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ProteomicsC-Man peptide pulldowns from Brain
Peptide pulldowns with C-man antibodies to demostrate the utelity of reagents for proteomic analysis. Using Murine brain samples Novel C-man sites are readily identifiable.
ORGANISM(S): Mus musculus (Mouse) 
2020-11-16 | PXD020336 | Pride
C-man Affinity pulldowns Glycopeptides 
ProteomicsCombined Peptide array screen and proximity labeling proteomics fine map C/EBPa protein interactions
The pioneering transcription factor C/EBPα coordinates cell fate and cell differentiation. The structure of C/EBPα comprises intrinsically disordered regions, multiple short linear motifs, and extensive post-translational side chain modifications (PTM), reflecting its modularity and functional plast...
ORGANISM(S): Homo sapiens (Human) 
2021-07-12 | PXD022903 | Pride
Ptm Cebpa Bioid Peptide pulldowns 
ProteomicsEvaluation of peptide pulldown coupled to mass spectrometry approaches for investigation of motif-based protein-protein interactions
Eight different, SLiM-containing peptides were tested for pulldowns immobilized on magnetic beads, and the same sequences were used for PRISMA experiments. Double repeat of peptides were also used for higher local concentration of the motifs.
ORGANISM(S): Homo sapiens (Human) 
2023-03-07 | PXD037380 | Pride
Ap-ms Prisma Pulldown Slim Protein-protein interaction 
ProteomicsLRP peptide pulldowns - LRP-1 peptide, 4467-4481 as bait, in solution digests
LRP-1 peptide, 4467-4481 as bait, in solution digests
ORGANISM(S): Rattus norvegicus (Rat) 
2009-12-01 | PRD000143 | Pride
ProteomicsBANC-seq to identify genome-wide transcription factor binding affinities to native chromatin
BANC-seq (Binding Affinities to Native Chromatin by sequencing) allows determination of absolute apparent binding affinites of transcription factors to native chromatin in a genome-wide manner. In this study, we establish the method and show that chromatin and DNA sequence define binding affinites o...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-02-09 | PXD038502 | Pride
Dna pulldowns Transcription factors Tmt Proteomes Binding affinity Absolute quantification 
ProteomicsLimited-proteolysis coupled to mass spectrometry (LiP-MS) data on a serially filtered yeast lysate (the FLiP library) and on lysates of wild-type and Gcn5 mutant yeast strains grown under hydroxyurea stress and control conditions. Affinity purification mass spectrometry on Ada3 pulldowns from yeast grown under hydroxyurea stress and control conditions.
We applied serial ultrafiltration and limited proteolysis-coupled mass spectrometry (LiP-MS) to generate an atlas of peptides that act as markers for the formation or dissolution of protein-protein interactions (PPIs). Applied to Saccharomyces cerevisiae, the approach yielded 6,441 candidate PPI mar...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-17 | PXD055566 | Pride
Ap-ms Flip-ms Hydroxyurea Ada3 Lip-ms 
ProteomicsChemoproteomics of an NSD2-PWWP1 Chemical Probe
Here we use competitive chemoproteomics pulldowns followed by label-free quantitative LC-MS/MS to assess target engagement and selectivity profiles of UNC6934 and UNC7145, a chemical probe targeting the PWWP1 domain of NSD2 and its negative control counterpart, respectively. To this end, we used a b...
ORGANISM(S): Homo sapiens (Human) 
2021-08-06 | PXD017641 | Pride
Epigenetics Chemoproteomics Histone methyltransferase Chemical probe 
ProteomicsPlasmodium falciparum bromo-domain containing epigenetic protein complexes recruited to modified histone tails as identified by histone peptide pulldown (HPP)-MS/MS and further characterized by GFP-IP-MS/MS and HA-IP-MS/MS
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
Epigenetic complex Bromo-domain protein Hpp Gcn5 Gfp-ip Histone peptide pulldown Interactor Plasmodium falciparum Taf1 Histone acetylation Ha-ip Reader complex Histone methylation 
ProteomicsIdentification of novel RHOA interacting patners
RHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many...
ORGANISM(S): Homo sapiens (Human) 
2016-11-01 | PXD004934 | Pride
Silac  lc-ms  protein-protein interactions  rhoa 
ProteomicsProteomic identification of phosphorylation dependent Septin 7 interactors that drive dendritic spine formation
Protein from P15 mouse brain lysate was incubated with synthetic peptide corresponding to Septin 7 C-terminal tail residues 416-438. Either the phosphorylated (pT426) and non-phosphorylatied peptide was used for immunoprecipitation. Five replicate pulldowns were done per condition.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-01-19 | MSV000088699 | MassIVE
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