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ProteomicsEffect of the combined loss of tomosyn (STXBP5) and tomosyn-2 (STXBP5L) on the proteome of primary mouse hippocampal neurons
Tomosyn (STXBP5) is a non-canonical SNARE protein enriched in many secretory cells and implicated in regulation of exocytosis. In neurons, loss of tomosyn affects fusion of synaptic vesicles. Here, we examined the impact of the loss of tomosyn (STXBP5) and its close paralog, tomosyn-2 (STXBP5L), on ...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-19 | PXD038442 | Pride
Tomosyn Stxbp5 Swath Neuron Synapse Mouse Primaryculture 
ProteomicsEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest popu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-11 | PXD055978 | Pride
Human Tmt Endosomes Ineurons Lysosomes 
ProteomicsReduced mGluR5 activity modulates mitochondrial function in the synapse
The metabotropic glutamate receptor 5 (mGluR5) is a G-protein coupled receptor with an important role in synaptic function at multiple levels. In physiological conditions, mGluR5 is an essential modulator of synaptic plasticity, learning and memory; whereas in pathological conditions, it is an ackno...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-27 | PXD023809 | Pride
Mitochondria Dia Swath Brain Metabotropic glutamate receptor 5 Synapse Mass spectrometry Mice Mglur5 Ctep 
ProteomicsUnbalanced protein expression contributes to pathology in Fragile X Syndrome
Dysregulated protein synthesis is a core pathogenic mechanism in Fragile X Syndrome (FX). The mGluR Theory of FX predicts that pathological synaptic changes arise from the excessive translation of mRNAs downstream of mGlu1/5 activation. Here, we use a combination of CA1 pyramidal neuron-specific Tra...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-16 | PXD031932 | Pride
Synapse Brain Hippocampus Swath Fmr1 Mglur5 Ribosome Mouse Proteasome Fragile x 
ProteomicsSHANK2A and SHANK2A(R841X) mouse brain SWATH quantitative proteomics
The high molecular complexity of the SHANK scaffold and its fundamental role in neural circuitries governing social behavior is not well understood. Here we analyzed two newly generated transgenic mouse lines with the SHANK2A wild-type isoform and the other with SHANK2A carrying a SHANK2A(R841X) poi...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-23 | PXD016665 | Pride
Lc-msms Shank2a Brain Swath Hippocampus Mouse 
ProteomicsBlue Native PAGE co-fractionation of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054684 | Pride
Co-fractionation Human Endosomes Bn-page Endomap 
ProteomicsCross-linking mass spectrometry of purified human endosomes (DHSO, DMTMM)
EndoMAP aims to systematically dissect the human endosomal protein complexes. DHSO/DMTMM cross-linking experiment was performed on freshly prepared endosomal pellets from HEK293 cells to capture protein interactions and structures in purified organelles.
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD059547 | Pride
Dhso Cross-linking Human Dmtmm Endosomes Endomap 
ProteomicsCharacterization of endosomes and interactions from TMEM230 and TMEM9/9B mutants in human neurons
EndoMAP aims to systematically dissect the human endosomal protein complexes. To examine TMEM9 and TMEM230 interactions, we generated TMEM230 and TMEM9 knockouts (KOs) using CRISPR/Cas9 in human embryonic stem (ES) cells and converted the cells to induced neurons (iNeurons). TMEM230 and TMEM9 were i...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054765 | Pride
Tmt Human Tmem9 Endosomes Ineurons Tmem230 Endomap 
ProteomicsCross-linking mass spectrometry of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. Cross-linking experiments were performed on two biologically independent replicates of freshly prepared endosomal pellets from HEK293 cells to capture protein interactions and structures in purified organelles.
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054728 | Pride
Cross-linking Human Endosomes Dsso Endomap 
UnknownEndoMAP.v1 charts the structural landscape of human early endosome complexes.
Not available
S-EPMC12222028 | biostudies-literature
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