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ProteomicsHydrogen Deuterium Exchange of PDK1
This project consisted of three HDX-MS experiments. First, we compared the dimeric PDK1(SKD-PIF) to monomeric PDK1(SKD) and mapped the differences in deuterium incorporation onto the dimer model. We then compared the deuterium incorporation kinetics for the kinase (PDK1(SKD)) and PH (PDK1(PH) domain...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD027401 | Pride
Hdx-ms pdk1 hdx 
ProteomicsHDX-MS analysis of NKp46 with next gen antibodies
Eptiopes of 7 unique antibodies produced by two complementary in vivo platforms utilizing a common light chain, in a chicken (OmniClic) or rat (OmniFlic) host species on NKp46 were analysed using HDX-MS. NKp46 was used as a model target antigen due to its use in emerging Natural Killer (NK) immune e...
ORGANISM(S): Homo sapiens (Human) 
2025-11-27 | PXD069455 | Pride
Epitope Antibody Nkp46 Hdx 
ProteomicsDynamics of allosteric regulation of the phospholipase C-gamma isoform
we used hydrogen deuterium exchange masss spec (HDX-MS) to define the exchange kinetics of PLC-y1 as it interacts with the kinase domain of FGFR1, and liposomes containing PIP2, in order to understand how PLC-y isozymes operate at membranes. We show that the binding of FGFR1k to PLC-y1 increases deu...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-06-14 | PXD030492 | Pride
Hdxms Plc 
ProteomicsHDX-MS analysis of a TTC7B specific nanobody to inhibit EFR3 mediated PI4KA recruitment
Phosphatidylinositol 4 kinase III (PI4KIII/PI4KA) is an essential lipid kinase that is critical for regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane through targeted recruitment by the proteins EFR3A and EFR3B, with these binding to the PI4KA accessory protein...
ORGANISM(S): Homo sapiens (Human) 
2025-11-20 | PXD066213 | Pride
Pi4ka Hdx-ms Nanaobody Ttc7b 
ProteomicsAn HDX-MS optimised approach to characterise nanobodies as tools for biochemical and structural studies
Given the far-reaching applications of nanobodies, a stream-lined approach to characterizing nanobodies tailored to its intended application is warranted. Camelids can produce a large number of antibodies that can bind to a host of epitopes spanning the entire protein of interest, making identificat...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025207 | Pride
Hdx-ms Pi3k Nanobody 
ProteomicsHDX-MS analysis of PLIN3 membrane binding
Hydrogen deuterium exchange mass spectrometry of PLIN3 in the presence of three different membrane vesicles to analyze structural changes induced by membrane binding.
ORGANISM(S): Homo sapiens (Human) 
2023-04-14 | PXD025717 | Pride
Hdx Hdx-ms Plin3 
ProteomicsHDX-MS of TRAPPII reveals a conserved Rab binding interface
We used hydrogen deuterium exchange mass spectrometry (HDX-MS) to define the interface of TRAPPII with three Rab GTPases (Rab1, Rab11, Rab43). These experiments were performed in the presence of EDTA to generate a nucleotide free stabilised Rab-GEF complex. Significant decreases in deuterium incorpo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-11 | PXD020890 | Pride
Hdx-ms Trappii Gef Rab 
ProteomicsHydrogen Deuterium Exchange of PIKfyve construct with PI3P vesicles and inhibitors
We used HDXMS to study the confomational changes induced in a PIKfyve construct upon binding PI3P membrane and a variety of inhibitors. Here we showed a significant protection in deuterium exchange upon binding all inhibitors, and regions of protection and exposure upon binding PI3P membrane.
ORGANISM(S): Homo sapiens (Human) 
2025-10-15 | PXD044706 | Pride
Hdxms Apy0201 Ym-201636 Momipp Pikfyve Pi3p Apilimod 
ProteomicsHDX-MS analysis of p110a in complex with regulatory subunits
HDX-MS was used to map the changes in conformational dynamics in the catalytic subunit of PI3Ka, p110a, in complex with the following five reguatory subunits: p85a, p85b, p55g, p55a, p50a. Apo and pY activated states were also compared between the three splice variants encoded by PIK3R1. No signific...
ORGANISM(S): Homo sapiens (Human) 
2025-11-20 | PXD069057 | Pride
Hdx-ms Pi3ka P85 
ProteomicsIn vitro reconstitution of Sgk3 activation by phosphatidylinositol-3-phosphate
Serum- and glucocorticoid-regulated kinase 3 (Sgk3) is activated by the phospholipid 19 phosphatidylinositol-3-phosphate (PI3P) downstream of growth factor signaling and 20 by Vps34-mediated PI3P production during autophagy. Upregulation of Sgk3 activity 21 has been linked to a number of human cance...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025327 | Pride
Kinase Sgk3 Hdx-ms Pi3p 
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