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ProteomicsExploring the virulence gene interactome with CRISPR/dCas9 in the human malaria parasite
The var multigene family encodes clonally variant surface antigens that are key to immune evasion and pathogenesis in the human malaria parasite, Plasmodium falciparum. Epigenetics and nuclear organization regulate the var gene family in a system of mutually exclusive expression; however, few factor...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2020-07-15 | PXD013131 | Pride
Malaria Dcas9 Crispr Interactome 
ProteomicsC-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-07 | PXD009216 | Pride
Apex2 Biotinylation Centromere Proteomes Cas9 Subnuclear Telomere 
ProteomicsdCas9 targeted chromatin and histone enrichment for mass spectrometry (Catchet-MS) identifies IKZF1 as a novel target for HIV-1 latency reversal
HIV-1 infected patients virally suppressed by antiviral treatment harbor a persistent reservoir of replication competent latent HIV-1 infected cells, which constitute the main roadblock to a cure. A main strategy for HIV cure aims to stimulate viral gene expression in latently infected cells so that...
ORGANISM(S): Homo sapiens (Human) 
2022-05-31 | PXD032975 | Pride
Catchet-ms Ikzf1 Hiv-1 latency reversal 
ProteomicsProteome of Cas9- and dCas9-expressing Lucilia cuprina larvae reared on vs. off doxycycline
We analyzed the proteomes of transgenic Cas9- and dCas9-expressing larvae of the Australian sheep blowfly, Lucilia cuprina. We measured abundance of red fluorescent protein (dsRed) in our samples in order to test Cas9 and dCas9 activity. Flies from two transgenic strains, DR7#2;Cas9-ryt and DR7#2;dC...
ORGANISM(S): Lucilia cuprina 
2026-04-27 | PXD071864 | Pride
Insect Blowfly Diptera Tet-off 1d-lc Dcas9 Cas9 Orbitrap astral Calliphoridae Dda Lucilia 
GenomicsTracking of dCas9-methyltransferase footprints
Tracking of dCas9-methyltransferase footprints
PRJNA347354 | ENA
GenomicsdCas9 activation of RP11-326A19.4
dCas9 activation of RP11-326A19.4
PRJNA548121 | ENA
GenomicsEffects of dCas9-demethylase chimeras in vivo
Effects of dCas9-demethylase chimeras in vivo
PRJNA1235646 | ENA
TranscriptomicsInhibition of uPA expression by CRISPR-dCas9 DNA methyltransferases
We report the generation of CRISPR-dCas9 DNA methyltransferases to mediate targeted DNA methylation. Using the dCas9-BFP-DNMT3A and dCas9-BFP-DNMT3B methyltransferases, we have demonstrated that these two methyltransferase can mediate targeted methylation in three human genes tested: uPA, TGFBR3, an...
ORGANISM(S): Homo sapiens 
2016-06-01 | E-GEOD-74935 | biostudies-arrayexpress
ProteomicsChIP-MS reveals the local chromatin composition by label-free quantitative proteomics
Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combined ChIP with label-free quantitative (LFQ) mass...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-03-05 | PXD039627 | Pride
Chip-ms Dcas9 Telomeres Lfq Myb 
ProteomicsValidation of α-satellite-associated regulators using the dCas9-TurboID system in HeLa cells
To validate whether the dCas9-TurboID system is applicable for proximity labeling at specific genomic regions, label-free mass spectrometry was employed to validate the satellite relative proteins that were enriched in the group of stable Hela cell line expressing sgAS.
ORGANISM(S): Homo Sapiens 
2025-03-05 | PXD061518 |
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