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ProteomicsULK1-dependent phosphorylation of RIPK1 within the intermediate domain
Mass-spectrometric analysis of an in vitro kinase assay using recombinant active ULK1 (Sigma-Aldrich, SRP5096) as kinase and GST-RIPK1(250-475) as substrate
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD018078 | Pride
Ulk1 Ripk1 
ProteomicsO-GlcNAcylation stabilizes ULK1 by inhibiting chaperone-mediated autophagy upon HPV infection
Human papillomaviruses (HPVs) are conducive to a fraction of head and neck squamous cell carcinoma (HNSCC). Previously we demonstrated that HPV16 oncogene E6 or E6/E7 transduction increases the abundance of O-linked GlcNAcylation (O-GlcNAc) transferase (OGT). Herein we focus on the effects of O-GlcN...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD031095 | Pride
O-glcnac  ulk1  hpv  hnscc  macroautophagy  chaperone-mediated autophagy 
ProteomicsULK1 as a novel therapeutic target to enhance immune checkpoint therapies against melanoma
Immune checkpoint inhibitors (ICIs) have transformed the treatment of melanoma. However, the majority of patients have primary or acquired resistance to ICIs, limiting durable responses and patient survival. Interferon-gamma (IFNg) signaling and the expression of IFNg-stimulated genes correlate with...
ORGANISM(S): Homo sapiens (Human) 
2023-02-02 | PXD035347 | Pride
Irf1 Interferon-gamma Ulk1 Melanoma Pd-l1 Signaling Immune checkpoint inhibitors 
ProteomicsULK1-Driven Autophagy Modulation Alters Tumor-Promoting Pathways in Triple-Negative Breast Cancer
This dataset supports a study investigating the proteomic consequences of pharmacological ULK1 modulation in triple-negative breast cancer. MDA-MB-231 cells were treated with the ULK1 activator LYN-1604 or the ULK1 inhibitor MRT68921 at EC50 concentrations (29.8 µM and 2.6 nM, respectively) for 24 h...
ORGANISM(S): Homo sapiens (Human) 
2026-03-09 | PXD072955 | Pride
Ulk1-driven autophagy modulation alters tumor-promoting pathways in triple-negative breast cancer 
ProteomicsSalvia chinensia Benth induces autophagy in esophageal cancer cells via AMPK/ULK1 signaling pathway
Salvia chinensia Benth. (Shijianchuan in Chinese, SJC) has been used as a traditional anti-cancer herb. SJC showed good anti-esophageal cancer efficacy based on our clinical application. However, the current research on SJC is minimal, and its anti-cancer effect lacks scientific certification. This ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD035823 | Pride
Salvia chinensia benth.  herbal medicine  esophageal cancer  autophagy  proteomics  ampk/ulk1 pathway  sensgfp-stubrfp-lc3 
ProteomicsProximity labelling identification of ULK1 wild-type (WT) and ULK1-K46I (kinase dead) interactors upon mitochondria tethering in U2OS WT cells
This project involved generating three stable cell lines using a Flp-In system, each containing specific integrated constructs: "FKBPx2-myc-APEX2-ULK1", "FKBPx2-myc-APEX2-ULK1-K46I" (kinase-dead mutant), and "FKBPx2-myc-APEX2" (control). All cell lines additionally express an outer mitochondrial mem...
ORGANISM(S): Homo sapiens (Human) 
2026-04-24 | PXD068381 | Pride
Mitophagy Autophagy Ulk1-k46i Ulk1 Proximity labeling Apex2 
ProteomicsAPEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
Fip200 Mitophagy Autophagy Frb-rapalog-fkbp interaction Ulk1 Apex2 Er 
ProteomicsA Unique Signaling Feedback Circuit Defines Interferon Responses in Myeloproliferative Neoplasms
Type I interferons (IFNs) are key initiators and effectors of the immune response against malignant cells and can also directly inhibit tumor growth. IFN is highly effective in the treatment of myeloproliferative neoplasms (MPNs), but the mechanisms of action are unclear and it remains unknown why ...
ORGANISM(S): Homo sapiens (Human) 
2022-01-10 | PXD021748 | Pride
Ulk1 Interferon Neoplasm Rock2 Rock1 
ProteomicsPhosphosite mapping on PI3Kc1 by ULK1 and TBK1 kinases.
Autophagy is a highly conserved catabolic process which degrades and recycles intracellular components. To accomplish this task the surplus material (cargo) is sequestered in a double membranous vesicle termed autophagosome. To initiate the formation of autophagosome autophagy machinery is recruited...
ORGANISM(S): Homo sapiens (Human) 
2023-05-08 | PXD039663 | Pride
Pi3kc3-c1 Phosphorylation Ulk1 Tbk1 
ProteomicsULK1/2 DKO MEF phosphoproteomics
Autophagy is a mechanism by which intracellular cargo are catabolised in the lysosome. It involves the formation of double membraned organelles termed autophagosomes, a complex process orchestrated by the serine/threonine kinase ULK complex and class III PI3 kinase VPS34. Unbiased screens for ULK su...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-15 | PXD022228 | Pride
Phosphoproteomics Vps15 Vps34 P150 Pi3 kinase Autophagy Lc-ms/ms Pik3r4 Autophagosome Prkag2 Ulk2 Tmt Ulk1 Silac Amino acid starvation Mouse embryonic fibroblasts Orbitrap ulk1 
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