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ProteomicsInteraction of Hsp70 Ssb1 and atypical Hsp70 Ssz1
Our goal was to better understand the interaction between the Hsp70 Ssb1 and the atypical Hsp70 Ssz1, which we had uncovered by in vivo site-specific Bpa crosslinking. Ssz1 having Bpa incorporated was purified and incubated with purified Ssb1 and the J-domain protein Zuo1 during activation of photo ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-08-26 | PXD024065 | Pride
Yeast Ssb1 Mass spectrometry Photo crosslinking Ssz1 
ProteomicsCross-linking mass spectrometry analysis of trimeric complex of CRM1, SNP1 and RanGTP cross-linked with BS3
An X-ray crystallography structure of the trimeric nuclear export complex of CRM1, SNP1 and RanGTP is available on the PDB database with the ID 3GJX. To get an XL-MS dataset of a clean sample of a stable protein complex for benchmarking purposes, another sample from the protein expression, purificat...
ORGANISM(S): Escherichia coli 
2020-10-20 | PXD014359 | Pride
Technical dataset 
ProteomicsIdentification of HJURP binding determinants on the Mis18 complex using photo-cross-linking and mass spectrometry
In the early G1 phase of the cell cycle, human HJURP is recruited to centromeres by the Mis18 complex, which consists of Mis18α, Mis18β and M18BP1. This interaction of HJURP with the Mis18 complex is essential for CENP-A deposition and the preservation of centromere identity. To identify the binding...
ORGANISM(S): Homo sapiens (Human) 
2019-08-01 | PXD013339 | Pride
Photo-cross-linking Q-exactive Abk Human Kinetochore Centromere Protein complex Bpa Stavrox 
ProteomicsDirect evidence of the presence of cross-linked Aβ dimers in the brains of Alzheimer’s disease patients
Brain-derived amyloid-β (Aβ) dimers are associated with Alzheimer´s disease (AD). However, their covalent nature remains controversial. This feature is relevant, as a covalent cross-link would make brain-derived dimers (native dimers) more synaptotoxic than Aβ monomers and would make them suitable c...
ORGANISM(S): Homo sapiens (Human) 
2021-05-13 | PXD005657 | Pride
Alzheimer’s disease Amyloid-β Oxidative stress Cross-linked dimers 
ProteomicsPTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport
The kinesin-3 KIF1C is a fast organelle transporter implicated in the transport of dense core vesicles in neurons and the delivery of integrins to cell adhesions. Here we report the mechanisms of autoinhibition and release that control the activity of KIF1C. We show that the microtubule binding surf...
ORGANISM(S): Homo sapiens (Human) 
2019-06-24 | PXD013939 | Pride
Autoinhibition Ptpn21 Hook3 Kif1c 
ProteomicsHuman mitochondrial cysteine desulfurase complex
Cysteine desulfurase plays central role in mitochondrial iron-sulfur cluster biogenesis not only by providing sulfur through the catalysis of L-cysteine to L-alanine but also by serving as the platform for the assembly of other components of the biosynthetic machinery, including ISCU, frataxin, and ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-11 | PXD009079 | Pride
Human Cysteine desulfurase Cx-ms 
ProteomicsHuman mitochondrial cysteine desulfurase complex data set 2
Cysteine desulfurase plays central role in mitochondrial iron-sulfur cluster biogenesis not only by providing sulfur through the catalysis of L-cysteine to L-alanine but also by serving as the platform for the assembly of other components of the biosynthetic machinery, including ISCU, frataxin, and ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-25 | PXD009128 | Pride
Mitochondrial cysteine desulfurase Xc-ms 
ProteomicsPeriostin is a disulfide bonded homodimer and forms a complex with fibronectin in human skin
The protein periostin is a matricellular protein that is expressed in connective tissue. It is composed of five globular domains arranged in an elongated structure with an extensive disordered C-terminal tail. Periostin contains eleven cysteine residues, of which one is unpaired, and the rest forms ...
ORGANISM(S): Homo sapiens (Human) 
2025-01-22 | PXD045285 | Pride
Wound healing Periostin Fibronectin Extracellular matrix 
ProteomicsIdentification of Cross-linking partners of BpA modified p97 by photo cross-linking MS
We wanted to see where and how the protein p97 interacts with its partner proteins p37, PPI,Sds22 and I3. To this end a p97 derivative with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) was generated. After incubation with the partner proteins, photocross-linking and trypti...
ORGANISM(S): Homo sapiens (Human) 
2023-07-10 | PXD039939 | Pride
Aaa protease Bpa crosslinking P97 
Proteomicsdentification of Cross-linking partners of BpA modified p37 and p97 by photo cross-linking MS
we wanted to see where and how the protein p37 interacts with its partner proteins p97, PPI,Sds22 and I3. To this end p37 derivatives with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) were generated. After incubation with the partner proteins, photocross-linking and trypt...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021182 | Pride
P37 Ppi Sds22 Photo cross-linking P97 
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