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ProteomicsConformational rearrangements upon start codon recognition in human 48S translation initiation complex
Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD031985 | Pride
Ribosome 48s initiation comples Eif1 
ProteomicsHsp multichaperone complex buffers pathologically modified Tau
Alzheimer's disease is a neurodegenerative disorder in which misfolding and aggregation of pathologically modified Tau is critical for neuronal dysfunction and degeneration. The two central chaperones Hsp70 and Hsp90 coordinate protein homeostasis, but the nature of the interaction of Tau with the H...
ORGANISM(S): Homo sapiens (Human) 
2022-08-11 | PXD032037 | Pride
Tau Hsp Multichaperone Alzheimer´s disease 
ProteomicsTransportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein
The nitric oxide synthase interacting protein (NOSIP), an E3-ubiquitin ligase, is involved in various processes like neuronal development, craniofacial development, granulopoiesis, mitogenic signaling, apoptosis and cell proliferation. The best characterized function of NOSIP is the regulation of eN...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033966 | Pride
Transportin Nuclear import 
ProteomicsCas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair
CRISPR-Cas constitutes an adaptive prokaryotic defence system against invasive nucleic acids like viruses and plasmids. Beyond their role in immunity, CRISPR-Cas systems have been shown to closely interact with components of cellular DNA repair pathways either by regulating their expression or via d...
ORGANISM(S): Haloferax volcanii DS2 
2022-05-20 | PXD029952 | Pride
Crispr Fen1 Archaea Dna damage Cas1 
ProteomicsDithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions
In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) nativ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2015-10-13 | PXD002656 | Pride
Dtt 
ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like pr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD034656 | Pride
Affinity purification Rna-processing Lfq Npl3 Spliceosome 
ProteomicsStructure of transcription preinitiation complex engaged with the +1 nucleosome
The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles in chromatin. In the yeast Saccharomyces ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD029840 | Pride
+1 nucleosome Transcription Preinitiation complex Tfiih 
ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate several functions at once and likely impact the structural integrity of the large mRNP assemblies, these...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD035153 | Pride
Rna-binding sites Npl3 Sr-like protein Mrnp assembly Structure Xl-ms Rna-binding proteins Nmr Mrna Gene expression 
ProteomicsIdentification of a novel class of cytotoxic granules as origin of supramolecular attack particles in T lymphocytes
Cytotoxic T lymphocytes (CTL) kill malignant and infected cells via cytotoxic proteins such as granzyme B (GzmB) that are released into the immunological synapse in the form of ~110 nm supramolecular attack particles (SMAPs). However, it is not known where SMAPs are stored in the cell and how they a...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-04 | PXD025055 | Pride
Exocytosis Proteomics Snare proteins Immunological synapse Cytotoxic granules Single core granules Supramolecular attack particles Total internal reflection fluorescence microscopy Correlative light and electron microscopy Multi core granules 
ProteomicsHypoxia-induced changes in SUMO conjugation affect transcriptional regulation under low oxygen
Recent data support that sumoylation, the covalent attachment of the Small Ubiquitin-related MOdifier (SUMO) to proteins, is involved in the activation of the hypoxic response and the ensuing signalling cascade. To gain insights into differences of the SUMO1 and SUMO2/3 proteome of HeLa cells under ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-03 | PXD010946 | Pride
Mass spectrometry Silac Hypoxia Sumo 
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