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ProteomicsIdentification of proteins that interact with TRAF6 in ADP-heptose- or IL-1β-stimulated HEK293 cells
The bacterial metabolite ADP-heptose activates the mammalian protein kinase ALPK1 (alpha protein kinase 1), which then phosphorylates TIFA (TRAF interacting protein with FHA domain). This leads to the oligomerisation of TIFA and the recruitment and oligomerisation of TRAF6 (TNF receptor-associated f...
ORGANISM(S): Homo sapiens (Human) 
2022-09-14 | PXD034964 | Pride
Adp-heptose Traf6 Tifa Alpk1 
ProteomicsPhospho-regulation of Cdc48/p97 cofactor protein Shp1/p47 in budding yeast
Shp1/p47 is a cofactor of the Cdc48/p97 complex involved in the energy-dependent segregation of intracellular aggregates and multiprotein complexes. We identified two serine residues (S108 and S315) in the S. Cerevisiae protein Shp1 which increase in phosphorylation upon various cell stresses such a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-30 | PXD035819 | Pride
Ubx domain Stress Phosphorylation Torc1 Protein degradation Ubx1 Proteostasis 
ProteomicsNRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress - Figure 1D/S1C
Please refer to the manuscript for a detailed description.
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD062616 | Pride
Osmotic stress Wnk pathway Nrbp1 pseudokinase 
ProteomicsNRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress - Figure 5B / 6A
Please refer to the manuscript for a detailed description.
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD062619 | Pride
Osmotic stress Wnk pathway Nrbp1 pseudokinase 
ProteomicsHuman UFSP1 is an active protease that regulates UFM1 maturation and UFMylation
An essential first step in the posttranslational modification of proteins with UFM1, UFMylation, is the proteolytic cleavage of pro-UFM1 to expose a C-terminal glycine. Of the two UFM1-specific proteases (UFSPs) identified in humans, only UFSP2 is reported to be active since the annotated sequence o...
ORGANISM(S): Homo sapiens (Human) 
2022-08-22 | PXD035142 | Pride
Ribosome Ufm1 Endoplasmic reticulum (er) Cysteine protease Ubiquitin like modifier 
ProteomicsSubstrates of the ATR kinase - Phosphoproteomics
Identification and validation of substrates of the ATR kinase by quantitative phosphoproteomics
ORGANISM(S): Homo sapiens (Human) 
2024-01-15 | PXD041250 | Pride
Phosphoproteomics Kinase Atr 
ProteomicsDirect ionic stress sensing and mitigation by the transcription factor NFAT5
Homeostatic control of intracellular ionic strength is essential for protein, organelle and genome function, yet mechanisms that sense and enable adaptation to ionic stress remain poorly understood in animals. We find that the transcription factor NFAT5 directly senses solution ionic strength using ...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-17 | PXD052591 | Pride
Hypertonic stress Proximity biotinylation Biomolecular condensates Ionic stress Intrinsically disordered protein Nfat5 Tonebp Phase separation 
ProteomicsDiGly analysis of autoubiquitinated UBR4 (residues 4730-5183)
Human recombinant UBR4 protein, consisting of residues 4730-5183, undergoes in vitro autoubiquitination when incubated with the E2 conjugating enzyme UBE2A and the other reaction components. The autoubiquitinated protein was analysed by mass spectrometry to identify modification sites and the ubiqui...
ORGANISM(S): Homo sapiens (Human) 
2023-11-15 | PXD046899 | Pride
Autoubiquitinated Ubr4 
ProteomicsIdentification of proteins interacting with the NEK1-C21ORF2 complex
We and others have found that the NEK1 kinase interacts with the C21ORF2 protein. Gel filtration indicated there may be other proteins in the complex. We tried to identify such proteins by immunoprecipitating endogenous NEK1 from cells and subjecting the precipitates to mass spectrometry. We also im...
ORGANISM(S): Homo sapiens (Human) 
2023-05-09 | PXD036410 | Pride
C21orf2 Dna repair Ciliogenesis Nek1 Kinase 
Proteomicsproteomics analysis of SDE2UBL base activity based probe immunoprecipitation
SDE2, a highly conserved and essential gene required for genome maintenance, coordinates essential processes ranging from pre-mRNA splicing and ribosome biogenesis to DNA repair and replication stress response. It is expressed as a full-length precursor (SDE2FL,) bearing an N-terminal ULD, which is ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-24 | PXD064155 | Pride
Ubiquitin-like fusion proteins Sde2 Activity-based probe Proteolysis Immunoprecipitation 
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