phosphorylation Cyclin b1 Pocket mutant cyclin b1 Immunoprecipitation Apc complex 
TranscriptomicsGene expression data following Cyclin T2 and Cyclin T1 depletion by shRNA in HeLa cells
We have characterized gene expression changes in HeLa cells following long term depletion of Cyclin T2 or Cyclin T1 using shRNA HeLa cells were transduced with VSV-G pseudotyped lentiviral vectors expressing shRNA against either Cyclin T2 or Cyclin T1. As a control, cells were transduced with shRNA...
ORGANISM(S): Homo sapiens 
2011-08-02 | E-GEOD-28339 | biostudies-arrayexpress
ProteomicsSpatial control of the APC/C ensures the rapid degradation of Cyclin B1
The proper control of mitosis depends on the ubiquitin-mediated degradation of the right mitotic regulator at the right time. This is under the control of the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase that is regulated by the Spindle Assembly Checkpoint (SAC). The Checkpoint prev...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052677 | Pride
Apc/c Cyclin b1 
ProteomicsPositively charged specificity site in cyclin B1 is essential for mitotic fidelity
Phosphorylation of substrates by cyclin-dependent kinases (CDKs) is the driving force of cell cycle progression. Several CDK-activating cyclins are involved, yet how they contribute to substrate specificity is still poorly understood. Here, we discovered that a positively charged pocket in cyclin B1...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD048838 | Pride
Cyclin b Phosphate-binding pocket Mitosis Spindle Cdk1 Apc/c Ube2s 
ProteomicsSpatial control of the APC/C ensures the rapid degradation of Cyclin B1
The proper control of mitosis depends on the ubiquitin-mediated degradation of the right mitotic regulator at the right time. This is under the control of the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase that is regulated by the Spindle Assembly Checkpoint (SAC). The Checkpoint prev...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD046458 | Pride
Ubiquitin-mediated degradation Mitosis Spindle assembly checkpoint Human 
ProteomicsCyclin B1-Cdk1 binds MAD1 and facilitates its release from the nuclear pore complex to ensure a robust Spindle Assembly Checkpoint
How the cell rapidly and completely reorganises its architecture when it divides is a problem that has fascinated for almost 150 years. We now know that the core regulatory machinery is highly conserved in eukaryotes but how these multiple protein kinases, protein phosphatases, and ubiquitin ligases...
ORGANISM(S): Homo sapiens (Human) 
2020-06-29 | PXD017202 | Pride
Human Ms3 Tmt 
ProteomicsCDK5-Cyclin B1 regulates mitotic progression and fidelity
Mammalian cell cycle is driven by several CDK-cyclin complexes, with CDK1/cyclin B1 (CCNB1) being the one that is essential for mitotic entry and progression. CDK5 is not considered a typical CDK due to its high expression in post-mitotic neurons and activation by non-cyclin proteins p35 and p39. We...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD038386 | Pride
Human Phosphopeptides Rpe1 Imac Tmt 1nm-pp1 Cdk5 Dtag-13 Brp fractionation Cell line Lc-ms/ms 
ProteomicsTargeting G1–S Checkpoint–Compromised Cancers with Cyclin A/B RxL Inhibitors
Cancer cell proliferation requires precise control of E2F1 activity; excess activity promotes apoptosis. Here, we developed cell-permeable and bioavailable macrocycles that selectively kill small cell lung cancer (SCLC) cells with inherent high E2F1 activity by blocking RxL-mediated interactions of ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-30 | PXD055112 | Pride
Macrocycle Cyclin a E2f Cyclin b Cy/rxl motif Cdk2 Sac Synthetic lethality 
ProteomicsCyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses
Mitosis in early embryos often proceeds at a rapid pace, but how this pace is achieved is not understood. Here we show that cyclin B3 is the dominant driver of rapid embryonic mitoses in the C. elegans embryo. Cyclins B1 and B2 support slow mitosis (NEBD to Anaphase ~600s) but the presence of cyclin...
ORGANISM(S): Caenorhabditis elegans 
2024-07-29 | PXD053811 | Pride
Mitosis Meiosis Cdk1 Cell division Cyclin b1 Cdc20 Cyclin b3 Apc/c Cyclin b2 Embryonic development 
GenomicsCyclin B1 deficiency in renal tubular epithelial cells promotes kidney ageing and fibrosis in mice
Cyclin B1 deficiency in renal tubular epithelial cells promotes kidney ageing and fibrosis in mice
PRJNA929467 | ENA
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