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ProteomicsIdentifying peptides and sites involved in FtsZ self-assembly in Escherichia coli through in vivo protein photocrosslinking of FtsZ residues mediated by the unnatural amino acid Bpa (p-benzoyl-L-phenylalanine), followed by tandem mass spectrometry analysis of purified FtsZ crosslinking dimers.
Bacterial cell division hinges on the Z-ring, an architecture built from the dynamical assembly and disassembly of FtsZ proteins. This delicate balance ensures not only apparent stability, but also continuous remodeling, both of which are required for Z-ring functioning. However, the molecular natur...
ORGANISM(S): Escherichia coli 
2025-05-10 | PXD063736 | Pride
Escherichia coli Ftsz Bacterial cell division P-benzoyl-l-phenylalanine Photocrosslinking 
ProteomicsThe Hsp90 molecular chaperone governs client proteins by targeting intrinsically disordered regions
Molecular chaperones govern proteome health to support cell homeostasis. An essential eukaryotic component of the chaperone system is Hsp90. Using a chemical-biology approach we characterized the features driving the Hsp90 physical interactome.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-08-10 | PXD040875 | Pride
Hsp90 Interactome Crosslinking Bpa 
ProteomicsIdentification of Cross-linking partners of BpA modified p97 by photo cross-linking MS
We wanted to see where and how the protein p97 interacts with its partner proteins p37, PPI,Sds22 and I3. To this end a p97 derivative with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) was generated. After incubation with the partner proteins, photocross-linking and trypti...
ORGANISM(S): Homo sapiens (Human) 
2023-07-10 | PXD039939 | Pride
Aaa protease Bpa crosslinking P97 
ProteomicsA High-Performance Workflow for Identifying Site-Specific Crosslinks Originating from a Genetically Incorporated, Photoreactive Amino Acid
In conventional crosslinking mass spectrometry, proteins are crosslinked using a highly selective, bifunctional chemical reagent, which limits crosslinks to residues that are accessible and reactive to the reagent. Genetically incorporating a photoreactive amino acid offers two key advantages: any s...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-06-11 | PXD050493 | Pride
Human small heat shock proteins Benzoylphenylalanine Thermo orbitrap fusion lumos tribrid Crosslinking 
ProteomicsInteraction of Hsp70 Ssb1 and atypical Hsp70 Ssz1
Our goal was to better understand the interaction between the Hsp70 Ssb1 and the atypical Hsp70 Ssz1, which we had uncovered by in vivo site-specific Bpa crosslinking. Ssz1 having Bpa incorporated was purified and incubated with purified Ssb1 and the J-domain protein Zuo1 during activation of photo ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-08-26 | PXD024065 | Pride
Yeast Ssb1 Mass spectrometry Photo crosslinking Ssz1 
ProteomicsNovel recognition site for polyUb and UBL signals in Rpn1
Rpn1 is the largest proteasomal receptor for polyubiquitin and shuttle proteins contains one known binding site for polyubiquitin and shuttle proteins. We used a combination of NMR spectroscopy, photo-crosslinking, mass spectrometry, and mutagenesis,to confirm the presence of additional ubiquitin re...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2021-08-09 | PXD027128 | Pride
Uv-crosslinking Rpn1 Bpa Ubiquitin 
ProteomicsAn UBX domain in UBXD1 organizes Ub binding at the C-terminus of the VCP/p97 AAA-ATPase
Identification of crosslinked peptides between UBXD1 and HR23b using different cross-linking approaches (chemical cross-linking with DSSO and photo crosslinking with incorporated BPA).
ORGANISM(S): Homo sapiens (Human) 
2023-05-25 | PXD040984 | Pride
Hr23b Ubxd1 Dsso Ubx domain Ubiqutin Bpa Clms 
ProteomicsAn UBX domain in UBXD1 organizes Ub binding at the C-terminus of the VCP/p97 AAA-ATPase
Identification of crosslinked peptides between UBXD1, Ubiquitin and p97 using different cross-linking approaches (chemical cross-linking with DSSO, photo crosslinking with incorporated BPA or photo-Met)
ORGANISM(S): Homo sapiens (Human) 
2023-05-25 | PXD039606 | Pride
Ubxd1 Photo-met Dsso P97/vcp Ubx domain Ubiqutin Bpa Clms 
ProteomicsChemical and photo-Crosslinking experiments of the BAX/BNIP3/PeptideB interaction
In these experiments we try to show by cross-linking MS how BAX and BNIP3 interact (DSSO cross-linking) and how Peptide B (an octapeptide that prevents mitochondrial damage and cell death induced by BNIP3/BAX activity) interacts with the BNIP3/BAX complex (BPA cross-linking; BPA on Peptide B).
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD056758 | Pride
Bnip3 Bax Mitochondrial damage Cell death 
ProteomicsLC-MS/MS analysis of Prp28-K136BPA experiment
We use the BPA crosslinker in Prp28 to “capture” Prp28 in action within the assembling splicing complexes by UV crosslinking. We wish to explore the interacting targets of Prp28-K136BPA within the assembling splicing complexes.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-04-20 | PXD024492 | Pride
Lc-msms Yeast Prp28 
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