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UnknownFunctional characterization of the lysosomal membrane protein TMEM192 in mice.
Not available
S-EPMC5546430 | biostudies-literature
UnknownThe TMEM192-mKeima probe specifically assays lysophagy and reveals its initial steps.
Not available
S-EPMC10558291 | biostudies-literature
UnknownActivation of lysophagy by a TBK1-SCF<sup>FBXO3</sup>-TMEM192-TAX1BP1 axis in response to lysosomal damage.
Not available
S-EPMC11775327 | biostudies-literature
ProteomicsLyso-IP from Wdr7 iKO MEFs
Lysosomes were enriched from Wdr7 iKO MEFs stably expressing TMEM192-3xHA (Bait) or TMEM192-2xFlag (Ctrl) via lysosomal immunoprecipitation (Lyso-IP) using PierceTM anti-HA magnetic beads to analyze changes in the lysosomal protein content upon induced depletion of Wdr7.
ORGANISM(S): Mus musculus (Mouse) 
2026-06-25 | PXD070950 | Pride
Lyso-ip Mefs V-atpase Tmem192 Lysosomes Iko Wdr7 
ProteomicsMass Spectrometry Profiling of Lysosomal IP under Normoxia and Hypoxia-Reoxygenation
This project employed TMEM192-3×Flag-mediated lysosome immunoprecipitation (Lyso-IP) followed by mass spectrometry to investigate lysosomal proteomes under normoxia and hypoxia/reoxygenation (H/R) stress. Our study focuses on the mechanisms of lysosomal renewal, revealing that damaged lysosomes unde...
ORGANISM(S): Homo sapiens (Human) 
2026-06-03 | PXD078485 | Pride
Human B-fission 
ProteomicsTwo step enrichment facilitates background reduction for proteomic analysis of lysosomes
Lysosomes comprise the main degradative compartment of almost all mammalian cells and are involved in various cellular functions, most of which are catalyzed by the lysosomal proteome. Lysosomal proteins are low abundant, complicating their analysis by mass spectrometry-based proteomics. To increase...
ORGANISM(S): Homo sapiens (Human) 
2024-08-01 | PXD048696 | Pride
Lysosomes  lysosome enrichment  superparamagnetic iron oxide particles (spions)  tmem192 Mass spectrometry  proteomics  organelles Hek 293 
UnknownTazarotene-Induced Gene 1 Enhanced Cervical Cell Autophagy through Transmembrane Protein 192.
Not available
S-EPMC5223105 | biostudies-literature
ProteomicsSystematic comparison of strategies for the enrichment of lysosomes by data independent acquisition
We describe a comparison of individual approaches for the enrichment of lysosomes comparing four different strategies using data independent acquisition (DIA). As a benchmark, we chose a crude enrichment strategy, which is the centrifugation of the PNS at 20,000 x g termed organelle enriched pellet ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-21 | PXD015098 | Pride
Enrichment methods Lysosomes Data independent acquisition (dia) 
ProteomicsLysosomal proteome characterization during TRPML1 activation by MLSA5 in human cancer cells
To capture the changes in the lysosomal proteome upon TRPML1 activation, achieved using a small molecule agonist MLSA5, human cancer cells, with or without gene-editing, stably expressing TMEM192-mRFP-3xHA (Lyso-IP), were treated with MLSA5 and Lyso-IP samples were prepared and processed for TMT-MS ...
ORGANISM(S): Homo sapiens (Human) 
2025-04-22 | PXD054968 | Pride
Mlsa5 Tmt A101d V-atpase Lysosome Trpml1 Dmxl2 Dmxl1 Lyso-ip U2os Atg16l1 
ProteomicsLysosomal protein atlas of brain cell types identifies SLC45A1-associated disease as a lysosomal disorder
We identify dozens of novel lysosomal proteins across brain cells, including those linked to disease. Notably, we found that SLC45A1, mutations in which cause a monogenic neurological disease, is a neuron-specific lysosomal protein. Here, we identify potential interaction partners of SLC45A1, but al...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-10-09 | MSV000096053 | MassIVE
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