Sort   by:  
 Page size 
ProteomicsA CRISPR/Cas9-mediated screen identifies regulators of early plasma cell differentiation
The differentiation of B cells into antibody-secreting cells depends on cell division-coupled, epigenetic and other cellular processes that are incompletely understood. We have developed a CRISPR/Cas9-based screen that models an early stage of T cell-dependent plasma cell differentiation and measure...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-22 | PXD038201 | Pride
Plasmablasts Screen Plasma cell differentiation Crispr/cas9 Regulators Gene knockout Antibody-secreting cells Plasma cells and antibody secretion 
ProteomicsProteomics of serotonergic autoregulation
Serotonin regulates its own production, degradation and release in the Raphe nuclei of the brain mainly by modifying the level and activity of autoreceptors on serotonin-producing neurons, such as 5-HT1A and 5-HT1B. This autoregulation is probably the reason for the delayed response of patients with...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD055579 | Pride
Serotonin Serotonin transporter Brain Tryptophan hydroxylase Autoregulation Mouse 
ProteomicsB-cell-specific MYD88 L252P expression causes a premalignant gammopathy resembling IgM MGUS
A highly recurrent, somatic L265P mutation in the TIR domain of the signaling adapter MYD88 constitutively activates NF-kB. It occurs in nearly all human patients with Waldenström’s macroglobulinemia (WM), a B cell malignancy caused by IgM-expressing cells. Here we introduced an inducible leucine to...
ORGANISM(S): Mus musculus (Mouse) 
2020-11-06 | PXD017292 | Pride
Lymphoid neoplasia 
ProteomicsNeurofibromatosis type 1-dependent alterations in mouse microglia function are not cell-intrinsic
We previously discovered a sex-by-genotype defect in microglia function using a germline heterozygous knockout mouse model of Neurofibromatosis type 1 (Nf1+/- mice), in which only microglia from male Nf1+/- mice exhibited defects in purinergic signaling. Herein, we leveraged an unbiased proteomic ap...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD035881 | Pride
Purinergic signaling Sex differences Neurofibromin Nf1 Cortex Microglia Genetically engineered mice 
ProteomicsA long-term 3D organoid (tubuloid) model of the adult mouse kidney
The aim of the project was the analysis of the bulk proteome and phosphoproteome in 3D organoids (tubuloids) from the adult mouse kidney. Proteome (nearly 9000 proteins) and phosphoproteome (nearly 16000 phospho sites) were compared in three biological (independent) replicates of epithelial organoid...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-26 | PXD023491 | Pride
Proteomics Organoids 
ProteomicsSex-Specific Microglia Defects in the Neuroligin-4 Knock-Out Mouse Model of Autism Spectrum Disorder
Neuroligin-4 (NL4) loss-of-function mutations are strongly associated with monogenic heritable abnormalities linked with Autism Spectrum Disorder (ASD). NL4 mutation in mice causes ASD related alterations in both, the synaptic and behavioral phenotype. Since microglia closely regulate synaptic devel...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-24 | PXD024325 | Pride
Sex difference Gamma oscillation Estrogen Microglia Autism spectrum disorder 
ProteomicsProtection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region
RIF1 is a multifunctional protein that plays key roles in the regulation of DNA processing. During repair of DNA double-strand breaks (DSBs), RIF1 functions in the 53BP1-Shieldin pathway that inhibits resection of DNA ends to modulate the cellular decision on which repair pathway to engage. Under co...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-14 | PXD031972 | Pride
Sq motifs Rif1 Dsb resection inhibition Dna replication fork protection Intrinsically disordered region 
ProteomicsNovel inhibitor(s) of NO-production for microglia and macrophages
We aimed to identify novel therapeutic compounds (small molecules) to target microglia and macrophage NO production. We set up a screen using a library of 30.000 drug-like compounds in collaboration with the FEM. Using a microglia cell line and primary microglia and macrophages, we identified one co...
ORGANISM(S): Mus musculus (Mouse) 
2022-01-24 | PXD020613 | Pride
Compound ms Mouse Lc-ms Small molecule Exploris 480 No-synthesis 
ProteomicsUSP15 deubiquitinase safeguards hematopoiesis and genome integrity in hematopoietic stem cells and leukemia cells
To decipher the role of USP15 in DNA damage in leukemia cells, endogenous USP15 was immunoprecipitated from MV4-11 and Kasumi-1 cell lines under naive and DNA stress conditions (Mitomycin C, 30nM, 1h). 355 candidates co-immunoprecipitated with USP15 in all the conditions, in 4 independent experiment...
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD020612 | Pride
Human Dna damage Ubiquitin 
ProteomicsDistinct roles for MNK1 and MNK2 in social and cognitive behavior through kinase-specific regulation of the synaptic proteome and phosphoproteome
Local mRNA translation is required for adaptive changes in the synaptic proteome. The mitogen-activated protein kinase (MAPK) interacting protein kinases 1 and 2 (MNK1 and MNK2) have emerged as key regulators of neuronal translation, primarily through phosphorylation of the eukaryotic initiation fac...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-01 | PXD058409 | Pride
Mnk1 Tmt Synaptoneurosomes Mnk2 
Sort   by:  
 Page size