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ProteomicsHiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line
Here we present high resolution data from two cancer cell lines. A human cell line, A431 was treated with a drug at different time points and then fractionated into its different subcellular compartments (nuclear, organellar, soluble,whole cell). Protein extracts were trypsinized, peptides separated...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2013-09-27 | PXD000065 | Pride
Mouse Lc-ms Hirief Isoelectric focusing Cancer N2a Human A431 
ProteomicsProteomic screen reveals Fbw7 as a modulator of the NF-kB pathway
Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample...
ORGANISM(S): Homo sapiens (Human) 
2012-06-29 | PXD000005 | Pride
Lc-msms orbitrap human colon epithelium fbw7 hirief 
TranscriptomicsRNA-seq of A431 cell line after gefitinib treatment
RNA sequencing of A431 cell line samples before and after gefitinib treatment, at 0, 2, 6 and 24 hours, was performed in order to characterize the cell line's early and late response to this drug, and to compare against proteomics (mass spectrometry) characterization of the cell line using the same ...
ORGANISM(S): Homo sapiens 
2016-12-01 | E-MTAB-5285 | biostudies-arrayexpress
ProteomicsProteomics on post-mortem human brain tissue of patients with Alzheimer`s, Parkinson`s and Lewy body dementias, Brodmann areas (BA) 9, 24 and 40.
We have used HiRIEF (High Resolution Isoelectric Focusing) LC-MS proteomics (Branca et al., 2014, PMID: 24240322) with isobaric tags (TMT10plex) to compare 32 post-mortem human brains in the prefrontal cortex (Brodmann areas 24 and 40) of prospectively followed patients with Alzheimer`s disease (AD)...
ORGANISM(S): Homo sapiens (Human) 
2024-06-10 | PXD047324 | Pride
Human Post-mortem Alzheimer Brain Lewy body dementia Tmt Autopsy Parkinson Brodmann area Hirief Lcms proteomics 
ProteomicsDOPA formation on tyrosine Y150 of Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis.
With a crystal structure and mass spectrometry on recombinantly expressed and purified Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis we show that this protein has a modified DOPA (3,4-Dihydroxy-L-phenylalanine) in the active site analogous to what was observed for the VPK varia...
ORGANISM(S): Gardnerella vaginalis 
2023-09-08 | PXD045227 | Pride
Qexactive hf 3 Ptm Gardnerella vaginalis 4-dihydroxyphenylalanine Tyrosine Ribonuclotide reductase Dopa 
ProteomicsImmediate adaption – Quantitative Proteomics of BCL6 silencing and EGFR TK inhibition
We aimed to identify the response mechanism to EGFR tyrosine kinase inhibition. A431 cells were transfected with mock siRNA or BCL6 siRNA. Cells were then treated with gefitinib. Harvesting was done at 0h, at 24h and at 48h. Two TMT10plex sets were organized as follows: TMT set1: mock 0h (3x, channe...
ORGANISM(S): Homo sapiens (Human) 
2020-12-09 | PXD016605 | Pride
Human Bcl6 sirna Tmt proteomics Lc-ms Time course Egfr inhibition Gefitinib A431 cells 
ProteomicsProteomics of U1810 cells upon treatment with microRNAs with an AAGUGC seed motif.
microRNA dysregulation is a common feature of cancer cells, but the complex roles of microRNAs in cancer are not fully elucidated. Here we used functional genomics to identify oncogenic microRNAs in non-small cell lung cancer and to evaluate their impact on response to EGFR targeting therapy. Our da...
ORGANISM(S): Homo sapiens (Human) 
2016-06-28 | PXD004163 | Pride
Human Mirna Lc-ms Microrna U1810 Hirief 
ProteomicsLabel free HiRIEF deep proteomics of A431 human vulvar cancer cell line.
Human A431 cells were lysed. Protein extracts were trypsinized, peptides separated by HiRIEF (high resolution isoelectric focusing) and analysed by LC-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD000542 | Pride
Lc-ms Hirief Isoelectric focusing Cancer Human A431 
ProteomicsProteomics on post-mortem human brain tissue of patients with Alzheimer`s, Parkinson`s and Lewy body dementias.
We have used HiRIEF (High Resolution Isoelectric Focusing) LC-MS proteomics with isobaric tags (TMT10plex) to compare 32 post-mortem human brains in the prefrontal cortex (Brodmann area 9) of prospectively followed patients with Alzheimer`s disease (AD), Parkinson`s disease with dementia (PDD), deme...
ORGANISM(S): Homo sapiens (Human) 
2017-12-08 | PXD006122 | Pride
Synaptic Lewy body Proteomics Brain Prefrontal cortex Alzheimer Brodmann area 9 Parkinson Hirief Lcms Human 
ProteomicsImmunopeptidome of Human Renal Cell Carcinoma (RCC) derived cells infected with oncolytic viruses.
In this study we demonstrate the effect of oncolytic adenoviruses armed with CXCL9, CXCL10 and IL-15 to infect cancer cells, secrete the encoded protein and drive gradient-dependent T-cell attraction. Our in vivo validation demonstrated an increased intratumoral CD4+ and CD8+ T-cell infiltration fol...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD051880 | Pride
Oncolytic adenovirus Human Pepticrad Ligandome Immunopeptidome Hla-i Mhc-i Rcc Il-15 Cxcl10 Cxcl9 
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