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ProteomicsEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest popu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-11 | PXD055978 | Pride
Human Tmt Endosomes Ineurons Lysosomes 
ProteomicsLyso-IP from Wdr7 iKO MEFs
Lysosomes were enriched from Wdr7 iKO MEFs stably expressing TMEM192-3xHA (Bait) or TMEM192-2xFlag (Ctrl) via lysosomal immunoprecipitation (Lyso-IP) using PierceTM anti-HA magnetic beads to analyze changes in the lysosomal protein content upon induced depletion of Wdr7.
ORGANISM(S): Mus musculus (Mouse) 
2026-06-25 | PXD070950 | Pride
Lyso-ip Mefs V-atpase Tmem192 Lysosomes Iko Wdr7 
UnknownEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons.
Not available
S-EPMC11463543 | biostudies-literature
ProteomicsLysosomal proteome characterization during TRPML1 activation by MLSA5 in human cancer cells
To capture the changes in the lysosomal proteome upon TRPML1 activation, achieved using a small molecule agonist MLSA5, human cancer cells, with or without gene-editing, stably expressing TMEM192-mRFP-3xHA (Lyso-IP), were treated with MLSA5 and Lyso-IP samples were prepared and processed for TMT-MS ...
ORGANISM(S): Homo sapiens (Human) 
2025-04-22 | PXD054968 | Pride
Mlsa5 Tmt A101d V-atpase Lysosome Trpml1 Dmxl2 Dmxl1 Lyso-ip U2os Atg16l1 
UnknownEndo-IP and lyso-IP toolkit for endolysosomal profiling of human-induced neurons.
Not available
S-EPMC11670117 | biostudies-literature
ProteomicsHRMS1 DIA and DDA of Lyso-IP inputs from mouse gut epithelium and stem cells
These mouse gut lysates contained three sets, with Set A including 21 DDA experiments and 21 DIA experiments, Set C including 24 DDA and 24 DIA experiments, and Set G including 27 DDA RAWs and 26 DIA RAWs (one of the Set G DDA experiments was run twice). Experiment Set A was intended to compare lys...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-07-26 | MSV000095444 | MassIVE
ProteomicsTagless LysoIP method for molecular profiling of lysosomal content in clinical samples
Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling lysosomal content using tag-based lysosomal immunopurification (LysoIP) in cell and animal models allowed major discoveries in ...
ORGANISM(S): Homo sapiens (Human) 
2025-01-31 | PXD052082 | Pride
Metabolomics Proteomics Dia Lysosomal storage disorder Lipidomics Lysosome Mass spectrometry 
ProteomicsLOSS OF THE BATTEN DISEASE PROTEIN CLN3 LEADS TO MIS-TRAFFICKING OF M6PR AND DEFECTIVE AUTOPHAGIC-LYSOSOMAL REFORMATION
Batten disease, one of the most devastating types of neurodegenerative lysosomal storage disorders, is caused by mutations in CLN3. Here, we show that CLN3 is a vesicular trafficking hub connecting the Golgi and lysosome compartments. Proteomic analysis reveals that CLN3 interacts with several endo-...
ORGANISM(S): Homo sapiens (Human) 
2023-06-20 | PXD031582 | Pride
Human Cln3 Batten Lysosome 
UnknownSpatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics.
Not available
S-EPMC9573879 | biostudies-literature
ProteomicsMass Spectrometry Profiling of Lysosomal IP under Normoxia and Hypoxia-Reoxygenation
This project employed TMEM192-3×Flag-mediated lysosome immunoprecipitation (Lyso-IP) followed by mass spectrometry to investigate lysosomal proteomes under normoxia and hypoxia/reoxygenation (H/R) stress. Our study focuses on the mechanisms of lysosomal renewal, revealing that damaged lysosomes unde...
ORGANISM(S): Homo sapiens (Human) 
2026-06-03 | PXD078485 | Pride
Human B-fission 
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